Liquid fertilizer for promoting growth of fruit corn rhizomes and preparation method of liquid fertilizer
A technology of liquid fertilizer and fruit corn is applied in the field of liquid fertilizer to achieve the effects of promoting root growth, enhancing plant resistance and increasing weight
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Embodiment 1
[0046] The isolation and identification of embodiment 1 Streptomyces bacterial strain (Streptomyces)
[0047] 1. Experimental materials and conditions
[0048] (1) Soil samples collected in a certain place in Chenzhou City, Hunan Province.
[0049] (2) The components per liter of the liquid medium are as follows: 3.0 g of beef extract, 10.0 g of peptone, 5.0 g of sodium chloride, dilute to 1 liter of water, and the pH is 7.5.
[0050] The components per liter of solid medium are as follows: beef extract 3.0g, peptone 10.0g, sodium chloride 5.0g, agar 15-25g, water to 1 liter, pH 7.5.
[0051] The culture medium was divided into aliquots and sterilized in an autoclave (121°C, 30min).
[0052] (3) Sterile operating conditions: All utensils and utensils must be sterilized in an autoclave (121°C, 30min), and operations such as inoculation are carried out in an ultra-clean workbench.
[0053] (4) Conditions for culturing strains: place them in a light incubator (14L: 10D) at 25±...
Embodiment 2
[0068] Embodiment 2 A kind of preparation and application of the liquid fertilizer that promotes the growth of fruit corn rhizome
[0069] A preparation method for promoting the growth of fruit corn rhizomes comprises the following steps:
[0070] S1. Preparation of culture medium: soak 1000g of soybeans for 10 hours, put them into a refiner and grind them into soybean milk, filter out the bean dregs, put the filtrate in a clean fermenter, add white sugar to the filtrate, add water to The concentration of sugar in the medium is 100g / L, and the pH is 7.5;
[0071] S2. inoculation: get the liquid spawn of Streptomyces bacterial strain " TY-001# ", inoculate in the culture medium, stir evenly; There are 200,000,000 viable bacteria per milliliter in the liquid spawn; The ratio of liquid spawn and culture medium 165g:250L;
[0072] S3. Fermentation: Turn on the oxygen pump, continuously feed oxygen into the fermenter during the fermentation process, and add an appropriate amount ...
Embodiment 3
[0075] Embodiment 3 A kind of preparation and application of the liquid fertilizer that promotes fruit corn rhizome growth
[0076] A preparation method for promoting the growth of fruit corn rhizomes comprises the following steps:
[0077] S1. Preparation of culture medium: soak 1000g of soybeans for 10 hours, put them into a refiner and grind them into soybean milk, filter out the bean dregs, put the filtrate in a clean fermenter, add white sugar to the filtrate, add water to The concentration of sugar in the medium is 100g / L, and the pH is 7.5;
[0078] S2. inoculation: get the liquid spawn of Streptomyces bacterial strain " TY-001# ", inoculate in the culture medium, stir evenly; There are 200,000,000 viable bacteria per milliliter in the liquid spawn; The ratio of liquid spawn and culture medium 165g:250L;
[0079] S3. Fermentation: Turn on the oxygen pump, continuously feed oxygen into the fermenter during the fermentation process, and add an appropriate amount of defo...
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