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A kind of directional culture medium and directional culture seedling-forming method of germinated seeds of orchid

A technology for germinating seeds and a culture medium, which is applied to the directional medium for germinating seeds and the field of directional culture to grow seedlings, so as to achieve the effects of improving seedling efficiency and shortening seedling time.

Active Publication Date: 2021-01-29
INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no technology that adopts the germinated seeds of orchid as explants to carry out directional culture into seedlings

Method used

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  • A kind of directional culture medium and directional culture seedling-forming method of germinated seeds of orchid
  • A kind of directional culture medium and directional culture seedling-forming method of germinated seeds of orchid
  • A kind of directional culture medium and directional culture seedling-forming method of germinated seeds of orchid

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Experimental program
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Effect test

Embodiment 1

[0041] 1 Test materials and methods

[0042] 1.1 Test material

[0043] The germinated seeds that can be distinguished by naked eyes are used as explants (about 1*1*1mm) for the protocorm proliferation test, the proliferated protocorms are used as explants for bulb differentiation, and the corms produced by the differentiation of protocorms are used as explants for rooting test .

[0044] 1.2 Effect of mitogens on protocorm proliferation

[0045]MS+NAA0.1mg / L+sucrose 30g / L+agar powder 4.5g / L was used as the basic medium, and mitogens were added as TDZ, Zt, 6-BA, and KT, and the addition amounts were 0.1mg / L and 2.0mg / L , 4.0mg / L, 6.0mg / L, 8.0mg / L, 10.0mg / L, the culture temperature was 23°C, the light-dark cycle was 14h / 10h, the light intensity was 1500lx, and the culture time was 55d. Each treatment was repeated 3 times. Repeat 15 explants, and the statistical index is the average weight of the explants.

[0046] 1.3 Effect of auxin on protocorm proliferation

[0047] MS+...

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Abstract

The invention relates to the technical field of tissue culture, in particular to a directional culture medium for germinated orchid seeds and a method for directional cultivation of seedlings. The directional medium includes, proliferation medium: MS medium containing sucrose, agar powder, 6-BA, NAA; differentiation medium: MS medium containing sucrose, agar powder, KT, IBA; rooting medium: containing sucrose , agar powder, 6-BA, IAA MS medium. In this application, the germinated seeds of Shanlan were used as test materials to study the regulation and promotion of different mitogens and auxins and their ratios on the process of Shanlan from germination to seedling development. The results show that 6-BA and NAA are beneficial to protocorms. Proliferation, KT and IBA are conducive to the differentiation of protocorms into corms, and 6‑BA and IAA are conducive to corm rooting. The method of the invention enables the orchid germinated seeds to develop directionally, shortens the time for seedling formation, and improves the efficiency of seedling formation.

Description

technical field [0001] The invention relates to the technical field of tissue culture, in particular to a directional culture medium for germinated orchid seeds and a method for directional cultivation of seedlings. Background technique [0002] Oreorchis patens (Lindl.) Lindl. is a perennial shade herbaceous plant of Orchidaceae Oreorchis. It is widely distributed in Southwest my country, Central China, Northwest China and Northeast China. It also has a small amount of distribution in the Korean Peninsula, Siberia and Japan. Its dried corm is used as medicine, sweet, pungent, cold, slightly poisonous. It has the effects of nourishing yin and clearing the lung, resolving phlegm and relieving cough, and is used for carbuncle rickets, sores, scrofula, and unknown swelling. However, the medicinal value of Shanlan has not been valued for a long time, but it has been treated as a counterfeit of Shanzi mushroom or used as a customary medicine. [0003] In recent years, with the ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N5/04A01H4/00
CPCA01H4/001A01H4/008
Inventor 张正海张亚玉孙海张悦
Owner INST OF SPECIAL ANIMAL & PLANT SCI OF CAAS
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