Kit for detecting and identifying Seneca valley virus and foot-and-mouth disease virus type O, A and Asial, and primers and probes thereof

Abstract

The invention discloses a kit for detecting and identifying a Seneca valley virus and foot-and-mouth disease virus type O, A and Asial, and primers and probes thereof. The kit is used for detecting and identifying a Seneca valley virus, a foot-and-mouth disease virus type O, a foot-and-mouth disease virus type A and a foot-and-mouth disease virus type Asial; and the sequences of the primers and probes are as follows: the sequences of the detection primers and probes of the Seneca valley virus are SEQ1, SEQ2 and SEQ3; the sequences of the detection primers and probes of the foot-and-mouth disease virus type O are SEQ4, SEQ5 and SEQ6; the sequences of the detection primers and probes of the foot-and-mouth disease virus type A are SEQ7, SEQ8 and SEQ9; and the sequences of the detection primers and probes of the foot-and-mouth disease virus type Asial are SEQ10, SEQ11 and SEQ12. The kit can simultaneously perform the detection and discriminating on the Seneca valley virus and the foot-and-mouth disease virus type O, A and Asial, so that operation steps can be simplified, detection time can be shortened, and detection accuracy can be enhanced.

Description

technical field [0001] The present invention relates to a kind of specific primer pair and probe that can be used for the detection of animal virus, and the quantitative detection kit that includes specific primer pair and probe. One-step TapMan real-time fluorescent quantitative PCR detection kit for Valley virus, foot-and-mouth disease virus O, A and Asia. Background technique [0002] Seneca Valley virus (SVV) is a single-stranded, non-segmented RNA virus that is the only member of the genus Seneca Valley virus in the family Picornaviridae. Since September 2015, vesicular epidemics have occurred in pigs in many parts of the American countries. Infected pigs are lame, red and hot around the crown and hoof wall, and ulcerative lesions appear. Blister-like lesions appeared, and after laboratory pathogen identification, they were ruled out as important foreign animal diseases such as foot-and-mouth disease, swine vesicular disease, and vesicular stomatitis, and were finally ...

AI Technical Summary

Problems solved by technology

In the summer of 2015, the first case of the virus was reported in Guangdong Province of my country, followed by an outbreak of the virus in Hubei Province in April 2016. The main clinical symptoms were blister-like lesions on the snout and hooves of pigs. The virus will not cause significant economic losses, but because the clinical symptoms shown after infection are indistinguishable from foot-and-mouth disease, it is urgent to establish a rapid, sensitive and specific differential diagnosis method for distinguishing Seneca Valley from foot-and-mouth disease. control of

[0003] At present, there is no report on multiple real-time fluorescent quantitative detection technology for the simultaneous identification and detection of Seneca Valley virus and Foot-and-Mouth Disease virus (Foot-and-Mouth Diseasevirus, FMDV) O, A, and Asial. The RealTime RT-PCR reaction can be performed using this kit at Continuous detection of Seneca Valley virus and foot-and-mouth disease virus O, A and Asia in the same reaction tube, simple operation, high sensitivity, and effective prevention of pollution

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