Nano-preparation capable of simultaneously delivering antigen and immunoenhancer

A technology of immune enhancer and nano-preparation, applied in the field of nano-medicine, can solve the problems of poor specificity, side effects, low efficiency, etc., and achieve the effect of efficient delivery and good specificity

Inactive Publication Date: 2019-09-06
GUANGZHOU WOMEN AND CHILDRENS MEDICAL CENTER
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this method has low efficiency, poor specificity, and side effects may be accompanied by side effects
These disadvantages largely limit the application of nano-formulations

Method used

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  • Nano-preparation capable of simultaneously delivering antigen and immunoenhancer
  • Nano-preparation capable of simultaneously delivering antigen and immunoenhancer
  • Nano-preparation capable of simultaneously delivering antigen and immunoenhancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Step 1: The N-terminal of human heavy chain of human ferrtin (HFT for short) is fused with the C-terminal of the linker gbl-intein to form the recombinant protein gbl-intein c -ferrtin self-assembled into 24-mers to form nanoparticles with a diameter of 18nm.

[0037] Step 2: The C-terminus of SP70 is fused with the N-terminus of intein to form a recombinant protein, and the N-terminus of intein specifically recognizes and excises the ferritin exposed on the surface of the nanoparticle; 30 μM exogenous protein is covalent to 30 μM ferritin Cross-linking to generate nano-formulations.

Embodiment 2

[0039] Step 1: fusion of the N-terminal of the heavy chain of human ferrtin (pyrococcus furiosus heavy chain of human ferrtin, PFT) and the C-terminal of the linker gbl-intein to form the recombinant protein gbl-intein c -ferrtin self-assembled into 24-mers to form nanoparticles with a diameter of 18nm.

[0040] Step 2: The C-terminus of flagellin is fused with the N-terminus of intein to form a recombinant protein, and the N-terminus of intein specifically recognizes and excises the ferritin exposed on the surface of the nanoparticle; 60 μM exogenous protein is combined with 30 μM ferritin Valence cross-linking to generate nano-preparations.

Embodiment 3

[0042] Step 1: The N-terminal of human heavy chain ferritin is fused with the C-terminal of linker gbl-intein to form recombinant protein gbl-intein c -ferrtin self-assembled into 24-mers to form nanoparticles with a diameter of 18nm.

[0043] Step 2: The same amount of CpG and the C-terminus of the influenza virus hemagglutinin stem region are simultaneously fused to the N-terminus of the intein to form a recombinant protein. Ferritin on particle surface; 45 μM exogenous protein is covalently cross-linked with 30 μM ferritin to generate nanoformulations.

[0044] In the experiments and examples of the present invention, the genes of the following proteins and polypeptides were synthesized at Shanghai Tolo Harbor Biotechnology Co., Ltd., and the primers were synthesized at Nanjing GenScript Biotechnology Co., Ltd.

[0045]

[0046] Wherein, HFT is a human heavy chain of human ferrtin, and PFT is a heavy chain of human ferrtin from pyrococcus furiosus.

[0047] GFP-intein ...

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Abstract

The invention discloses a nano-preparation capable of simultaneously delivering an antigen and an immunoenhancer. The nano-preparation is capable of efficiently simultaneously delivering the antigen and the immunoenhancer and has good specificity. The nano-preparation is nanoparticles formed by 24 polymers self-assembled by a recombinant protein gbl-inteinc-ferrtin formed by fusion of the N-terminal of ferritin and the C-terminal of a connector gbl-intein; the C-terminal of the exogenous protein is fused with the N-terminal of the intein to form the recombinant protein, the N-terminal of the intein specificaly recognizes and excises ferritin exposed to the surface of the nanoparticles; the covalent cross-linking of the exogenous protein with ferritin is performed to obtain the product, wherein the exogenous protein is an immunoenhancer or antigen or a combination of the immunoenhancer and the antigen.

Description

technical field [0001] The invention relates to the field of nanomedicine, in particular to a nano preparation capable of simultaneously delivering antigens and immune enhancers. Background technique [0002] Vaccines are the most effective and economical medical interventions to prevent and control infectious diseases. Attenuated or inactivated pathogens have achieved great success as traditional classic vaccines, helping humans eliminate smallpox and polio. However, traditional vaccines have safety hazards and cannot effectively prevent viruses with high-frequency mutation characteristics, such as HIV and influenza viruses. [0003] Subunit vaccines have attracted the attention of vaccinologists because of their advantages such as easy purification, safety and efficacy, and stable physical and chemical properties. Subunit vaccines have poor immunogenicity, weak and short duration of induced immune response, which limits their clinical application. A new generation of va...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/64A61K39/385A61K39/39A61K39/00A61P35/00
CPCA61K39/00A61K39/385A61K39/39A61K47/644A61K2039/55516A61K2039/575A61K2039/6031A61P35/00
Inventor 汤书兵周伟袁伟明
Owner GUANGZHOU WOMEN AND CHILDRENS MEDICAL CENTER
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