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Special medium for rapid propagation of arundo donax and cultivation method of arundo donax

A technology of culture medium and basic culture medium, which is applied in horticultural methods, botany equipment and methods, applications, etc., can solve the problems of not being able to meet the needs of gardens and ecology, and that reeds cannot be propagated by seeds, and achieve the goal of improving reproductive efficiency Effect

Active Publication Date: 2019-08-16
YUNCHENG UNIVERISTY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003]Since reed bamboo cannot be propagated by seeds, the traditional tiller propagation is far from meeting the needs of gardens and ecology

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] A special culture medium for rapid propagation of Arundis, consisting of the following components: MS basic medium, 6-benzylaminoadenine (6-BA) 3.5mg / L, indolebutyric acid (IBA) 0.8mg / L, chloride Sodium 150mmol / L, adenosine triphosphate (ATP) 0.15mg / L.

[0020] A kind of rapid propagation and cultivation method of reed bamboo utilizing above-mentioned special culture medium, comprises the steps:

[0021] (1) Selection and disinfection of explants Select the axillary buds of Reed bamboo as explants, wash them under running water for 2 hours, then move them into an ultra-clean workbench, soak them in 72% alcohol for 45 seconds, and then move them into ultrasonic cleaning with sodium hypochlorite solution Shake in the machine for 6 minutes, then rinse 4 times with sterile water;

[0022] (2) Inoculation and cultivation of the aseptic system Under aseptic conditions, cut the 0.5-2 cm bud stem section, inoculate it into a culture bottle equipped with a special medium, and o...

Embodiment 2

[0029] A special culture medium for rapid propagation of Arundis, consisting of the following components: MS basic medium, 6-benzylaminoadenine (6-BA) 5mg / L, indolebutyric acid (IBA) 0.5mg / L, sodium chloride 200mmol / L, adenosine triphosphate (ATP) 0.1mg / L.

[0030] A kind of rapid propagation and cultivation method of reed bamboo utilizing above-mentioned special culture medium, comprises the steps:

[0031] (1) Selection and disinfection of explants Select the axillary buds of Reed bamboo as explants, wash them under running water for 3 hours, then move them into an ultra-clean workbench, soak them in 70% alcohol for 60 seconds, and then move them into ultrasonic cleaning with sodium hypochlorite solution Shake in the machine for 5 minutes, then rinse with sterile water 5 times;

[0032] (2) Inoculation and cultivation of the aseptic system Under aseptic conditions, cut the 0.5-2cm bud stem section, inoculate it into a culture bottle equipped with a special medium, and obtai...

Embodiment 3

[0039] A special culture medium for rapid propagation of Arundis, consisting of the following components: MS basic medium, 6-benzylaminoadenine (6-BA) 2mg / L, indolebutyric acid (IBA) 1mg / L, sodium chloride 120mmol / L, adenosine triphosphate (ATP) 0.2mg / L.

[0040] A kind of rapid propagation and cultivation method of reed bamboo utilizing above-mentioned special culture medium, comprises the steps:

[0041] (1) Selection and disinfection of explants Select the axillary buds of Reed bamboo as explants, wash them under running water for 1.5 hours, then move them into an ultra-clean workbench, soak them in 75% alcohol for 20 seconds, and move them into ultrasonic cleaning with sodium hypochlorite solution Shake in the machine for 7 minutes, then rinse twice with sterile water;

[0042] (2) Inoculation and cultivation of the aseptic system Under aseptic conditions, cut the 0.5-2cm bud stem section, inoculate it into a culture bottle equipped with a special medium, and obtain the ...

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PUM

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Abstract

The invention discloses a special medium for rapid propagation of arundo donax. The medium comprises the following components: an MS basic medium, 6-benzylaminoadenine (6-BA) of 1-8 mg / L, indolebutyric acid (IBA) of 0.2-1.5mg / L, sodium chloride of 50-300mmol / L, and adenine nucleoside triphosphate (ATP) of 0.05-0.5mg / L. According to the special medium, based on a traditional MS basic medium and cytokinin, sodium chloride and ATP are added, and especially that ATP is added in batches, so that the reproductive efficiency of arundo donax can be significantly improved.

Description

technical field [0001] The invention relates to the technical field of reed bamboo cultivation, in particular to a special culture medium for rapid propagation of reed bamboo and a cultivation method thereof. Background technique [0002] At present, Arundo donax is a perennial tall herbaceous plant of the genus Arundo donax in the Poaceae family, with well-developed rhizomes. It has strong adaptability and has a certain tolerance to saline-alkali, drought, and barrenness. It is known as the "pioneer plant" in low-lying saline-alkali land. Arundis not only has the ecological benefits of preventing soil degradation and increasing soil carbon content and organic matter, but also has great potential for remediating heavy metal pollution in wetlands. In recent years, the utilization value of Arundis as a clean bioenergy has also gradually attracted people's attention, and perennial herbaceous lignocellulosic plants are considered to be the most suitable for biomass bioenergy pr...

Claims

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Application Information

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IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008Y02P60/40Y02A40/22
Inventor 刘缙王亚红王娟付蓉徐夏梦李肖凡魏熙呈刘璐
Owner YUNCHENG UNIVERISTY
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