Time-resolved fluorescence immunochromatographic reagent strip for rapid detection of cyclosporine
A time-resolved fluorescence, cyclosporine technology, applied in the field of medical testing, can solve the problems of requiring expensive equipment, expensive reagents, long detection time, etc., and achieves good commercial application prospects, reliable measurement results, and easy operation. Effect
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Embodiment 1
[0026] Refer to attached figure 1 , a time-resolved fluorescent immunochromatographic reagent strip for rapid detection of cyclosporine, which includes a detection card and an optional fluorescent immunoassay analyzer, the detection card includes a sample pad 1, a binding pad 2, and a detection line 3 and the detection pad 5, the absorption pad 6 and the backing plate 7 of the quality control line 4, the bonding pad 2 is sprayed with nano-microsphere time-resolved fluorescence-cyclosporine probe, and the nano-microsphere time-resolved fluorescence-cyclo The sporin probe is prepared by nano fluorescent microspheres and cyclosporine monoclonal antibody, the detection line 3 is prepared by spraying cyclosporine protein derivatives, and the quality control line 4 is prepared by spraying goat anti-mouse IgG antibody , the cyclosporine antibody is a murine antibody. The detection pad 5 is a nitrocellulose membrane and a porous structure membrane with a pore size of 5 μm; the materi...
Embodiment 2
[0037] Refer to attached figure 1 , a time-resolved fluorescent immunochromatographic reagent strip for rapid detection of cyclosporine, which includes a detection card and an optional fluorescent immunoassay analyzer, the detection card includes a sample pad 1, a binding pad 2, and a detection line 3 and the detection pad 5, the absorption pad 6 and the backing plate 7 of the quality control line 4, the bonding pad 2 is sprayed with nano-microsphere time-resolved fluorescence-cyclosporine probe, and the nano-microsphere time-resolved fluorescence-cyclo The sporin probe is prepared by nano fluorescent microspheres and cyclosporine monoclonal antibody, the detection line 3 is prepared by spraying cyclosporine protein derivatives, and the quality control line 4 is prepared by spraying goat anti-mouse IgG antibody , the cyclosporine antibody is a murine antibody. The detection pad 5 is a nitrocellulose membrane and a porous structure membrane with a pore size of 12 μm; the mater...
Embodiment 3
[0048] Refer to attached figure 1 , a time-resolved fluorescent immunochromatographic reagent strip for rapid detection of cyclosporine, which includes a detection card and an optional fluorescent immunoassay analyzer, the detection card includes a sample pad 1, a binding pad 2, and a detection line 3 and the detection pad 5, the absorption pad 6 and the backing plate 7 of the quality control line 4, the bonding pad 2 is sprayed with nano-microsphere time-resolved fluorescence-cyclosporine probe, and the nano-microsphere time-resolved fluorescence-cyclo The sporin probe is prepared by nano fluorescent microspheres and cyclosporine monoclonal antibody, the detection line 3 is prepared by spraying cyclosporine protein derivatives, and the quality control line 4 is prepared by spraying goat anti-mouse IgG antibody , the cyclosporine antibody is a murine antibody. The detection pad 5 is a nitrocellulose membrane and a porous structure membrane with a pore size of 7 μm; the materi...
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