Epidermal cell culture medium and methods and applications for preparing fibroblasts and epidermal cells from the same tissue

Abstract

The invention provides an epidermal cell culture medium and a method for preparing fibroblasts and epidermal cells based on a same tissue, and an application thereof, and relates to the field of cellengineering. The epidermal cell culture medium is mainly composed of a basic culture medium, bFGF and EGF, also contains platelet lysate, and can make the cultured epidermal cells have stable cell morphology and good cell activity. The method for preparing the fibroblasts and the epidermal cells based on the same tissue utilizes the difference of the migration time of the fibroblasts and the epidermal cells during tissue culture, the fibroblasts and the epidermal cells are digested respectively and then passage is performed, and different culture media are added; therefore, the fibroblasts andthe epidermal cells are prepared directly from the same tissue, the utilization rate of the tissue is improved, and the problem of the lack of a method for preparing fibroblasts and epidermal cells from the same tissue in the prior art is solved.

Description

technical field [0001] The invention relates to the field of cell engineering, in particular to a culture medium for epidermal cells and a method and application for preparing fibroblasts and epidermal cells based on the same tissue. Background technique [0002] Human skin fibroblasts and skin epidermal cells are two important cells that make up human skin. Skin fibroblasts have the function of secreting collagen, elastic fibers and other extracellular matrices, which play an important role in maintaining skin elasticity and preventing skin relaxation and wrinkles. Skin epidermal cells can secrete keratin, which is the body's first line of defense against pathogen infection. Therefore, these two types of cells have a wide range of applications in the fields of medical plastic surgery and skin damage repair. In addition, skin fibroblasts and epidermal cells, as in vitro research models, play an important role in drug toxicity experiments, drug screening and artificial skin...

AI Technical Summary

Problems solved by technology

Therefore, these two traditional cell separation methods can only separate one type of cells from one skin tissue at a time, that is, separate fibroblasts or epidermal cells from the skin tissue at a time, and cannot prepare these two types of cells at the same time, resulting in skin waste of material

[0004] Especially in the research and application of tissue engineering, the seed cells need to come from the same individual. The traditional method requires surgery to remove two pieces of skin tissue or take out a larger piece of skin tissue, and separate skin fibroblasts and skin epidermal cells respectively. On the one hand, it causes Waste of skin tissue, on the one hand, it needs to increase the size of the surgical wound, which makes the wound heal slowly, and there is a risk of leaving scars

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