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Method for simultaneously detecting multiple lipid-soluble vitamin contents in blood

A technology for fat-soluble vitamins and vitamins, which is applied in the field of chemistry and can solve problems such as methods for the simultaneous determination of multiple fat-soluble vitamins.

Active Publication Date: 2019-09-06
BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0009] At present, HPLC (High Performance Liquid Chromatography, high performance liquid chromatography) can be used to detect a single fat-soluble vitamin, but there is no method for the simultaneous determination of multiple fat-soluble vitamins in blood

Method used

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  • Method for simultaneously detecting multiple lipid-soluble vitamin contents in blood
  • Method for simultaneously detecting multiple lipid-soluble vitamin contents in blood
  • Method for simultaneously detecting multiple lipid-soluble vitamin contents in blood

Examples

Experimental program
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Embodiment 1

[0048] Such as figure 1 As shown, the embodiment of the present invention provides a method for simultaneously detecting the content of multiple fat-soluble vitamins in blood, which may include the following steps:

[0049] Step 101: Using a high-performance liquid phase tandem mass spectrometer, under certain detection conditions, respectively detect N standard solutions to obtain the chromatograms of each standard solution, wherein any standard solution contains at least two fat-soluble substances with known concentrations. For vitamins and their internal standards, the concentration of the same fat-soluble vitamin in different standard solutions is different, and N≥3.

[0050] Step 102: In the standard solution, the ratio of the concentration of a fat-soluble vitamin to the concentration of its internal standard is a two-dimensional coordinate, and the ratio of the peak area of ​​the fat-soluble vitamin to its internal standard in the chromatogram of the standard solution ...

Embodiment 2

[0062] The embodiment of the present invention is basically the same as the embodiment 1, and the similarities will not be repeated, the difference is that: the at least two fat-soluble vitamins include: vitamin A, 25-hydroxyvitamin D2, 25-hydroxyvitamin D3, vitamin E , vitamin K1, and at least two of vitamin K2.

[0063] Please refer to Figure 2 to Figure 6 , figure 2 The chemical structural formula of vitamin A is shown, image 3 The chemical structural formula of 25-hydroxyvitamin D2 is shown, Figure 4 The chemical structural formula of 25-hydroxyvitamin D3 is shown, Figure 5 The chemical structural formula of vitamin E is shown, Figure 6 The chemical structural formula of vitamin K1 is shown.

[0064] In detail, the vitamin K2 here can be vitamin K2 (MK4), vitamin K2 (MK7), vitamin K2 (MK9). Please refer to Figure 7 , Figure 7 The chemical structural formula of vitamin K2 (MK4) is shown.

[0065] In detail, corresponding to the above step 101, before detect...

Embodiment 3

[0068] The embodiment of the present invention is basically the same as the embodiment 1, and the similarities will not be repeated. The difference is that: the number of types of the at least two fat-soluble vitamins is 6;

[0069] Before said step 101, further comprising:

[0070] Step A1: Use a pipette to pipette 90 μL of the first standard working solution, 10 μL of the second standard working solution and 10 μL of the mixed internal standard working solution into a centrifuge tube, wherein the first standard working solution contains vitamins with known concentrations A and vitamin E with known concentration, the second standard working solution contains 25-hydroxyvitamin D2 with known concentration, 25-hydroxyvitamin D3 with known concentration, vitamin K1 with known concentration and vitamin K2 with known concentration;

[0071] Step A2: After vortexing the centrifuge tube at a speed of 1500rpm-2500rpm for 1min-2min, pipette the supernatant to obtain a standard solution...

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Abstract

The invention provides a method for simultaneously detecting multiple lipid-soluble vitamin contents in blood. The method comprises the following steps: detecting at least three standard solutions byutilizing a high-performance liquid chromatograph tandem mass spectrometer so as to obtain a corresponding chromatogram, wherein each standard solution contains multiple vitamins and internal standardsubstances thereof with known concentrations; fitting to obtain standard curve equations of various vitamins according to the concentration specific value of various vitamins and the internal standard substances thereof in the standard solutions, and the peak area specific value of the vitamin and the internal standard substance in the corresponding chromatogram; treating to-be-detected blood toobtain a blood sample, and adding multiple internal standard substances with known concentrations, and performing sample pre-treatment to obtain a to-be-detected sample; detecting the to-be-detected sample under the same detection condition to obtain the chromatogram thereof; substituting the peak area specific value of each vitamin and the internal standard substance thereof in the chromatogram into the corresponding standard curve equation, and computing the content of each vitamin in the to-be-detected blood according to the concentration of the added internal standard substances thereof. Through the method provided by the invention, the contents of various lipid-soluble vitamin contents in the blood can be detected at the same time.

Description

technical field [0001] The invention relates to the technical field of chemistry, in particular to a method for simultaneously detecting the contents of various fat-soluble vitamins in blood. Background technique [0002] Fat-soluble vitamins are closely related to human health. [0003] Vitamin A is closely related to normal vision, is necessary for normal bone growth, helps cell proliferation and growth, and is very important for the normal formation, development and maintenance of epithelium. In recent years, it has been proved that vitamin A has the function of delaying or preventing pre-pathological changes, preventing chemical carcinogens, especially epithelial malignant tumors. Quantitative determination of serum vitamin A is of great significance for the diagnosis and curative effect observation of certain diseases. For example, abnormal results include: (1) Reduced: vitamin A deficiency (night blindness, dry eye, corneal softening disease), lipid malabsorption syn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/04G01N30/72G01N30/06G01N30/86G01N30/88
CPCG01N30/02G01N30/04G01N30/72G01N30/06G01N30/8634G01N30/88G01N2030/045G01N2030/062G01N2030/8822Y02A50/30
Inventor 许丽雒琴贾永娟倪君君
Owner BEIJING HARMONY HEALTH MEDICAL DIAGNOSTICS CO LTD
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