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Fermentation reactor for preparing citric acid from immobilized Aspergillus niger and a method thereof

A reactor, the technology of Aspergillus niger, which is applied in the field of fermentation reactors for immobilizing Aspergillus niger to produce citric acid, can solve the problems of unfavorable separation and purification of products in the later stage of fermentation, limited number of times of repeated use of carriers, and expansion of industrial-scale production, etc. The effect of mass and heat transfer performance, complete consumption of residual sugar, and shortening of fermentation period

Pending Publication Date: 2019-08-23
NANJING HIGH TECH UNIV BIOLOGICAL TECH RES INST CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, in the existing immobilization process, the carrier preparation process is relatively complicated, the loss of carrier recovery is large, the number of carrier reuse is limited, and the use of some carriers is not conducive to the separation and purification of late fermentation products, so it is difficult to produce on an industrial scale.

Method used

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  • Fermentation reactor for preparing citric acid from immobilized Aspergillus niger and a method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0035] Immobilized culture, fermentation after embodiment 1 Aspergillus niger spore expansion

[0036] Liquefaction sugar production: 11kg corn flour is mixed with 33 liters of water, the pH is 5.8-6.0, add liquefaction enzyme 16U / g corn flour, liquefy at 65°C for 45 minutes, heat up to 95°C for 45 minutes, and DE reaches 25%. Keep 8L of the returned material with slag for later use, and the remaining liquefied mash is subjected to centrifugation, and the supernatant liquid is for later use.

[0037]Seed preparation: Prepare 5L of seed solution with slag-returned material (using 10L seed tank, T&J-Btype×2, DBeer Bioengineering (Shanghai) Co., Ltd.), total sugar 120g / L, inoculated spores 1g, ventilation volume 400L / h, the rotation speed is 500r / min, the temperature is 36°C and cultivated for about 26 hours, the pH is below 2.5, the acidity is 1.5-2.0, and the bacteria balls are uniform.

[0038] Immobilized culture and fermentation: after the seed culture is finished, insert ...

Embodiment 2

[0039] Carry out immobilized culture, fermentation after embodiment 2 Aspergillus niger spore expansion culture

[0040] Insert the cultured spores into an immobilized fermenter equipped with a fermentation medium at a rate of 0.2g / L (the immobilized carrier is porous polyurethane). The first batch is equivalent to the fixed culture process, and the preparation of the fermentation medium and the culture conditions are the same as the implementation Example 1, discharge the fermentation broth until the reducing sugar drops below 1g / L, and add new fermentation medium again. 12 batches are run continuously, the average fermentation time is 46 hours, and the average sugar-acid conversion rate reaches 95.5%.

Embodiment 3

[0041] The influence of embodiment 3 different carrier sizes on immobilization and fermentation

[0042] Insert 0.2g / L of the cultured spores into an immobilized fermenter equipped with a fermentation medium (the carrier is activated carbon filter cotton), and investigate different sizes (1cm×1cm×1cm, 2cm×2cm×1cm, 2.5cm× 2.5cm * 2.5cm) on Aspergillus niger immobilization and fermentation influence, each fermentation 3 batches (the first batch is equal to the fixed culture process, does not count in fermentation batch), fermentation medium and culture condition are with embodiment 1. The results showed that the second fermentation had the best effect, and the acid production rates were 3.16g / L / h, 3.33g / L / h, and 2.85g / L / h, respectively.

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Abstract

The invention discloses a fermentation reactor for preparing citric acid from immobilized Aspergillus niger and a method thereof. The reactor comprises a fermentation tank. A carrier frame is arrangedin the middle of the fermentation tank. The carrier frame is filled with an immobilized carrier; a cylinder longitudinally penetrates through the middle of the carrier frame, a push type paddle is arranged inside the upper end of the cylinder, a turbine paddle and a horizontal paddle are sequentially arranged below the cylinder, and the turbine paddle and the horizontal paddle are located below the carrier frame; the push type paddle, the turbine paddle and the horizontal paddle are distributed on the same rotating shaft from top to bottom, the top of the rotating shaft is connected with a motor above the fermentation tank, and the motor drives the push type paddle, the turbine paddle and the horizontal paddle to rotate at the same time; a gas distributor is arranged at the bottom of thefermentation tank, and the gas distributor is located below the horizontal paddle. The fermentation reactor is good in fermentation stability and capable of repeatedly fermenting acid in batches, keeping the yield stable and not lowering the acid producing level, the final citric acid yield can reach 180 g / L or above, the fermentation rate reaches 3.1 g / L / h or above, and the sugar acid conversionrate reaches 97% or above.

Description

technical field [0001] The invention belongs to the technical field of industrial biology, and in particular relates to a fermentation reactor for producing citric acid by immobilized Aspergillus niger and a method thereof. Background technique [0002] As one of the organic acids with the largest demand in the world, citric acid is widely used in food, chemical, pharmaceutical and other industries. At present, my country's annual output of citric acid is more than 2 million tons, accounting for more than 80% of the world's total, and it is the world's largest producer and exporter of citric acid. The traditional citric acid industry has problems such as time-consuming seed preparation and low production efficiency, which seriously restrict the development of the industry. [0003] The surface-immobilized continuous fermentation system of Aspergillus niger can solve the problems of many by-products, poor tolerance, and low conversion rate, but the interface interaction mech...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/16C12M1/04C12M1/02C12M1/00C12P7/48C12R1/685
CPCC12M21/16C12M33/04C12M27/02C12P7/48
Inventor 应汉杰陈勇刘庆国余斌赵南邹亚男
Owner NANJING HIGH TECH UNIV BIOLOGICAL TECH RES INST CO LTD
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