Micro-fluidic chip for chemiluminescence detection, and using method and reagent cleaning method thereof

Abstract

The invention provides a micro-fluidic chip for chemiluminescence detection, and a using method and a reagent cleaning method thereof, and belongs to the field of biochemical testing devices. The micro-fluidic chip comprises a substrate and a cover plate which form a sealing environment and are used for a micro-fluid to flow, the substrate is covered with the cover plate, and the substrate comprises: a sample adding hole and a diluted solution adding hole at the first layer; a sample quantification groove, a sample overflow groove, a diluted solution quantification groove and a dilution overflow groove at the second layer; an incubation groove at the third layer, solution storage grooves at the fourth layer, a detection groove at the fifth layer and a waste liquid groove at the sixth layer, wherein the sample quantification groove is communicated with the incubation groove, the diluted solution quantification groove is communicated with the incubation groove, the incubation groove is communicated with the detection groove, and the detection groove is communicated with the waste liquid groove through a hydrophobic flow channel. The separation, mixing and detection of a liquid is realized without repeatedly adding a sample, and the whole process is automatically completed, so the efficiency is increased, and the cost is realized; and the cleaning of a reaction reagent is realized, so the working efficiency is increased.

Description

technical field [0001] The invention belongs to the field of biochemical testing equipment, and relates to a microfluidic chip for chemiluminescence detection, a use method, and a reagent cleaning method. Background technique [0002] Microfluidic chip technology is a new technology for precisely manipulating and controlling nanoliter and picoliter fluids (biological sample fluids) in micron-scale flow channels. The application of this technology can make sample preparation, The basic operation units such as reaction, separation, detection, cell culture, sorting, and lysis are integrated or basically integrated on a chip of several square centimeters (or even smaller), and a network of microfluidic channels is formed to control the flow of fluid throughout the system. A technical platform used to replace various functions of conventional chemical or biological laboratories. The basic feature and biggest advantage of microfluidic lab-on-a-chip is the flexible combination and...

AI Technical Summary

Problems solved by technology

[0005] At present, in the process of rotating microfluidic chips on the market, most of them are pneumatically or mechanically driven, which is not convenient for the integration and miniaturization of equipment. Moreover, microfluidic chips require a four-layer structure for sealing, increasing It reduces the cost and processing difficulty of the chip manufacturing process, which is not conducive to automated batch industrial production, and can only realize the chemiluminescence detection of trace liquids for some immunoassay reagent items, but there is no related equipment for simultaneous detection of multiple items

Images