Consumable for extracting bioactive substances, and automation device and method

Abstract

The invention provides a consumable for extracting bioactive substances, and an automation device and method. The consumable comprises a sample reaction component and a plurality of tubular componentshermetically preloaded with reagents, the sample reaction component comprises a sealed chamber in which various reactions occur, a first hole for injecting a sample, a plurality of second holes for receiving the tubular components, a third hole for discharging waste liquid and a fourth hole for collecting the bioactive substances, the first hole, the second holes, the third hole and the fourth hole all communicate with the sealed chamber and are all sealed, one end of each of the plurality of tubular components is provided with a piston that is hermetically jointed with an inner wall of the tubular component and can slide therein, the other end is provided with a reagent outlet, and at least one of the preloaded reagents in the plurality of tubular components contain magnetic particles therein. When the consumable and the device provided by the invention are used for extracting the bioactive substances, the even mixing of the liquid is achieved by rotating and oscillating the sealed chamber containing the liquid, thereby avoiding the splashing of the liquid and the formation of aerosol, and eliminating the possibility of cross contamination.

Description

technical field [0001] The invention relates to the field of life sciences, in particular to a consumable, an automatic device and an automatic method for extracting biologically active substances. Background technique [0002] Modern clinical disease diagnosis, forensic examination, environmental microbial detection, food safety detection, molecular biology and other fields need to extract biologically active substances, such as cells, proteins, nucleic acids (which can be divided into ribonucleic acid (RNA for short) and deoxyribonucleic acid (referred to as DNA)) and other biologically active substances. The extraction of biologically active substances especially involves separation and purification steps. However, due to the complexity of biological samples, separation and purification are not easy, especially to purify multiple samples is quite difficult, not only need to choose the appropriate purification technology, but also work The amount is also very large, and i...

AI Technical Summary

Problems solved by technology

This method overcomes the defects of the above-mentioned suction method, but the magnetic device oscillates at high frequency during the up-and-down movement process, which is prone to liquid splashing and aerosol. This aerosol is a relatively stable liquid or solid particle uniformly dispersed in the gas. The suspension system carries the sample molecules. When the aerosol or liquid droplets dispersed inside the instrument enter other samples, it will cause cross-contamination of the samples.

Due to technical and structural constraints, the nucleic acid extractor using the magnetic rod method cannot achieve a completely closed sample extraction environment. It can only be disinfected by ultraviolet light or equipped with a high-power fan to extract the gas from the machine to reduce the possibility of cross-contamination. , but cannot fundamentally avoid cross-contamination between samples

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