In-situ observation method of root hair of plants

Abstract

The invention provides an in-situ observation method of root hair of plants. The method comprises the steps of utilizing a double-stretching polypropylene film for covering a culture medium block in which seeds are dibble-seeded, and manufacturing sheets. The in-situ observation method of the root hair of the plants is simple and reliable, deformation or damage of root hair cells of the plants andinterference of intracellular autofluorescence signals caused by the damage of the root hair cells can be avoided, and in-situ microscopic observation and imaging of the root hair cells are achieved;meanwhile, seed germination, root hair growth and fluorescence signal intensity are not influenced, and the method achieves a positive promoting effect on cytological study of the root hair.

Description

technical field [0001] The invention relates to the technical field of plant microscopy, in particular to an in-situ observation method of plant root hairs. Background technique [0002] Root hairs are important organs of terrestrial plants, responsible for absorbing water and inorganic salts, and participating in the fixation of underground parts of plants in soil. The study of the structure and function of root hairs is a research hotspot in plant cell biology, plant physiology and developmental biology. [0003] At present, when performing microscopic observation or fluorescence observation and analysis experiments on root hairs, it is basically necessary to remove the root hairs from the solid culture medium, make slides, and cover and flatten them with a cover glass. During this process, it is very easy to cause damage to the root hairs when they are taken out of the solid medium. At the same time, using a cover glass to make a slide and then observe it will change the...

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Problems solved by technology

During this process, it is very easy to cause damage to the root hairs when they are taken out of the solid medium. At the same time, using a cover glass to make a slide and then observe it will change the extension shape of the root hairs, causing the root hairs to be twisted, Bending and other abnormalities will affect the observation of root hair microscopic morphology; second, it will lead to hypoxia and dehydration of root hair cells, and accelerate cell death, which is not suitable for fluorescence observation and analysis experiments that require long-term observation of living cells

[0004] At the same time, in the fluorescence observation of root hair cells, because the cell wall of root hair cells is very thin, the cell shape is easy to change after being affected by external factors (such as external force, pH value, light intensity, etc.), these stress responses will make root hair cells Internal autofluorescence, which seriously interferes with cell phenotype observation and intracellular signal research

[0005] Therefore, in the microscopic observation of plant root hair cells, how to avoid root hair distortion, bending and other abnormalities caused by changing the extension shape of root hair cells, and then avoid the interference of intracellular fluorescent signals caused by the shape damage of root hair cells, so that plant root hairs can be inspected. Long-term fluorescence observation and analysis has become a new problem for plant microscopy techniques that have not been studied or reported at present, and the existing techniques cannot realize in situ microscopic observation and imaging of root hair cells, nor can they provide a method that can Under the condition of not changing the normal growth state of root hair, the method of long-term in situ microscopic observation of root hair cells in bright field and fluorescence

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