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Method and system for determining dissociation rate constant based on affinity chromatography

A dissociation rate constant and measurement system technology, applied in the field of methods and measurement systems, can solve problems such as weak interactions and achieve low-cost effects

Inactive Publication Date: 2019-06-28
李雪明
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0008] U.S. Patent US20080293587 discloses a biological target screening method based on affinity chromatography with transient weak interactions between ligands and receptors, mainly focusing on high-throughput screening of ligand-receptor affinity, that is, based on intermolecular The binding equilibrium constant Ka of the interaction was screened, but the rate constant was not involved

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Embodiment

[0028] A method and a measurement system for determining a dissociation rate constant based on affinity chromatography, the method and the measurement system are as follows:

[0029] S1. Take an appropriate amount of meloxicam reference substance, add methanol to make a 0.25 mg / mL meloxicam stock solution, take an appropriate amount of meloxicam stock solution, and dilute it into 2.5 μg / mL meloxicam with mobile phase The test solution, ready for use;

[0030] S2. Take an appropriate amount of paracetamol reference substance, add methanol to make a 1.2 mg / mL paracetamol stock solution, take an appropriate amount of paracetamol stock solution, and dilute it into 0.45 μg / mL paracetamol stock solution with mobile phase The test solution, ready for use;

[0031] S3. Take an appropriate amount of uracil reference substance, add water to make a 1.2 mg / mL uracil stock solution, take an appropriate amount of uracil stock solution, and dilute it with mobile phase to form a uracil test ...

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Abstract

The invention discloses a method and system for determining dissociation rate constant based on affinity chromatography, and belongs to the technical field of affinity chromatography. The method comprises the following steps: S1, taking a proper amount of meloxicam reference substances, adding methanol to prepare 0.25 mg / mL of a meloxicam stock solution, taking a proper amount of the meloxicam stock solution, and diluting with a mobile phase to obtain 2.5 mu g / mL of a meloxicam test substance solution for later use; S2, taking a proper amount of an acetaminophen reference substances, adding methanol to prepare 1.2 mg / mL of an acetaminophen stock solution, taking a proper amount of the acetaminophen stock solution, and diluting with a mobile phase to obtain 0.45 mu g / mL of an acetaminophentest substance solution for later use; and S3, taking a proper amount of uracil reference substances. The kinetic rate constant of supramolecular weak interaction can be measured by using a conventional chromatographic analysis method, and the method and system for determining the dissociation rate constant based on affinity chromatography have unique advantages in the aspects of detection of drugs with low concentration and poor water solubility and low-cost and rapid determination of supramolecular interaction kinetic parameters.

Description

technical field [0001] The invention relates to the technical field of affinity chromatography, and more specifically relates to a method and a measurement system for determining dissociation rate constants based on affinity chromatography. Background technique [0002] Molecules are generally aggregates of two or more molecules. The supramolecules formed by drugs and excipients are usually formed by non-covalent interactions between host molecules (supramolecular carriers) and guest molecules (drugs) through hydrogen bonds and van der Waals forces. Molecular complexes, drugs and supramolecular carriers, such as cyclodextrins, crown ethers, and dendrimers, can improve the water solubility, bioavailability and targeting of drugs after forming supramolecular complexes. Inflammation and other fields play an important role. [0003] The interaction between host and guest molecules, that is, host-guest recognition, is the basis for supramolecules to exert their special functions...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 李雪明黄年霞张建明
Owner 李雪明
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