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Muscle protein purifying method

A purification method and technology of muscle protein, applied in the purification of three kinds of muscle protein, the field of muscle protein purification, can solve the problems of increased economic cost, complicated separation process, large degree of protein denaturation, etc., to save purification cost, simple purification process, The effect of a small degree of protein denaturation

Inactive Publication Date: 2019-06-28
SHANGHAI OCEAN UNIV
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AI Technical Summary

Problems solved by technology

The degree of protein denaturation is greater under high ionic strength, and the separation process is complicated
In addition, the use of high ionic strength to dissociate myofibrillar protein requires high equipment, which increases economic costs and is not conducive to popularization.

Method used

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Embodiment Construction

[0030] To achieve the above object, the present invention provides the following technical solutions:

[0031] The influence of different solution matrices on separation and purification: adjust the concentration of KCl, ATP, and ammonium sulfate (A.S.), use ATPase activity as the indicator parameter of protein denaturation, analyze the dissociation and precipitation rules of each myofibrillar protein component, and determine the myofibrillar protein components. Separation and purification conditions of actin, myosin and paramyosin.

[0032] A muscle protein purification method, the clam described in the present invention cuts off the foot muscle, collects the foot muscle tissue, puts it in a bag and places it on ice as a sample muscle.

[0033] The foot muscle tissue blocks were fixed in Carnot's solution, embedded in conventional paraffin, sectioned, stained with hematoxylin-eosin, and then observed histologically using an optical microscope.

[0034] The muscle of the samp...

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Abstract

A muscle protein purifying method adjusts the concentrations of KCl, ATP and ammonium sulfate (A.S.) through the influences of different solution mediums on separation and purification and analyzes the dissociation and precipitation law of all fibrillin components and determines the separation and purification conditions of actin, myoballs and paramyosin with the ATPase activity as the protein denaturation indicating parameter. The method has the advantages that compared with a traditional protein purifying method, the novel method is low in purified protein denaturation degree and simple in purifying process, the actin, myoballs and paramyosin in smooth muscles of aquatic invertebrates are efficiently obtained through purification, and the purifying cost is saved to the maximum extent.

Description

technical field [0001] The invention relates to a protein separation and purification method, in particular to a muscle protein purification method, in particular to a purification method for obtaining three muscle proteins of aquatic invertebrates through gradient dissolution. technical background [0002] The thick filaments of muscles in aquatic invertebrates are mainly composed of paramyosin forming the core and myosin distributed on the surface, with more paramyosin in smooth muscle and more myosin in striated muscle [1] . They not only participate in physiological metabolism, but also the changes of their own characteristics directly affect the food quality. [0003] At present, the research on myosin and paramyosin in aquatic invertebrates at home and abroad mainly focuses on the contraction mechanism, thermal stability, gelation and other aspects. Most of the isolation and purification methods are to dissociate myofibrillar protein under high ionic strength (0.4-0....

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K14/435C07K1/34
Inventor 王锡昌张龙樊震宇党连魁
Owner SHANGHAI OCEAN UNIV
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