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Preparation method and application for glycosylated naringenin

A technology of naringenin and glycosylation, applied in the field of bioengineering, can solve problems such as low bioavailability of naringenin, complex extraction, separation process, research affecting pharmacological activity, etc., to achieve improved bioavailability and broad development Foreground, effect of solubility improvement

Pending Publication Date: 2019-09-06
FOSHAN GOLDEN HEALTH TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] In recent years, the research on the activity of naringenin has been very active and in-depth, but the content of naringenin in natural plants is not high, and complex extraction and separation processes are required, which directly affects the research on its pharmacological activity
At present, the methods for preparing naringenin include acid hydrolysis, aqueous phase-ethanol method, methanol method, cyclohexanol method and other methods for preparing hesperetin, but the solubility of naringenin prepared by the above method is low, making naringenin The bioavailability of vitamins is low, and the effect is not obvious

Method used

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  • Preparation method and application for glycosylated naringenin
  • Preparation method and application for glycosylated naringenin
  • Preparation method and application for glycosylated naringenin

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Experimental program
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Effect test

Embodiment 1

[0033] A kind of preparation method of glycosylated naringenin, its concrete steps comprise:

[0034] Using naringin as raw material, the reaction buffer is disodium hydrogen phosphate-sodium citrate buffer with a mass fraction of 0.9%, the pH of the reaction buffer is 5.7, the ratio of raw material to reaction buffer is 1:22, and the reaction temperature is 60 ℃, the reaction time is controlled for 15 hours, then rhamnosidase:glucosyltransferase=10:90 in the reaction system, the mass fraction of the total enzyme is 15% of the input naringin. Add 100g of 90% naringin, the glycosyl donor is glucose, and the concentration is 6g / L. In this reaction system, 155.9g (n=1-9) of polyglucose naringenin is obtained, with a yield of 96.5%. The results are shown in figure 1 , due to the difference in the number of sugar groups linked by polyglucose naringenin, a reduced peak will appear in the peak time of the HPLC graph.

Embodiment 2

[0036] A kind of preparation method of glycosylated naringenin, its concrete steps comprise:

[0037] Using naringenin as raw material, the reaction buffer is disodium hydrogen phosphate-sodium citrate buffer with a mass fraction of 0.9%, the pH of the reaction buffer is 5.7, the reaction temperature is 46°C, and the ratio of raw materials to reaction buffer is 1: 10. The reaction time is controlled for 15 hours; then in the reaction system, rhamnosidase:glucosyltransferase=90:10, the mass fraction of the total enzyme is 10% of the input naringenin; input 100g 90% naringenin, glycosyl The donor was glucose at a concentration of 1 g / L. In this reaction system, 75.1 g of monoglucoside naringenin was obtained with a yield of 93.8%.

Embodiment 3

[0039] A kind of preparation method of glycosylated naringenin, its concrete steps comprise:

[0040] Using naringin as raw material, the reaction buffer is disodium hydrogen phosphate-sodium citrate buffer with a mass fraction of 0.9%, the pH of the reaction buffer is 5.7, the ratio of raw material to reaction buffer is 1:50, and the reaction temperature is 55 ℃, the reaction time is controlled for 8 hours; then in the reaction system, rhamnosidase:glucosyltransferase=10:90, the mass fraction of the total enzyme is 25% of the input naringin; input 100g 90% naringin, glycosyl The donor was glucose at a concentration of 10 g / L. In this reaction system, 130.6 g (n=1-9) of polyglycosylated naringenin was obtained, and no naringenin was detected by HPLC.

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Abstract

The present disclosure provides a preparation method for glycosylated naringenin, wherein the preparation method is characterized by particularly comprising the steps: putting raw materials and glycosyl donors into a reaction buffer solution, mixing the feed liquid evenly, and then adding glycosidase and glycosyltransferase into the reaction system, wherein the raw materials are naringin and naringenin extracts or conversion products and derivatives thereof. The glycosylated naringenin prepared by the method can have the solubility of naringenin greatly improved by adding 1-9 glycosyl groups to a flavone matrix, not only solves the problem of poor solubility of naringenin, but also solves the problem of decomposition and absorption of naringenin in a human body, and greatly improves the bioavailability of naringenin.

Description

technical field [0001] The disclosure relates to the field of bioengineering, in particular to a preparation method and application of glycosylated naringenin. Background technique [0002] Citrus is one of the fruits with the largest planting area in my country. There are a large number of small, thin, and secondary citrus fruits every year. These fruits are generally just thrown away directly, causing a lot of waste of resources. Literature reports: the content of citrus hesperidin in the small fruit, fruit thinning and falling fruit of citrus is the highest in the whole growth cycle. Therefore, it is of high economic value to extract flavonoids from small fruit, fruit thinning and fruit drop produced during the growth of citrus. [0003] Flavonoids are treasures given to humans by nature. At present, there are about 4,000 kinds of flavonoids, which mainly exist in leaves, fruits, roots, and bark of plants. They have similar mother nucleus structures, but the structure t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/60C07H17/07A61K31/7048A61P19/10A61P39/06A61P3/10A61P11/10A61P11/14
CPCC12P19/60C07H17/07A61K31/7048A61P19/10A61P39/06A61P3/10A61P11/10A61P11/14
Inventor 周金林钟进良林丽薇黄宝华李月陈宏基
Owner FOSHAN GOLDEN HEALTH TECH CO LTD
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