Method for purifying ligneous alpha-linolenic acid for medicine and application of such method

A purification method and technology of linolenic acid, applied in the separation/purification of carboxylic acid compounds, organic chemical methods, chemical instruments and methods, etc., can solve the problems of low purity of α-linolenic acid, achieve high separation and purification effect, and improve purity Effect

Inactive Publication Date: 2019-07-26
HENAN AGRICULTURAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] On the one hand, the present invention improves the existing urea inclusion method, can extract high-purity α-linolenic acid from peony seed oil, and overcomes the defect that the purity of α-linolenic acid obtained by the existing urea inclusion method is not high

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The purification method of α-linolenic acid is as follows:

[0022] (1) Saponification: Prepare 500 mL of 0.5 mol / L sodium hydroxide ethanol solution, add it to 100 g of edible peony seed oil, stir and saponify at 80°C for 1 hour, and obtain a saponified solution;

[0023] (2) Acid hydrolysis: take the saponified solution, adjust the pH to 2-3 with 15wt% hydrochloric acid;

[0024] (3) Extract the saponified liquid after acidolysis with sherwood oil 5 times, then mix each time extracting solution, adjust pH to neutrality, get sherwood oil phase, reclaim sherwood oil wherein (this recovering method can be decompression Known methods such as distillation) to obtain mixed fatty acids;

[0025] (4) Mix the fatty acid, urea, and ethanol according to the weight ratio of mixed fatty acid: urea: ethanol = 1:3:8, mix evenly, freeze at -50°C for 10h, filter, and after the ethanol is recovered from the filtrate, adjust the pH to 2-3, and use Petroleum ether extraction, recovery ...

Embodiment 2

[0027] The purification method of α-linolenic acid is as follows:

[0028] (1) Saponification: Prepare 200 mL of 2 mol / L sodium hydroxide ethanol solution, add it to 100 g of edible peony seed oil, stir and saponify at 50°C for 1 hour, and obtain a saponified solution;

[0029] (2) Acid hydrolysis: take the saponified solution, adjust the pH to 2-3 with 15wt% hydrochloric acid;

[0030] (3) Extracting the saponified liquid after acidolysis with petroleum ether for 5 times, then mixing each extract, adjusting the pH to neutral, taking the petroleum ether phase, reclaiming the petroleum ether therein, to obtain mixed fatty acids;

[0031] (4) Mix the fatty acid, urea, and ethanol according to the weight ratio of mixed fatty acid: urea: ethanol = 1:3:8 and mix evenly, freeze at -30°C for 20h, filter, and after the ethanol is recovered from the filtrate, adjust the pH to 2-3, and use Petroleum ether extraction, recovery of petroleum ether in the extract, to obtain purified α-lino...

Embodiment 3

[0033] The purification method of α-linolenic acid is as follows:

[0034] (1) Saponification: Prepare 50 mL of 5 mol / L sodium hydroxide ethanol solution, add it to 100 g of edible peony seed oil, stir and saponify at 45°C for 1 hour, and obtain a saponified solution;

[0035] (2) Acid hydrolysis: take the saponified solution, adjust the pH to 2-3 with 15wt% hydrochloric acid;

[0036] (3) Extracting the saponified liquid after acidolysis with petroleum ether for 5 times, then mixing each extract, adjusting the pH to neutral, taking the petroleum ether phase, reclaiming the petroleum ether therein, to obtain mixed fatty acids;

[0037] (4) Mix the fatty acid, urea, and ethanol according to the weight ratio of mixed fatty acid: urea: ethanol = 1:3:8 and mix evenly, freeze at -30°C for 20h, filter, and after the ethanol is recovered from the filtrate, adjust the pH to 2-3, and use Petroleum ether extraction, recovery of petroleum ether in the extract, to obtain purified α-linol...

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PUM

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Abstract

The invention discloses a method for purifying ligneous alpha-linolenic acid for medicine and application of such method. The method includes the steps of preparing a 0.2-5 mol / L sodium hydroxide-ethanol solution, adding the solution into edible peony seed oil, stirring and saponifying at 30-90 DEG C to obtain saponified liquid, and regulating the pH value to 2-3 by acid liquor; extracting the saponified liquid after acidolysis for multiple times by petroleum ether, mixing the extracting solution, regulating the pH value to be neutral and taking a petroleum ether phase so as to obtain mixed fatty acid; evenly mixing the mixed fatty acid, urea and ethanol according to the weight ratio of 1: 3: 8, freezing at 60 DEG C for 10-50 hours, filtering, recovering the ethanol from the filtrate, regulating the pH value to 2-3, extracting with the petroleum ether, and recovering the petroleum ether in the extract so as to obtain the purified alpha-linolenic acid. The method for purifying the ligneous alpha-linolenic acid for the medicine can be used for preparing the about 90% by mass alpha-linolenic acid as a medical raw material, thereby being high in application value.

Description

technical field [0001] The invention relates to a method for purifying alpha-linolenic acid, in particular to a method for extracting alpha-linolenic acid from peony seed oil by using a urea inclusion method, and the extracted alpha-linolenic acid can be used in the field of medicine. Background technique [0002] α-linolenic acid has the functions of lowering blood fat, lowering blood sugar, lowering blood pressure, enhancing immunity, anti-oxidation, delaying aging, and anti-cardiovascular and cerebrovascular diseases. It has good medicinal value and can also be used as a functional cosmetic additive. Antioxidant and anti-aging effects. [0003] Peony seed oil is rich in unsaturated fatty acids (about 85%) and various nutritional and physiologically active substances. It is praised by nutritionists as "the best oil in the world" and has high edible and medicinal value. Among them, α-linolenic acid is particularly rich in content, and α-linolenic acid is an unsaturated fat...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07C51/50C07C51/02C07C57/12
CPCC07C51/50C07C51/02C07C51/412C07B2200/09C07C57/12
Inventor 彭万喜杨亚峰张党权
Owner HENAN AGRICULTURAL UNIVERSITY
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