Microfluid control system for separating, focusing and sorting of rare cells and application

Abstract

The invention relates to the technical field of detection of rare cells, in particular to a microfluid control system for separating, focusing and sorting of rare cells and application. A microfluid control chip device comprising a separating unit, a focusing unit, a sorting unit and a flow resistance matching unit serves as the core of the system. The system is characterized in that the system can integrate operation like separation, focusing, single-cell analysis and sorting on the rare cells in body fluid such as blood in a continuous mode on one chip for completion, and therefore, the system can be widely applied to separation and sorting of the rare cells in the body fluid, and disease diagnosis, drug screening, individual-based treatment and other fields based on the rare cells.

Description

technical field [0001] The invention relates to the technical field of detection of rare cells, in particular to a microfluidic system and application for separation, focusing and sorting of rare cells. Background technique [0002] The information disclosed in the Background of the Invention is only intended to increase the understanding of the general background of the invention, and is not necessarily to be taken as an acknowledgment or any form of suggestion that the information constitutes the prior art that is already known to those skilled in the art. [0003] Human blood and other body fluids contain some rare cells that are rare but have important clinical value, such as circulating tumor cells (CTCs), fetal nucleated red blood cells (Fetal NRBCs), and virus-infected or parasitic cells. These rare cells can directly or indirectly reflect the health status of the body. Among them, CTCs called "liquid biopsy" are of great significance for the study of cancer stage dia...

AI Technical Summary

Problems solved by technology

The inventors believe that although these methods represent important advances in this field, their technical bottlenecks still exist; first, the single antibody capture that currently relies on the epithelial marker EpCAM causes epithelial-derived missing CTCs to fail to be captured, thereby cause missed detection

Second, the release of CTCs based on antibody capture is difficult, which is not conducive to the high-throughput sorting and subsequent analysis of CTCs.

Thirdly, although separation methods based on physical properties are relatively simple to operate, have high throughput, and are not dependent on the expression of cell surface markers, they are limited by the heterogeneity of CTCs in physical properties, and such methods often lose small-sized CTCs. resulting in incomplete separation and low purity

Finally, the current CTCs detection method with independent separation and analysis technologies not only cannot integrate the separation, focusing, single-cell analysis and sorting of rare cells in body fluids into a single chip, but also can be used in cell transfer, container replacement, etc. Such links can easily cause sample loss and contamination, resulting in large system errors

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