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Fluorescent tracing method of Alpha-tricalcium phosphate hydration process

A tricalcium phosphate and fluorescent tracer technology, which is applied in the application field of bioactive fluorescent tracer technology, can solve problems such as inability to realize in-situ dynamic monitoring, affect clinical effects, and poor water resistance, and achieve continuous and stable luminescence performance, no Good biotoxicity and biocompatibility

Active Publication Date: 2019-08-16
SUZHOU DINGAN ELECTRONICS TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Clinical application shows that one of the main defects of CPC is poor water resistance
For CPC whose main component is α-TCP, it is easy to be eroded by plasma and body fluids during the hydration process of α-TCP and collapses, which not only affects the clinical effect, but more importantly, in percutaneous vertebral body formation, it is not hydrated. Once the α-TCP powder enters the cardiovascular system, it will cause thrombus and cause various complications, thus limiting the popularization and application of CPC
At present, the hydration process of α-TCP can only be detected indirectly on the X-ray diffraction analyzer after regular sampling, and in-situ dynamic monitoring cannot be realized.

Method used

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  • Fluorescent tracing method of Alpha-tricalcium phosphate hydration process
  • Fluorescent tracing method of Alpha-tricalcium phosphate hydration process
  • Fluorescent tracing method of Alpha-tricalcium phosphate hydration process

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Take 1 gram of alpha-Ca 2.97 Eu 0.03 (PO 4 ) 2 Powder immersed in 100ml 0.25M K 2 HPO 4 Hydrate in the buffer solution, the pH value of the buffer solution is 7.4, the temperature is 37±2°C, and the hydration time is 0~168h. α-Ca that will be hydrated for different times 2.97 Eu 0.03 (PO 4 ) 2 The powder was taken out from the buffer solution, washed three times with deionized water, and then dried in a constant temperature box at 70°C for 12 hours. Finally, the content of hydroxyapatite in the hydrate was analyzed by XRD, and the excitation spectrum was detected by PL. , calculate the CIE color coordinates of its spectrum or / and take photos of fluorescence excited by 365nm ultraviolet light.

[0030] See attached figure 1 , which is the α-Ca prepared according to the technical scheme of this embodiment 2.97 Eu 0.03 (PO 4 ) 2 Comparison of X-ray powder diffraction pattern after hydration with standard cards PDF#290359 and PDF#090432.

[0031] See attached...

Embodiment 2

[0034] Take 1 gram of alpha-Ca 2.94 Eu 0.06 (PO 4 ) 2 Powder immersed in 100ml 0.25M K 2 HPO 4 Hydrate in the buffer solution, the pH value of the buffer solution is 7.4, the temperature is 37±2°C, and the hydration time is 0~168h. Ca that will be hydrated for different times 2.94 Eu 0.06 (PO 4 ) 2 The powder was taken out from the buffer solution, washed three times with deionized water, and then dried in a constant temperature box at 70°C for 12 hours. Finally, the content of hydroxyapatite in the hydrate was analyzed by XRD, and the excitation spectrum was detected by PL. , calculate the CIE color coordinates of its spectrum or / and take photos of fluorescence excited by 365nm ultraviolet light.

Embodiment 3

[0036] Take 1 gram of alpha-Ca 2.91 Eu 0.09 (PO 4 ) 2 Powder immersed in 100ml 0.25M K 2 HPO 4 Hydrate in the buffer solution, the pH value of the buffer solution is 7.4, the temperature is 37±2°C, and the hydration time is 0~168h. Ca that will be hydrated for different times 2.91 Eu 0.09 (PO 4 ) 2 The powder was taken out from the buffer solution, washed three times with deionized water, and then dried in a constant temperature box at 70°C for 12 hours. Finally, the content of hydroxyapatite in the hydrate was analyzed by XRD, and the excitation spectrum was detected by PL. , calculate the CIE color coordinates of its spectrum or / and take photos of fluorescence excited by 365nm ultraviolet light.

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Abstract

The invention relates to a fluorescent tracing method of Alpha-tricalcium phosphate hydration process. Eu<2+> / Eu<3+> co-doped Alpha-tricalcium phosphate [Alpha-Ca<3(1-x)>Eu<3x>(PO4)2, 0.001<=x<=0.1] is gradually hydrated to form bone-like hydroxyapatite in a phosphate buffer solution, a simulation body liquid or implantation animal or an environment in a human body, the ultraviolet excitation spectrum intensity corresponding to Eu<2+> and Eu<3+> are respectively attenuated with different degrees, and CIE chromatic coordinate or fluorescent color is gradually changed to achieve in-situ real-time fluorescent tracing of the Alpha-tricalcium phosphate hydration process. The Alpha-Ca<3(1-x)>Eu<3x>(PO4)2 has the advantages of good biological compatibility, no biological toxicity, long fluorescent lifetime, high light-emitting intensity and continuous and stable light-emitting performance, and thus, the Alpha-Ca<3(1-x)>Eu<3x>(PO4)2 can be used as a biological fluorescent marker used in clinical research.

Description

technical field [0001] The invention relates to the application field of bioactive fluorescent tracer technology, in particular to a Eu 2+ / Eu 3+ A Fluorescent Tracing Method for the Hydration Process of Codoped α-Tricalcium Phosphate. Background technique [0002] Calcium phosphate cement (Calcium phosphate cement, referred to as CPC) was first successfully developed by Brown and Chow in the United States in the 1980s. Composed of various calcium phosphate salts, water, phosphate solution, dilute phosphoric acid, normal saline or plasma are often used as the curing solution. The CPC formed by mixing the solid phase powder and the solidification liquid is easy to be molded or injected, and the final solidified product is bone-like apatite which is similar to the inorganic composition and crystal phase structure of bone tissue. Because CPC can be accurately shaped and filled according to the bone defect or directly injected into the bone defect in the body with a syringe, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/64
CPCG01N21/643G01N2021/6439
Inventor 徐传艳李亚东朱阳光李亚军
Owner SUZHOU DINGAN ELECTRONICS TECH
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