Microbial organic fertilizer and preparation method thereof
A microbial organic fertilizer and Bacillus subtilis technology, applied in organic fertilizers, fertilization devices, fertilizer mixtures, etc., can solve the problems of low organic matter content and high heavy metal content, and achieve the effect of protecting the environment
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Embodiment 1
[0027] The microbial organic fertilizer includes 4.5 parts of Bacillus thermophile, 1 part of Bacillus licheniformis, 2 parts of Bacillus subtilis, 0.5 part of Aspergillus oryzae, 0.5 part of thermophilic actinomycetes, 1 part of thermophilic fungus, and 0.5 part of Saccharomyces cerevisiae
[0028] The trace elements include 4 parts of nitrogen-containing substances, 4 parts of phosphorus-containing substances, and 2 parts of potassium-containing substances.
[0029] The present invention simultaneously provides a kind of preparation method of microbial organic fertilizer, and concrete steps are as follows:
[0030] 1) Preparation of fermentation broth After culturing thermophilic Bacillus, Bacillus licheniformis, Bacillus subtilis, Aspergillus oryzae, thermophilic actinomycetes, and thermophilic fungi in LB liquid medium, S. Carry out mixed fermentation, the fermentation time is 10 hours, and the fermentation temperature is 50°C
[0031] LB liquid medium (G / L): yeast powder...
Embodiment 2
[0037] The microbial organic fertilizer includes 4.5 parts of Bacillus thermophile, 1 part of Bacillus licheniformis, 2 parts of Bacillus subtilis, 0.5 part of Aspergillus oryzae, 0.5 part of thermophilic actinomycetes, 1 part of thermophilic fungus, and 0.5 part of Saccharomyces cerevisiae
[0038] The trace elements include 4.5 parts of nitrogen-containing substances, 4 parts of phosphorus-containing substances, and 1.5 parts of potassium-containing substances.
[0039] Provide a kind of preparation method of microbial organic fertilizer simultaneously, concrete steps are as follows:
[0040] 1) Preparation of fermentation broth After culturing thermophilic Bacillus, Bacillus licheniformis, Bacillus subtilis, Aspergillus oryzae, thermophilic actinomycetes, and thermophilic fungi in LB liquid medium, S. Carry out mixed fermentation, fermentation time is 11 hours, fermentation temperature is 55 ℃.
[0041] LB liquid medium (G / L): yeast powder 10G, peptone 10G, sodium chloride...
Embodiment 3
[0047] The microbial organic fertilizer includes 4.5 parts of Bacillus thermophile, 1 part of Bacillus licheniformis, 2 parts of Bacillus subtilis, 0.5 part of Aspergillus oryzae, 0.5 part of thermophilic actinomycetes, 1 part of thermophilic fungus, and 0.5 part of Saccharomyces cerevisiae
[0048] The trace elements include 4 parts of nitrogen-containing substances, 4.5 parts of phosphorus-containing substances, and 1.5 parts of potassium-containing substances.
[0049] Provide a kind of preparation method of microbial organic fertilizer simultaneously, concrete steps are as follows:
[0050] 1) Preparation of fermentation broth After culturing thermophilic Bacillus, Bacillus licheniformis, Bacillus subtilis, Aspergillus oryzae, thermophilic actinomycetes, and thermophilic fungi in LB liquid medium, S. Carry out mixed fermentation, fermentation time is 12 hours, fermentation temperature is 53 ℃.
[0051] LB liquid medium (G / L): yeast powder 10G, peptone 10G, sodium chloride...
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