Infected mouse model for lung delivery through liquid aerosol with plague bacilli

Abstract

The invention discloses an infected mouse model for lung delivery through liquid aerosol with plague bacilli and provides a method for preparing an animal model. The method comprises the following steps that pathogenic bacteria are delivered to the animal lung through a liquid aerosol mode, and the animal model is obtained. The pathogenic bacteria are plague bacilli; or the animal is a mouse. According to the model, a test sample is directly delivered to the mouse lung through the liquid aerosol mode, a novel pulmonary plague mouse infection model is established, the model is high in targetingperformance and good in repeatability, and the infection condition of the test sample on the mouse can be accurately and quantitatively researched.

Description

technical field [0001] The invention relates to a method for preparing a mouse infection model, in particular to a method for constructing a mouse model infected with pathogenic bacteria delivered by liquid aerosol lungs. Background technique [0002] Plague is an ancient natural foci disease, which has caused three worldwide pandemics in history and claimed the lives of hundreds of millions of people. Yersinia pestis is one of the bacteria in the genus Yersinia pestis that can cause serious human infections. People may be infected by contact with the blood and tissues of infected animals, or exposed to bacterial aerosols. The manifestations are lymph glands Swelling, sepsis, or pneumonic plague, the latter two of which are highly contagious and often fatal if not treated with antibiotics within 24 hours of onset of symptoms. Plague infection is still a serious threat to public health in many countries and regions. The establishment of animal models of Y. pestis is insepar...

AI Technical Summary

Problems solved by technology

[0003] At present, most studies on liquid aerosol or dry powder aerosol use oral and nasal exposure and whole body exposure. Although these two methods are non-invasive to experimental animals, they cannot accurately quantify the dose, and the inhalation dose of the tested animals varies greatly.

Moreover, since the sample aerosol needs to fill the exposure tower or exposure chamber with a large capacity and reach a certain concentration, the demand for samples is large and the loss is also large, which increases the burden of biosafety protection

In addition, related instruments and equipment are bulky and expensive

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