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Colon cancer diagnostic marker and application thereof

A diagnostic marker, colon cancer technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of high difficulty in sample acquisition, unsuitable for early screening of diseases, etc., achieve good clinical application value, improve detection convenience, The effect of promoting standardization

Active Publication Date: 2019-06-14
CHINA PHARM UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] At present, most metabolomics research samples are serum or tissue sample level tests, which are difficult to obtain and are not suitable for early screening of diseases

Method used

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  • Colon cancer diagnostic marker and application thereof
  • Colon cancer diagnostic marker and application thereof
  • Colon cancer diagnostic marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Screening of urine differential metabolites between colon cancer patients and healthy individuals

[0025] 1. Collection and grouping of urine samples:

[0026] According to the pre-experimental design, the biomedical engineering laboratory of China Pharmaceutical University collected urine samples from 32 cases of colon cancer patients and 58 cases of healthy people, according to standardized and reasonable operation procedures, strictly followed the relevant scientific research ethics requirements and Volunteers gave informed consent and informed. 10 mL of mid-morning urine from volunteers was collected and immediately divided into packages (0.5 mL per tube) and stored in a -80°C refrigerator.

[0027] 2. Main reagents and instruments

[0028] Chromatographically pure reagents acetonitrile, formic acid, and methanol were purchased from Merck, Germany, and pseudouridine, N 2 ,N 2 -Dimethylguanosine, Kynurenine, Valine, N-Acetyl-L-Valine, N-Acetyl-L-Glutamic Acid, 5...

Embodiment 2

[0052] Construct a ROC curve to compare the ability of 16 markers to distinguish colon cancer patients from healthy people

[0053] Binary logistic regression was performed using SPSS software, the level of each metabolic marker was set as a covariate, the group was set as a dependent variable, and the regression method was direct entry. After all the variables were introduced into the regression model, the regression equation of the optimal Logistic prediction model of disease occurrence was obtained according to the regression coefficient (B). After obtaining the tumor occurrence probability P value of each sample by the Logistic regression formula, the receiver operating characteristic analysis was continued on the probability P value. Select different values ​​in the range of 0-1 as the critical P value, compare the P value calculated by the above test sample with the critical P value, and calculate the predictive sensitivity and specificity for tumor occurrence under the ...

Embodiment 3

[0062] Application of 16 Markers in Colon Cancer Screening

[0063] In addition, 20 cases of urine samples from patients diagnosed with colon cancer and 20 cases of normal people were selected, and the samples were prepared according to the method described in Example 1, and the metabolites of the urine samples were qualitatively analyzed by LC-MC. Quantitative determination. In this implementation case, 16 marker combinations with the best diagnostic performance were selected. The quantitative data of 16 kinds of markers are brought into the regression equation of the Logistic prediction optimal model obtained by Example 2, and the negative and positive samples are judged according to the calculated Logit (P) value and the size of the cutoff value, if Logit (P ) value is higher than the cut-off value, it is judged as positive (colon cancer), otherwise, it is judged as negative (normal person). The diagnostic results of a total of 40 samples are shown in Table 3.

[0064] T...

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Abstract

The invention discloses a colon cancer diagnostic marker and application thereof. The colon cancer diagnostic marker is urine metabolites and includes at least one of pseudouridine, N2, N2-dimethylguanosine, kynurenine, valine, N-acetylL-valine, N-acetyl-L-glutamic acid, 5-hydroxytryptophan, alpha, beta-didehydrotryptophan, hippuric acid, aspartyl-phenylalanine, taurine, pantothenic acid, 2,3-pyridinedicarboxylic acid, 4-pyridoxic acid, L-carnitine and xanthine. Based on the marker, the invention further provides a colon cancer detection kit which can effectively distinguish colon cancer patients from healthy people and has high accuracy, sensitivity and specificity. A sample selected by the colon cancer diagnostic marker and the colon cancer detection kit is a urine sample, preferably themiddle part of fasting morning urine, invasive samples such as tissues and blood are avoided, and the colon cancer diagnostic marker and the colon cancer detection kit have higher patient complianceand clinical application value.

Description

technical field [0001] The invention belongs to the technical field of biological detection, and in particular relates to a colon cancer diagnostic marker and its application. Background technique [0002] Colon cancer is a common malignant tumor of the digestive tract. It occurs at the junction of the rectum and the sigmoid colon. The incidence rate is the highest in the 40-50 age group. It is the most common malignant tumor in developed countries such as Western Europe and North America. It is also the nine most common malignant tumors in my country. One of the malignant tumors. In the past 30 years, the incidence of colon cancer in my country has been on the rise. The incidence of colon cancer is related to social environment, lifestyle, especially high-fat and low-fiber diet, and lack of physical activity. During the occurrence and development of colon cancer, tumor cells proliferate indefinitely, and obtain a large amount of raw materials to meet their own growth by ch...

Claims

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Application Information

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IPC IPC(8): G01N33/574G01N30/02
Inventor 顾月清李萍韩武娟吕丽伟
Owner CHINA PHARM UNIV
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