Multi-dimensional liquid chromatography separation system based on two-position eight-way valve

Abstract

The invention provides a multi-dimensional liquid chromatography separation system comprising a high performance liquid chromatography gradient pump A, a high performance liquid chromatography gradient pump B, a high performance liquid phase dilution liquid pump, a gradient mixer A, a gradient mixer B, a sampling valve, an enriched column array A, an enriched column array B, a distillate collector, a liquid chromatography column array, a detector, a two-position eight-way valve and a connecting pipeline. Conversion from the previous dimensional separation state to the next dimensional separation state is realized by the two-position eight-way valve to achieve three or more-dimensional chromatographic separation. Through the liquid chromatography separation column array, each-dimensional chromatographic separation column is selected, thereby, based on the same gradient elution system and the same detector, realizing the all on-line monitoring and control of multi-dimensional chromatographic separation and realizing controllable degree of cleanliness of the enrichment columns and separation columns. Each-dimensional separation is connected by an enrichment column and a diluent pump is used to assist in compound enrichment or capture. The multi-dimensional liquid chromatography separation system provided by the invention achieves efficient separation of monomer compounds in complex system samples with high separation difficulty by selecting different combinations of chromatographic stationary phases and mobile phases.

Description

technical field [0001] The invention belongs to the technical field of high performance liquid chromatography separation, and relates to a multidimensional liquid chromatography separation system. Background technique [0002] With the development of separation technology, exploring and separating components in complex sample systems has become a hot research field. Multidimensional liquid chromatography technology can effectively improve the resolution of complex sample component separation by increasing peak capacity, and has become the development direction of fast chromatography separation technology. Multidimensional liquid chromatography technology is a liquid chromatography technology that sequentially injects the eluent of the first-dimensional chromatographic column of the sample into the subsequent dimensional chromatographic column for further separation. This separation technology can utilize two or more chromatographic columns with different separation mechanis...

AI Technical Summary

Problems solved by technology

The serial mode multidimensional liquid chromatography system is represented by the sepbox series products of the fully automatic high-throughput preparative separation system of Sepiatec GmbH in Germany, but this product can only perform two-dimensional chromatographic separation, and the separation capacity is limited

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