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Time-resolved fluorescence immunochromatographic reagent strip for rapid detection of cyclosporine

A time-resolved fluorescence, cyclosporine technology, applied in the field of medical testing, can solve the problems of requiring expensive equipment, expensive reagents, long detection time, etc., and achieves good commercial application prospects, reliable measurement results, and easy operation. Effect

Pending Publication Date: 2019-08-16
SHANGHAI GENEXT MEDICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Among them, the HPLC-MS method is accurate and sensitive for the determination of blood drug concentration, but its operation is cumbersome, the detection time is long, the detection cost is high, and expensive equipment is required. It is mainly used in the field of scientific research or as a reference method
MEIA and CMIA are commonly used detection methods at present. Their detection is highly automated and the detection results are accurate, but the reagents are expensive and need to use supporting large instruments.
The enzyme-linked immunosorbent assay has the disadvantages of long detection time, complicated operation, and poor repeatability.

Method used

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  • Time-resolved fluorescence immunochromatographic reagent strip for rapid detection of cyclosporine

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Experimental program
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Effect test

Embodiment 1

[0026] Refer to attached figure 1 , a time-resolved fluorescent immunochromatographic reagent strip for rapid detection of cyclosporine, which includes a detection card and an optional fluorescent immunoassay analyzer, the detection card includes a sample pad 1, a binding pad 2, and a detection line 3 and the detection pad 5, the absorption pad 6 and the backing plate 7 of the quality control line 4, the bonding pad 2 is sprayed with nano-microsphere time-resolved fluorescence-cyclosporine probe, and the nano-microsphere time-resolved fluorescence-cyclo The sporin probe is prepared by nano fluorescent microspheres and cyclosporine monoclonal antibody, the detection line 3 is prepared by spraying cyclosporine protein derivatives, and the quality control line 4 is prepared by spraying goat anti-mouse IgG antibody , the cyclosporine antibody is a murine antibody. The detection pad 5 is a nitrocellulose membrane and a porous structure membrane with a pore size of 5 μm; the materi...

Embodiment 2

[0037] Refer to attached figure 1 , a time-resolved fluorescent immunochromatographic reagent strip for rapid detection of cyclosporine, which includes a detection card and an optional fluorescent immunoassay analyzer, the detection card includes a sample pad 1, a binding pad 2, and a detection line 3 and the detection pad 5, the absorption pad 6 and the backing plate 7 of the quality control line 4, the bonding pad 2 is sprayed with nano-microsphere time-resolved fluorescence-cyclosporine probe, and the nano-microsphere time-resolved fluorescence-cyclo The sporin probe is prepared by nano fluorescent microspheres and cyclosporine monoclonal antibody, the detection line 3 is prepared by spraying cyclosporine protein derivatives, and the quality control line 4 is prepared by spraying goat anti-mouse IgG antibody , the cyclosporine antibody is a murine antibody. The detection pad 5 is a nitrocellulose membrane and a porous structure membrane with a pore size of 12 μm; the mater...

Embodiment 3

[0048] Refer to attached figure 1 , a time-resolved fluorescent immunochromatographic reagent strip for rapid detection of cyclosporine, which includes a detection card and an optional fluorescent immunoassay analyzer, the detection card includes a sample pad 1, a binding pad 2, and a detection line 3 and the detection pad 5, the absorption pad 6 and the backing plate 7 of the quality control line 4, the bonding pad 2 is sprayed with nano-microsphere time-resolved fluorescence-cyclosporine probe, and the nano-microsphere time-resolved fluorescence-cyclo The sporin probe is prepared by nano fluorescent microspheres and cyclosporine monoclonal antibody, the detection line 3 is prepared by spraying cyclosporine protein derivatives, and the quality control line 4 is prepared by spraying goat anti-mouse IgG antibody , the cyclosporine antibody is a murine antibody. The detection pad 5 is a nitrocellulose membrane and a porous structure membrane with a pore size of 7 μm; the materi...

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Abstract

The invention provides a time-resolved fluorescence immunochromatographic reagent strip for rapid detection of cyclosporine. The reagent strip comprises a detection card and an optional fluorescence immunological quantitative analyzer, wherein the detection card comprises a sample pad, a bonding pad, a detection pad having a detection line and a quality control line, an absorption pad and a backing plate; a nano-microspheric time-resolved fluorescence-cyclosporin probe is sprayed on the bonding pad; the nano-microspheric time-resolved fluorescence-cyclosporin probe is prepared by nano fluorescent microspheres and a cyclosporine monoclonal antibody; the detection line is prepared by spraying of a cyclosporine protein derivative; the quality control line is prepared by spraying of a goat anti-mouse IgG antibody; and the cyclosporine antibody is a murine antibody. The time-resolved fluorescence immunochromatographic reagent strip for rapid detection of cyclosporine provided by the invention has high detection sensitivity, wide detection range, stable and reliable detection, simple and feasible operation, and has good commercial application prospects, and accurate and reliable measurement results can be obtained only in 5 minutes.

Description

technical field [0001] The invention relates to the technical field of medical testing, in particular to a time-resolved fluorescent immunochromatographic reagent strip for rapid detection of cyclosporine. Background technique [0002] Cyclosporine A (CsA), as an immunosuppressant, has played an important role in the treatment of transplant rejection and promoted the development of organ transplantation worldwide. Although the use of CsA is often accompanied by a variety of adverse reactions, there are still hundreds of thousands of patients worldwide using CsA to suppress rejection after transplantation. [0003] Because the absorption and metabolism of CsA in different patients often show great individual differences, even with the same drug dose, the blood concentration of CsA in different patients often varies significantly; due to the liver and kidney toxicity of CsA and its narrow therapeutic dose The efficacy and toxicity of CsA are closely related to the blood conce...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/558G01N33/543G01N33/533G01N21/64
CPCG01N33/558G01N33/54346G01N33/533G01N21/6428G01N21/6408
Inventor 刘斌虎吴云林傅琳
Owner SHANGHAI GENEXT MEDICAL TECH
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