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Novel fermentation tank and application thereof for fermented preparation of ethyl alcohol from immobilized yeast

A technology for immobilizing yeast and fermenters, which is applied to methods such as methods of immobilization on or in inorganic carriers, methods for immobilization on/in organic carriers, and methods for supporting/immobilizing microorganisms, can solve problems such as poor results, and achieve Improve the heat dissipation effect, reduce the material blockage, and the effect of low investment cost

Pending Publication Date: 2019-08-13
NANJING HIGH TECH UNIV BIOLOGICAL TECH RES INST CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] The technical problem to be solved by the present invention is to provide a surface-immobilized yeast fermentation method and equipment to solve the problems of poor effect in the prior art

Method used

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  • Novel fermentation tank and application thereof for fermented preparation of ethyl alcohol from immobilized yeast

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Example 1 Preparation of fuel ethanol by using molasses as fermentation raw material and adopting bamboo fiber immobilized yeast

[0039] First, put bamboo fiber (thickness 0.15cm) cut into diameter 15cm into polyethylene (density 0.94g / cm 3 ) made as figure 1 In the spherical grid with a diameter of 20cm shown, the immobilization carrier was prepared; then the immobilization medium was 2 The filling amount per L is placed in a 20-ton reactor with a height-to-diameter ratio of 3:1. Then the cultured bacterial classification is added in the fermenter that 4 layers of grating plates are housed (every layer of feed pipe in the tank (i.e. figure 1 The shown flush water flow tube) nozzle is located in the middle position, and the multi-directional nozzle is facing around), and the flow rate is 10m 3 The / h cycle is fixed at 36h. Until the bacterial cell adsorption concentration is 200 million / ml. Then drain to waste. Then add the molasses fermentation medium (total sug...

Embodiment 2

[0040] Example 2 Production of fuel ethanol using cassava liquefied mash medium as fermentation raw material and cotton fiber immobilized yeast

[0041] First, put the cotton fiber cut into a diameter of 18 cm into a spherical grid with a diameter of 25 cm made of polyethylene (density 0.92 g / cm 3 ), prepared as an immobilization medium; then the immobilization medium was 120cm 2 The filling amount per L is placed in a 20-ton reactor with a height-to-diameter ratio of 3:1. Then the cultured strains are added to the fermenter equipped with 3 layers of grid plates (the nozzle of each layer of feed pipe in the tank is located in the middle, and the multi-directional nozzle is facing all around), with a flow rate of 10m 3 The / h cycle is fixed at 30h. Until the bacterial cell adsorption concentration is 250 million / ml. Then prepare cassava liquefied mash: the ratio of solid to liquid is 1:2.5, add liquefaction enzyme (15U / g cassava dry weight) to liquefy at 85°C for 2 hours, an...

Embodiment 3

[0042] Example 3 Production of fuel ethanol using high-concentration cassava liquefied mash medium as fermentation raw material and immobilized yeast with cotton fiber

[0043] First, put the cotton fiber cut into a diameter of 8 cm into a spherical grid with a diameter of 12 cm made of polyvinyl fluoride (density 0.89 g / cm 3 ), prepared as an immobilization medium; then the immobilization medium was 110cm 2 The filling amount per L is placed in a 20-ton reactor with a height-to-diameter ratio of 3:1. Then add the cultured bacteria into the fermenter equipped with 4 layers of grid plates (the feed pipes in the tank are arranged in a circle against the tank wall, and the circular pipes are drilled downwards to install a one-way nozzle. It is at an angle of 40° to the tangent direction of the circular pipeline, and the nozzle is at an angle of 35° to the horizontal plane), so that the outflowing liquid is in a spiral state downwards), with a flow rate of 15m 3 The / h cycle is ...

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Abstract

The invention discloses a surface immobilized yeast fermentation method and equipment. A fiber material is fixed to a spherical mesh, a fermentation tank is filled with spheres in a layered mode, thefermentation tank is provided with flow dividing pipelines in a layered mode, and the circulation mode is adopted for yeast immobilization and continuous intermittent fermentation. The technology overcomes the defect in the existing immobilization technology, the immobilization method is low in input cost, convenient to implement, stable in technology and good in mass transfer and adsorption effect, no strict limit is caused to raw materials, the method can be used for starch raw material residue fermentation, and the method is easily applied industrially.

Description

technical field [0001] The invention belongs to the technical field of industrial biology, and in particular relates to a surface-immobilized yeast fermentation method and equipment. Background technique [0002] With the shortage of natural resources and the increasingly prominent environmental problems, the status of biological fermentation technology is becoming more and more important, and it has also achieved rapid development in recent years. Due to its many advantages, immobilization technology has become an important research hotspot in biological fermentation. Although the research on immobilization technology has a long history, very few of them are actually applied to industrial production. The reason is that the immobilization medium has low mechanical strength, poor mass transfer effect, and difficult operation. Previously, we applied for the surface immobilized yeast fermentation technology (such as patent number ZL201210538977.X), that is, to attach speciall...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/08C12N11/14C12P7/06C12P7/08C12M1/00
CPCC12M25/06C12N11/08C12N11/14C12P7/06C12P7/08Y02E50/10
Inventor 陈勇刘庆国应汉杰佟毅赵南邹亚男甘诚
Owner NANJING HIGH TECH UNIV BIOLOGICAL TECH RES INST CO LTD
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