Fermentation enzyme production method for bacillus producing sarcosine oxidase

Abstract

The invention relates to a fermentation enzyme production method for bacillus producing sarcosine oxidase which belongs to the field of bioengineering. Fermentation enzyme production is performed by utilizing an LYH18 strain with a preservation number of CGMCC No.17306 as a bacterial strain, thereby preparing the sarcosine oxidase. The fermentation medium and enzyme production method obtained by screening and optimization has the advantages of short fermentation cycle, mild condition, easy control, provided optimal fermentation condition, high enzyme production efficiency, high enzyme activity, stable enzyme activity, and the like, and is beneficial to carry-out of application research in the next step.

Description

technical field [0001] The invention relates to a method for fermenting and producing sarcosine oxidase-producing bacillus, belonging to the field of bioengineering. Background technique [0002] As an important diagnostic enzyme preparation, sarcosine oxidase is widely used in the detection of creatinine level in human serum to judge the health of renal function. Although many scholars at home and abroad have studied it, in practice There are still problems of low yield and poor thermal stability in production, and there are few domestic researches. Therefore, how to quickly screen out sarcosine oxidase with high enzyme activity and high yield suitable for industrial production is of great significance. The industrial production of oxidase is mainly obtained from microbial fermentation. [0003] Liquid fermentation technology is widely used in traditional industrial production due to its controllable fermentation conditions. However, during the fermentation process, many f...

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Problems solved by technology

[0002] As an important diagnostic enzyme preparation, sarcosine oxidase is widely used in the detection of creatinine level in human serum to judge the health of renal function. Although many scholars at home and abroad have studied it, in practice There are still problems of low yield and poor thermal stability in production, and there are few domestic researches. Therefore, how to quickly screen out sarcosine oxidase with high enzyme activity and high yield suitable for industrial production is of great significance. The industrial production of oxidase is mainly obtained by microbial fermentation

[0003] Liquid fermentation technology is widely used in traditional industrial production due to its controllable fermentation conditions. However, during the fermentation process, many factors will affect the growth of microorganisms and the production of enzymes. The most important factor is the composition of the medium. Secondly, all other physical and chemical factors such as fermentation temperature, pH, metal ions, etc. will also affect the fermentation, and then affect the final enzyme production of the strain

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