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Bacterial RNase E truncation body and application thereof

A technology of bacteria and short body, applied in RNaseE truncation body and its application field, to achieve the effect of inhibiting growth and proliferation

Active Publication Date: 2019-08-13
GUIZHOU MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although biological therapy such as tumor immunotherapy is also used in the clinical treatment of tumors, the effect still needs to be further improved

Method used

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  • Bacterial RNase E truncation body and application thereof
  • Bacterial RNase E truncation body and application thereof
  • Bacterial RNase E truncation body and application thereof

Examples

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Embodiment 1

[0028] Example 1: A bacterial RNase E truncated body, the protein sequence of the bacterial RNase E truncated body is shown in SEQ ID NO.1.

[0029] A gene encoding a bacterial RNase E truncated body, the coding gene sequence of the bacterial RNase E truncated body is shown in SEQ ID NO.2.

[0030] A gene encoding a bacterial RNase E truncated body, a vector containing the encoded gene of a bacterial RNase E truncated body: the linearized backbone vector pLVX-AcGFP-N1 and the target gene are digested with Xho I and EcoR I, and connected, The lentiviral gene expression vector pLVX-REt-AcGFP was obtained through transformation, screening, expanded cultivation and identification.

[0031] The application of a bacterial RNase E truncation body, the application of the bacterial RNase E truncation body in inhibiting tumors.

[0032] A kind of preparation process step of bacterium RNase E truncated body is as follows:

[0033] 1. Cloning and transformation of RNase E gene

[0034]...

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Abstract

The invention discloses a bacterial RNase E truncation body and application thereof. A protein sequence of the bacterial RNase E truncation body is shown in SEQ ID NO.1. An N end of the RNase E is used for degrading a functional domain of RNA, and a nucleotide coding sequence of the truncation body (Ret) is synthesized in vitro, an SV40 nuclear localization sequence is added at a 5' end of the RNase E, so that the RNase E is expressed in a cancer cell nucleus, various RNAs including mRNA are directly degraded in the cell nucleus, and the biosynthesis of oncoprotein is subjected to interference, thereby inhibiting the growth, proliferation, migration and invasion of cancer cells, and being expected to achieve the effect of inhibiting tumors.

Description

technical field [0001] The invention relates to a bacterial RNase E truncated body and an application thereof, and belongs to the technical field of the RNase E truncated body and its application. Background technique [0002] According to the "2018 Global Cancer Statistics" report, there were about 18.1 million new cancer cases and 9.6 million cancer deaths in the world in 2018. Among the 18.1 million new cases, China ranked first in the world in terms of cancer incidence and mortality. According to statistical analysis, in 2018, my country had about 3.804 million new cases and 2.296 million deaths, which means that for every 100 new cancer patients in the world, China accounted for 21 people. In my country, the top five cancers with the highest incidence are lung cancer, breast cancer, gastric cancer, colorectal cancer, and liver cancer. At present, although cancer treatment methods and methods are diversified, including surgical resection, chemotherapy, radiotherapy and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N9/22C12N15/55C12N15/867A61K48/00A61K38/46A61P35/00
CPCC12N9/22C12N15/86A61K48/005A61P35/00C12N2740/15043C12N2800/107A61K38/00
Inventor 孙达权石静余畅陈相屹石松徐莹莹胡桐张钦真
Owner GUIZHOU MEDICAL UNIV
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