Biological method for preparing heme iron not derived from porcine blood

A heme iron, biological technology, applied in iron group organic compounds without C-metal bonds, iron organic compounds, organic chemistry, etc., can solve problems such as hindering the use of iron supplements, residues, infections, etc.

Active Publication Date: 2019-09-06
INTRON BIOTECHNOLOGY INC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The heme iron thus prepared has many problems that are not present in non-heme iron, such as the risk of infection from zoonotic sources, livestock growth hormone contamination, and antibiotic residues
Moreover, the preparation of heme iron as described above involves the production of heme iron from blood obtained from slaughtered animals such as pigs, which is prohibited in Islam, and thus the heme iron prepared as above is not halal, undesirably hindering its Uses as Iron Supplements for Muslims

Method used

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  • Biological method for preparing heme iron not derived from porcine blood
  • Biological method for preparing heme iron not derived from porcine blood
  • Biological method for preparing heme iron not derived from porcine blood

Examples

Experimental program
Comparison scheme
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Embodiment 1

[0032] Embodiment 1: Preparation of heme iron producing strain

[0033] Regarding the heme biosynthetic pathway in the microorganisms of the present invention, heme iron producing strains were prepared through several trial and error steps. The heme iron-producing strain thus prepared is E. coli DH5α pLEX_HMD, which is a DH5α-based E. coli through a plasmid designed for heme biosynthesis specifically containing the self-expressing promoter P L , ALA synthase gene (HemA), NADP-dependent malic acid enzyme gene (MaeB) and dicarboxylate transporter gene (DctA), the strain thus prepared was deposited in Korea Bioscience and Korean Type Culture Collection of the Institute of Biotechnology (accession number: KCTC 13173BP).

[0034] Based on the comparison of its heme iron production ability with strains prepared using MG1655, Top10, BL21, and Rosetta gami, the production strain E. coli DH5αpLEX_HMD showed excellent heme iron production ability compared with other E. coli strains c...

Embodiment 2

[0040] Embodiment 2: Optimization of heme iron production conditions

[0041] Using the production strain Escherichia coli DH5αpLEX_HMD prepared in Example 1, the production process was optimized, including the optimization of the medium composition. figure 1 The results of optimizing the culture temperature as one of the conditions used to optimize the production process are shown. The production conditions of heme iron finally established through several methods to obtain the optimal production conditions are summarized below.

[0042] [Table 2]

[0043] Optimal conditions for heme iron production

[0044]

[0045]

Embodiment 3

[0046] Embodiment 3: Optimization of heme iron purification conditions

[0047] In this example, a heme iron purification method was developed and its specific conditions were optimized. The finally established purification conditions for heme iron are as follows.

[0048] The culture solution of the heme iron-producing strain Escherichia coli DH5αpLEX_HMD was centrifuged at 3,000 g for 15 minutes at 4° C. to recover cells thereof. The cells thus recovered were washed twice by suspending in PBS (phosphate-buffered saline) followed by centrifugation. The finally recovered cells were naturally dried for about 30 minutes and then weighed. Typically, 40 to 50 g of cells can be recovered from 5 L of culture medium. Heme iron was extracted by adding cold acid-acetone to the recovered cells. Here, the cold acid-acetone used was prepared by mixing 998 ml of acetone with 2 ml of hydrochloric acid at -20°C. Addition of cold acid-acetone was performed by adding 1 L of cold acid-ac...

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Abstract

The present invention relates to heme iron not derived from porcine blood and a method for preparing same, and more specifically, to a method for preparing heme iron not derived from porcine blood through biological preparation steps, a method for preparing salts thereof, and an iron supplement containing the salt prepared thereby as an active ingredient.

Description

technical field [0001] The present invention relates to animal-derived component-free heme iron and a method for its preparation, and more particularly to a method for the biological preparation of heme iron not derived from pig blood, characterized by the absence of animal-derived components, the preparation of A method of said salt of heme iron, and an iron supplement containing the salt thus prepared as an active ingredient. Background technique [0002] Iron (Fe) is a trace element that plays a crucial role in oxygen transport in the body and is an essential component of hemoglobin, myoglobin, cytochromes, iron / sulfur proteins and biomolecular structures. The total amount of iron in the body is about 3 to 4 g, of which 60% to 65% is bound to hemoglobin in circulating red blood cells and the remaining 30% to 35% exists as iron stores (ferritin). Iron is also present in the form of tissue iron and serum iron (transferrin) and, in addition, in small amounts in muscle myogl...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P9/00C12N1/20C07F15/02A61K33/26
CPCA61K33/26C07F15/02C12N1/20C12P9/00C12P17/182C07F15/025C07D487/22
Inventor 尹圣君朴俊因黄孙玄俊苏云康桑贤
Owner INTRON BIOTECHNOLOGY INC
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