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Short-disinfection-time spathiphyllum stem tip tissue culture and rapid propagation method

A technique of tissue culture, rapid propagation and shoot tip, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of high difficulty in surface disinfection, affecting proliferation and seedling quality, shortening disinfection time, and improving seedling quality. Seedling quality, rapid growth recovery effect

Active Publication Date: 2019-09-06
广州花卉研究中心
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] There are many domestic reports on tissue culture and rapid propagation of Phyllostachys chinensis, but leaves, petioles, terminal buds, stems, pedicels, inflorescences and other tissues and organs are usually used as explants, and the surface disinfection is difficult, and the pollution rate is between 10% to 87%, and after multi-generation culture, it is easy to produce endophytic bacteria, which will affect the subsequent proliferation and seedling quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] 1. Selection and disinfection of explants

[0027] Select the biennial Meijiuba which grows vigorously in the greenhouse and is free from diseases and insect pests. Cut the plants in August, cut off the whole plant, take the stem section with 4 to 5 petioles about 5cm, wash the stem section, and dry it in the air. Use 0.6% (6mg / mL) chlorine-containing disinfection solution (prepared with Junhong brand disinfection powder, its effective chlorine content is 18-21%, w / w) to shake and clean for 40 minutes, change the cleaning solution once during the period, and rinse with deionized water Clean, peel off 2 petioles under sterile conditions, add 0.1% (ie 1mg / mL) HgCl 2 The solution was soaked and disinfected for 4 minutes, rinsed with sterilized deionized water for 6 times, blotted dry, and the stem tips with 1 to 2 leaf primordia and 1 to 2 mm in diameter were removed as explants. The success rate of stem tip disinfection reached 96.7%.

[0028] 2. Shoot tip culture

[0...

Embodiment 2

[0037]1. Explant selection and disinfection

[0038] Select the biennial Meijiuba which grows vigorously in the greenhouse and is free from diseases and insect pests. Cut the plant in October, cut off the whole plant, take the stem section with 4 to 5 petioles about 5 cm, wash the stem section, and dry it in the air. Use 0.6% (ie 6mg / mL) chlorine-containing disinfection solution (prepared with Junhong brand disinfection powder, its available chlorine content is 18-21%, w / w) to shake and clean for 40 minutes. Change the cleaning solution once during the period, rinse with deionized water, peel off 2 petioles under aseptic conditions, and add 0.1% (ie 1mg / mL) HgCl 2 The solution was soaked and disinfected for 8 minutes, rinsed with sterilized deionized water 6 times for about 35 minutes, blotted dry, and stripped the stem tip with 1 to 2 leaf primordia and 1 to 2 mm in diameter as explants. The success rate of stem tip disinfection reaches 100%.

[0039] 2. Shoot tip culture ...

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Abstract

The invention discloses a short-disinfection-time spathiphyllum stem tip tissue culture and rapid propagation method which includes the steps: 1) explant selection and disinfection; 2) stem tip culture; 3) cluster bud induction; 4) cluster bud proliferation; 5) rooting and transplanting. According to the method, a stem tip with 1-2 pieces of leaf primordia and the diameter of 1-2mm serves as an explant, disinfection time of 0.1% liter of mercury can be greatly shortened, injure of heavy metal to plants is furthest reduced, disinfected stem tips completely survive and rapidly grow and restore,and mutation risks caused by toxicity of the heavy metal are reduced. According to the method, culture and proliferation are implemented by the aid of cytokinin (0.6-1mg / L 6-BA) with relatively lowerconcentration, higher proliferation rate is acquired, the mutation probability in the proliferation process is reduced, seedling quality is improved, rooted plants are transplanted, and the survival rate of the plants reaches 99% or more.

Description

technical field [0001] The invention belongs to the field of plant seedling production, and in particular relates to a method for tissue culture and rapid propagation of the stem tip of Atractylodes spp. with a short disinfection time. Background technique [0002] Spathiphyllum is a species of Spathiphyllum in the Araceae family. Its flowers and leaves have high ornamental value, and the plant shape is beautiful, moderate in size, shade-tolerant, and has the effect of purifying indoor air. It is suitable for home and office placement. It is deeply loved by consumers and has a broad market prospect. [0003] Sclerophyllum is usually propagated by division, which has a low reproduction coefficient, long cycle, and is easy to carry viruses, which greatly limits the large-scale and commercial production of Sclerophyllum. However, through plant tissue culture, a large number of high-quality tissue culture seedlings can be produced in a short period of time to meet market demand...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008A01H4/005
Inventor 吴贤彬周晓云宿庆连黄明翅曾伟达易懋升冯肖梅
Owner 广州花卉研究中心
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