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Drug targets for treating colon cancer

A colon cancer, drug technology, applied in the field of biomedicine, can solve problems such as increasing public health expenditure and financial burden, affecting individual families, and social impact.

Active Publication Date: 2019-08-30
XUZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Tumor occurrence not only affects individual families, but also brings serious social impact, increasing public health expenditure and financial burden

Method used

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  • Drug targets for treating colon cancer
  • Drug targets for treating colon cancer
  • Drug targets for treating colon cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0056] Example 1 Study on the regulatory effect of PTBP3 on the expression of HIF-1α and its downstream genes

[0057] 1. Cell culture

[0058] Inoculate HCT116, SW480, and SW620 cells in DMEM complete medium (add 10% FBS, 100 U / ml penicillin, 100 μg / ml streptomycin), and place them in 5% CO 2 , 37 ℃ incubator culture. By adding 94% N 2 / 5%CO 2 The mixed gas is pumped into the incubator to generate 1% O 2 cultivation environment.

[0059] 2. Western blot

[0060] According to the previously described method (references: Hou P, Li L, Chen F, Chen Y, Liu H, Li J, et al. PTBP3-Mediated Regulation of ZEB1 mRNA Stability Promotes Epithelial-Mesenchymal Transition in Breast Cancer. Cancer research 2018; 78 : 387-398) for Western blot.

[0061] 3. Construction of stable transfected cell lines

[0062] Follow the previously described method (references: Hou P, Li L, Chen F, Chen Y, Liu H, Li J, et al. PTBP3-Mediated Regulation of ZEB1 mRNA Stability Promotes Epithelial-Mesench...

Embodiment 2

[0079] Example 2 Regulatory Effects of PTBP3 and HIF-1α on Tumor Cell Proliferation, Migration and Invasion

[0080] 1. Cell migration and invasion

[0081] Cells were starved for 24 h in serum-free medium and seeded in the upper chamber of a 24-well transwell chamber (8.0 μm, Corning, NY, USA.). For invasion experiments, the upper chamber was also coated with Matrigel (BD Biosciences). Add complete medium to the lower chamber to stimulate cell migration or invasion. After incubation for 24-48h, cells adhered to the lower surface of the membrane were stained with 0.1% crystal violet, and the number of migrated or invaded cells was counted. Five random fields of view were taken under the microscope for counting.

[0082] 2. Cell proliferation

[0083] Use the CCK-8 method to detect cell proliferation, inoculate an equal amount of cells in a 96-well plate, add 10 μL of CCK8 reagent to each well after 48 hours, and use a microplate reader to detect the absorbance at 450 nm, w...

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Abstract

The invention discloses drug targets for treating colon cancer. The drug targets are PTBP3 and HIF-1alpha. The invention takes in-vitro stable transfection cell lines or xenografts formed by the sameas research objects, and proves that the inhibition of HIF-1alpha can enhance the inhibition of PTBP3 through the drug synergistic effect so as to realize the treatment effect on colon cancer. The twodrug targets are simultaneously inhibited to have obvious drug synergistic anti-tumor effect, and can be used as a novel treatment method for colon cancer.

Description

technical field [0001] The invention belongs to the field of biomedical research and relates to a drug target for treating colon cancer, in particular to the application of PTBP3 and / or HIF-1α in the preparation of drugs for treating colon cancer. Background technique [0002] Colon cancer is one of the common malignant tumors, with the highest incidence in the 40-50 age group. According to the world epidemiological survey, colon cancer has the highest incidence rate in North America, Western Europe, Australia, New Zealand and other places, ranking the top two in visceral tumors, and its incidence and mortality in China are lower than those of common malignancies such as gastric cancer, esophageal cancer, and lung cancer. tumor. In recent years, data from various places have shown that along with the improvement of people's living standards and the change of diet structure, its incidence rate is on the rise year by year. [0003] Tumor occurrence not only affects individua...

Claims

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Application Information

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IPC IPC(8): A61K45/00A61K45/06A61P35/00
CPCA61K45/00A61K45/06A61P35/00
Inventor 侯平甫白津郑骏年陈芳李敏乐李中伟
Owner XUZHOU MEDICAL UNIV
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