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Detection method of dopamine concentration

A detection method and technology of dopamine, applied in nano-carbon, graphene, material electrochemical variables, etc., can solve the problems of high cost, application limitation, poor stability of Pt catalyst, and achieve improved electron transfer rate, low detection limit, The effect of improving stability

Active Publication Date: 2021-01-22
重庆金域医学检验所有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0003] In the field of electrochemical analysis, both Au and Pt have good electrocatalytic properties, but their application is limited due to the high cost and poor stability of Pt catalysts.

Method used

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  • Detection method of dopamine concentration
  • Detection method of dopamine concentration

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] A method for detecting dopamine concentration, comprising:

[0030] Step 1, preparing aminated reduced graphene oxide and Pt-Au bimetallic modified glassy carbon electrode;

[0031] Step 2, preparing PBS buffer solutions of different concentrations of dopamine;

[0032] Step 3. Use the prepared aminated reduced graphene oxide and Pt-Au bimetallic modified glassy carbon electrode as the working electrode to form a three-electrode system, wherein the auxiliary electrode is a Pt electrode, and the reference electrode is an Ag / AgCl electrode. The three-electrode system is put into the PBS buffer solution of dopamine in turn, and the PBS buffer solution of dopamine is detected by differential pulse voltammetry, and the differential pulse voltammetry curves corresponding to the PBS buffer solutions of different concentrations of dopamine are obtained, and established The relationship equation between dopamine concentration and current intensity;

[0033]Step 4, adding the d...

Embodiment 2

[0041] On the basis of Example 1, in step b, before modifying the pretreated glassy carbon electrode with amination reduction graphene oxide, it also includes dropping 5 μL of cerium / dioxide on the surface of the pretreated glassy carbon electrode with a pipette gun. Silicon dispersion, placed in a GJ-1A type infrared rapid drying oven to dry for 20 minutes and then taken out, cooled to room temperature, the preparation method of the cerium / silicon dioxide dispersion is: dissolve 0.5gCe(NO3)3·6H2O in 100ml of deionized water, then add 25g of nano silicon dioxide, after fully stirring, let it stand for 24h, then transfer to a vacuum drying oven, dry at 100°C for 14h, then transfer to a muffle furnace, at a heating rate of 15°C / Raise the temperature to 300°C for 10 minutes, then raise the temperature to 500°C at a heating rate of 5°C / min, and hold for 2 hours. After cooling, take it out, dissolve it in 50ml of deionized water, and process it ultrasonically for 10 minutes.

[00...

Embodiment 3

[0044] On the basis of Example 2, it also includes, after step c, putting the aminated reduced graphene oxide and the Pt-Au bimetallic modified glassy carbon electrode into a magnetizer for magnetization treatment, specifically: first place the The aminated reduced graphene oxide and Pt-Au bimetallic modified glassy carbon electrodes were treated at a magnetic field strength of 10T for 10 minutes, then at a magnetic field strength of 14T for 15 minutes, and finally at a magnetic field strength of 20T for 15 minutes.

[0045] The detection limit of dopamine for the aminated reduced graphene oxide and Pt-Au bimetallic modified glassy carbon electrode prepared in this example is 4.7×10 -8 mol / L.

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Abstract

The invention discloses a method for detecting the concentration of dopamine. The method comprises the following steps: preparing an amination-reduced graphene oxide and Pt-Au bimetallic modified glassy carbon electrode; preparing PBS buffer solutions of different concentrations of dopamine; constructing a three-electrode system by taking the prepared amination-reduced graphene oxide and Pt-Au bimetallic modified glassy carbon electrode as a working electrode, detecting the PBS buffer solutions of dopamine by differential pulse voltammetry to obtain differential pulse voltammetry curves corresponding to the PBS buffer solutions of different concentrations of dopamine, and establishing a relationship equation between the concentration of dopamine and current intensity; and determining the differential pulse voltammetry curves of a buffer solution to be measured and corresponding current intensity, and deducing the concentration of dopamine in the buffer solution to be measured accordingto the relationship equation between dopamine concentration and current intensity. The method has the advantages of easiness in operation, high sensitivity and low detection limit.

Description

technical field [0001] The invention relates to the technical field of dopamine detection. More specifically, the present invention relates to a method for detecting dopamine concentration. Background technique [0002] At present, the methods for detecting dopamine mainly include liquid chromatography, chemiluminescence, capillary electrophoresis, fluorescence technology, mass spectrometry and spectrophotometry, etc. Although these technologies are widely used, the instruments used are expensive, the consumption of reagents is large, and the analysis time is long Long, can not meet the needs of on-site rapid detection. Dopamine is electrochemically active and easily oxidized, so it can be detected by electrochemical methods, such as the method for detecting the concentration of dopamine in solution previously disclosed by our research group (application number: 201710765619.5), which uses carbon dot solution and amination reduction oxidation The graphene-modified glassy c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/30G01N27/48C25D3/56C25D5/54C01B32/182
Inventor 肖琦黄珊李家文金晓宇
Owner 重庆金域医学检验所有限公司
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