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Method for transplanting passiflora edulis tissue cultured seedlings

A passionflower group and culture medium technology, applied in the directions of botanical equipment and methods, cultivation, soilless cultivation, etc., can solve the root damage of the root system, reduce the root function of the plant, reduce the survival rate of tissue culture seedling transplantation, and affect the passionflower seedling. Growth and other problems, to improve the transplant survival rate, easy to survive and grow, and improve labor efficiency.

Inactive Publication Date: 2016-04-27
广西壮族自治区农业科学院生物技术研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

During the transplanting process of passionflower tissue culture seedlings, due to transplanting from sterile culture medium to bacteria-carrying planting substrate, because the substrate is not clean, bacteria are carried to varying degrees, which can easily cause root pathogens to cause rotten roots and seedlings and reduce the risk of infection. Transplanting survival rate of tissue culture seedlings; the traditional method needs to wash the seedlings to remove the medium before transplanting, which will cause most of the root damage and cause abnormal root function of the plant, which will affect the growth of passionflower seedlings; the traditional cultivation medium generally uses soil, Its air permeability and water retention are poor, and it is difficult to provide proper water and nutrition for passionflower seedlings, thereby affecting the growth of passionflower seedlings

Method used

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  • Method for transplanting passiflora edulis tissue cultured seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] A kind of transplanting method of passionflower tissue culture seedling, comprises the following steps:

[0025] (1) take out the cultivated Passiflora tissue cultured seedlings into clumps from the culture container, place them in the bottom and hollowed out containers around the bottom, the container is a water-permeable basket, and 30 clumps are adorned in every basket; Cultivating seedlings does not remove the medium of the roots;

[0026] (2) the root of the Passiflora tissue culture seedling in the whole container is fully immersed in the disinfectant solution, and the immersion time is 1min, and then taken out; the disinfectant solution contains the following components by mass percentage: 30% carbendazim, agricultural 40% streptomycin and 30% hymexazol; the dilution factor of the disinfectant solution is 1000 times when used;

[0027] (3) The passionflower tissue culture seedlings that take out again become clump colonization in the culture medium; Described cu...

Embodiment 2

[0029] A kind of transplanting method of passionflower tissue culture seedling, comprises the following steps:

[0030] (1) take out the cultivating passionflower tissue cultured seedlings into clumps from the culture container, put them in the bottom and around the hollow container, the container is a water-permeable basket, and every basket is filled with 35 clumps; the passionflower group that is taken out Cultivating seedlings does not remove the medium of the roots;

[0031] (2) the root of the Passiflora tissue culture seedling in the whole container is fully immersed in the disinfectant solution, and the immersion time is 10min, and then taken out; the disinfectant solution contains the following components by mass percentage: 40% carbendazim, agricultural 20% streptomycin and 40% hymexazol; the dilution factor of the disinfectant is 2000 times when used;

[0032] (3) The passionflower tissue culture seedlings that take out again become clump colonization in the cultur...

Embodiment 3

[0034] A kind of transplanting method of passionflower tissue culture seedling, comprises the following steps:

[0035] (1) take out the cultivating passionflower tissue cultured seedlings into clumps from the culture container, put them in the bottom and hollowed out containers around the bottom, the container is a water-permeable basket, and every basket is filled with 25 clumps; the passionflower group that is taken out Cultivating seedlings does not remove the medium of the roots;

[0036] (2) the root of the Passiflora tissue culture seedling in the whole container is fully immersed in the disinfectant solution, and the immersion time is 5min, and then taken out; the disinfectant solution contains the following components by mass percentage: 35% carbendazim, agricultural 30% streptomycin and 35% hymexazol; the dilution factor of the disinfectant solution is 1500 times when used;

[0037] (3) The passionflower tissue culture seedlings that take out again become clump colo...

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Abstract

The invention belongs to the technical field of fruit tree cultivation, and particularly relates to a method for transplanting passiflora edulis tissue cultured seedlings. The method comprises the following steps of (1) taking out clusters of cultivated passiflora edulis tissue cultured seedlings from a culture vessel, placing the clusters in a container having a hollow bottom and four hollow sides, and determining the number of placed clusters according to the size of the container; (2) completely soaking roots of the passiflora edulis tissue cultured seedlings in the whole container in disinfectant for 1-10 minutes, and taking out the roots from the disinfectant; and (3) planting the clusters of the passiflora edulis tissue cultured seedlings in a culture medium. The method can be used for increasing the transplanting survival rate of the passiflora edulis tissue cultured seedlings, and the tissue cultured seedlings can quickly grow and recover; the disinfectant used in the method is reasonable in formula, can be used for reducing occurrence of damping-off pathogenic bacteria in the transplanting period of the passiflora edulis tissue cultured seedlings, and is beneficial to growth of roots of the transplanted passiflora edulis tissue cultured seedlings; and the culture medium used in the method has an effect of air permeability and water retention, and is beneficial to survival and growth of the passiflora edulis tissue cultured seedlings.

Description

technical field [0001] The invention belongs to the technical field of fruit tree cultivation, in particular to a method for transplanting passion flower tissue cultured seedlings. Background technique [0002] Passiflora edulia Sims, also known as passion fruit and egg fruit, is a herbaceous vine of the Passiflora family Passiflora, about 6 meters long; the stem has thin stripes and is glabrous; 5 petals, with sepals, etc. Long; base light green, middle purple, top white, berry ovoid, 3-4 cm in diameter, glabrous, purple when ripe; many seeds, ovate. Flowering in June, fruiting in November. [0003] Passion fruit can be eaten raw or used as vegetables and fodder. Being used as medicine has the effect of excitement and strength. The fruit pulp is juicy, adding bicarbonate and sugar can be made into an aromatic and delicious drink, and it can also be added to other drinks to improve the quality of the drink. The seeds are pressed for oil, which can be used for food, soap ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G31/00A01C1/00
CPCA01G31/00A01C1/00
Inventor 黄伟华刘洁云牟海飞韦绍龙黄永才吴艳艳吴代东周天盛周承辉
Owner 广西壮族自治区农业科学院生物技术研究所
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