Laser light source speckle measurement method, speckle suppression device and its parameter optimization method

Abstract

The invention discloses a laser light source speckle measurement method, a speckle suppression device and a parameter optimization method thereof. The laser light source speckle measurement method comprises the following steps: laser light emitted by a laser generator is irradiated onto a fluorescence imaging camera; the fluorescence imaging camera Receive laser light within the preset exposure time and generate corresponding image information; collect image information and form a Gaussian distribution image containing speckle, where the speckle appears as high-frequency noise; calculate the speckle after processing the Gaussian distribution image contrast. Since the fluorescence imaging camera in the laser light source speckle measurement method is directly used as the receiving screen of the laser speckle, the influence of the projection screen, the observation angle and position of the camera relative to the screen and other factors are eliminated on the basis of ensuring the consistency of the test, so this measurement method It can truly and reliably measure the speckle contrast of the laser light source, and has high measurement accuracy.

Description

technical field [0001] The invention relates to the technical field of laser imaging, in particular to a laser light source speckle measurement method, a speckle suppression device and a parameter optimization method thereof. Background technique [0002] Gene sequencing refers to the analysis of the base sequence of a specific DNA fragment, that is, the arrangement of adenine (A), thymine (T), cytosine (C) and guanine (G). At present, the method of fluorescent labeling is generally used for gene sequencing. The gene sequencer uses an optical system containing a microscope objective to excite and collect the fluorescent signals emitted by the fluorescent markers on the gene sequencing chip. High-throughput gene sequencing imaging systems usually use lasers to excite fluorescent marker areas within the field of view of the objective lens on the sequencing chip, and the excited fluorescent energy is collected by a large-target camera. The laser light source of a gene sequence...

AI Technical Summary

Problems solved by technology

[0005] Existing laser speckle suppression and speckle contrast measurement technologies and devices are aimed at laser display systems, and are limited by factors such as different optical systems, display surface characteristics, camera lenses, camera positions, exposure time, etc., and the test results are mainly Assess the impact of speckle on the image observed by human eyes. The measurement result deviates greatly from the actual situation, which obviously cannot meet the needs of the evaluation of the speckle suppression effect of the light source of the gene sequencer.

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