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Method for preparing safflower extract through eutectic solvent

A technology of deep eutectic solvent and safflower extract, which is applied to medical preparations containing active ingredients, pharmaceutical formulas, plant raw materials, etc., can solve problems such as difficult quality control, low efficiency, and easy deterioration, and avoid extraction time long effect

Inactive Publication Date: 2019-08-23
ZHEJIANG CHINESE MEDICAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

With the continuous advancement of the modernization of traditional Chinese medicine in my country, problems such as low efficiency, poor repeatability, and narrow polarity range of traditional extraction methods have attracted more and more attention.
Specifically for the extraction and preparation of safflower, it is characterized by low extraction efficiency of water extract, difficult quality control, and easy deterioration.

Method used

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  • Method for preparing safflower extract through eutectic solvent
  • Method for preparing safflower extract through eutectic solvent
  • Method for preparing safflower extract through eutectic solvent

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Embodiment 1.DES-17 is a solvent

[0028] Mix L-proline (1.15g), acetamide (0.59g), and deionized water (36μL) at a molar ratio of 1:1:2, heat and stir at 80°C for 1 hour to form a clear and uniform low-ethanol Melt solvent, recorded as DES-17. Mix 750μL of DES-17 and 250μL of water evenly, add it to a 1.5mL centrifuge tube, then accurately weigh 200.0mg of safflower powder and add it, and extract by ultrasonic at 50°C for 30min with an ultrasonic power of 200W. After ultrasonic extraction, centrifuge at 13300r / min for 10min, and take the supernatant, which is the safflower extract. The supernatant was diluted 10 times with volume concentration 50% aqueous methanol solution and then detected by high performance liquid chromatography ( figure 1 ), according to the compound (hydroxysafflower yellow A and hydroxysafflower yellow A) standard curve, obtain the type and content of the compound contained in the safflower extract, and calculate the extraction with the extract...

Embodiment 2D

[0030] Embodiment 2DES-21 is solvent

[0031] Choline chloride (1.40g), acetamide (0.59g), and deionized water (18μL) were mixed at a molar ratio of 1:1:1, heated and stirred at 80°C for 1 hour to form a clear and uniform eutectic Solvent, recorded as DES-21. Mix 750 μL of DES-21 and 250 μL of water evenly, add it to a 1.5 mL centrifuge tube, then accurately weigh 200.0 mg of safflower powder and add it to it, and perform ultrasonic extraction at 50°C for 30 min with an ultrasonic power of 200W. After ultrasonic extraction, centrifuge at 13300r / min for 10min, and take the supernatant, which is the safflower extract. After the supernatant is diluted 10 times with volume concentration 50% aqueous methanol solution, it is detected by high-performance liquid chromatography described in Example 1 ( figure 2 ), the measured extract contains: hydroxy safflower yellow A, 29.6mg / g; dehydrated safflower yellow B, 13.7mg / g.

Embodiment 3

[0032] Embodiment 3.DES-10 is solvent

[0033] Mix L-proline (1.15g) and lactic acid (1.80g) at a molar ratio of 1:1, heat and stir at 80°C for 1 hour to form a clear and uniform deep eutectic solvent, which is designated as DES-10. Mix 750 μL of DES-10 and 250 μL of water evenly, add it to a 1.5 mL centrifuge tube, then accurately weigh 200.0 mg of safflower powder and add it, and extract it by ultrasonic at 50°C for 30 min, with an ultrasonic power of 200W. After ultrasonic extraction, centrifuge at 13300r / min for 10min, and take the supernatant, which is the safflower extract. After the supernatant is diluted 10 times with volume concentration 50% aqueous methanol solution, it is detected by high-performance liquid chromatography described in Example 1 ( image 3 ), the measured extract contains: hydroxy safflower yellow A, 30.3mg / g; dehydrated safflower yellow B, 13.5mg / g.

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Abstract

The invention discloses a method for preparing a safflower extract through a eutectic solvent. The method comprises the steps of adding safflower powder into an aqueous solution of the eutectic solvent, performing ultrasonic extraction for 15-60 minutes at the temperature of 25-75 DEG C under the power of 50-200 W, performing centrifuging, and taking a supernatant to obtain the safflower extract.According to the efficient and green method for preparing the safflower extract through the eutectic solvent, main components in safflower can be simultaneously extracted efficiently, wherein the content of dehydrated safflower yellow B is 45.9% higher than that of an aqueous extract. The solvent dosage in the method is low, wherein extraction of 200 mg of a safflower medicinal material requires 1mL of the extraction solvent; the defects are effectively avoided that the extraction time required by other solvents is low and extracts are likely to go bad; therefore, the method is regarded as anovel extraction method suitable for Chinese medicine and other natural medicine.

Description

[0001] (1) Technical field [0002] The invention relates to a method for preparing safflower extract by using a deep eutectic solvent. [0003] (2) Background technology [0004] Safflower is the dried flower of Carthamus tinctorius L, a plant of the genus Safflower in the Compositae family, and is widely used in traditional Chinese medicine in my country. After years of clinical and pharmacological research, it has been found that safflower has certain effects on the cardiovascular and cerebrovascular systems, the nervous system, and the immune system. , bruises, joint pain, coronary heart disease, angina pectoris and other diseases, common Chinese patent medicines such as Danhong injection and Guhong injection all use safflower as the main medicinal flavor. [0005] The main active ingredients of safflower are total flavonoids and safflower yellow compounds. The chemical composition structure of safflower includes flavonoids, alkaloids, sesquiterpenes, organic acids, polya...

Claims

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Application Information

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IPC IPC(8): A61K36/286A61K133/00
CPCA61K36/286A61K2236/333
Inventor 万浩宇石文钦李畅万海同
Owner ZHEJIANG CHINESE MEDICAL UNIVERSITY
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