Application of lysozyme two-dimensional nano-film as antibacterial material

A two-dimensional nano, antibacterial material technology, applied in applications, fungicides, chemicals for biological control, etc., can solve the problems of difficult to form a continuous film structure, poor stability, low mechanical strength, etc. Spectrum and efficient antibacterial properties, good antibacterial efficiency, easy operation effect

Active Publication Date: 2019-06-07
SHAANXI NORMAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Of course, as a water-soluble protein molecule, lysozyme has the common disadvantages of protein molecules, such as low mechanical strength, difficulty in forming a continuous film structure on a solid surface, poor stability, and rapid dissolution in aqueous solution, etc.
These characteristics limit its application to some extent

Method used

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  • Application of lysozyme two-dimensional nano-film as antibacterial material
  • Application of lysozyme two-dimensional nano-film as antibacterial material
  • Application of lysozyme two-dimensional nano-film as antibacterial material

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] The application of lysozyme two-dimensional nano film as an antibacterial material is as follows:

[0026] Add 0.1433g of tris(2-carboxyethyl)phosphine to 10mL of 10mmol / L 4-hydroxyethylpiperazineethanesulfonic acid buffer solution with a pH value of 7.4, adjust the pH value to 6.4 with NaOH, and prepare a 50mmol / L tris 4-hydroxyethylpiperazineethanesulfonic acid buffer solution of (2-carboxyethyl)phosphine; add 40mg of lysozyme to 10mL10mmol / L pH value of 7.4 in the 4-hydroxyethylpiperazineethanesulfonic acid buffer solution to prepare 4mg / mL 4-hydroxyethylpiperazineethanesulfonic acid buffer solution of lysozyme; take 10mL50mmol / L tris(2-carboxyethyl)phosphine 4-hydroxyethylpiperazineethanesulfonic acid buffer solution and 10mL4mg / mL lysozyme Mix the 4-hydroxyethylpiperazineethanesulfonic acid buffer solution of the enzyme evenly, let it stand at room temperature for 50 minutes, and form a thin film on the surface of the mixed solution, that is, a two-dimensional nano...

Embodiment 2

[0028] The application of lysozyme two-dimensional nano film as an antibacterial material is as follows:

[0029] Add 0.1433g of tris(2-carboxyethyl)phosphine into 10mL of 10mmol / L tris(2-carboxyethyl) buffer solution with a pH value of 7.4, adjust the pH value to 6.4 with NaOH, and prepare 50mmol / L of tris(2-carboxyethyl) base) tris buffered solution of phosphine; 40 mg lysozyme was added into 10 mL of 10 mmol / L pH value of 7.4 tris buffered solution to prepare 4 mg / mL tris of lysozyme Buffer solution; mix 10mL of 50mmol / L tris (2-carboxyethyl) phosphine tris buffer solution with 10mL 4mg / mL lysozyme tris buffer solution, mix the medical plastic catheter Insert it into the mixed liquid, let it stand at room temperature for 50 minutes, and directly form a layer of self-assembled film on the inner and outer surfaces of the medical plastic catheter (see figure 1 ), that is, a two-dimensional nanofilm of lysozyme, which plays the role of antibacterial protection.

Embodiment 3

[0031] The application of lysozyme two-dimensional nano film as an antibacterial material is as follows:

[0032] The preparation method of lysozyme two-dimensional nano-film in the present embodiment is the same as embodiment 1, then uses agarose gel as medium to transfer lysozyme two-dimensional nano-film to the scalpel surface (see figure 2 ), play the role of antibacterial protection.

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Abstract

The invention discloses an application of a lysozyme two-dimensional nano-film as an antibacterial material. According to the application, the lysozyme two-dimensional nano-film serves as the antibacterial material, the lysozyme two-dimensional nano-film is formed on the surface of a substrate through in-situ growth only during the preparation of the lysozyme two-dimensional nano-film, or the prepared lysozyme two-dimensional nano-film is directly adhered to the surface of the substrate and can be formed on surfaces of substrates of various shapes through in-situ growth or adherence. Accordingto the application, the lysozyme two-dimensional nano-film serves as the antibacterial material and has broad-spectrum and efficient antibacterial performance, an antibacterial spectrum can be extended to three species of bacteria, i.e., gram-positive bacteria, gram-negative bacteria and fungi from category I bacteria of Classic gram-positive bacteria, the lysozyme two-dimensional nano-film presents good antibacterial efficiency to the three species of bacteria, and the antibacterial efficiency basically can reach 90% or more in 2 hours; the lysozyme two-dimensional nano-film has good adhesivity to substrates to be protected and is free of toxicity and adsorptivity to cells; and thus, the lysozyme two-dimensional nano-film has good antifouling properties to the cells and can serve as an ideal antibacterial food packaging material and a medical application program material.

Description

[0001] This application is a divisional application of Chinese Patent Application No. 201510822799.7. The filing date of the original application was November 24, 2015, and the title of the invention was "Application of lysozyme two-dimensional nano-film as an antibacterial material". technical field [0002] The invention belongs to the technical field of antibacterial materials, and in particular relates to the application of a two-dimensional nano film of lysozyme as an antibacterial material. Background technique [0003] Imparting an antimicrobial property is very important in a variety of fields including packaging, textile industry, medicine, water purification and health care. Antibacterial composite materials are widely used due to their excellent comprehensive properties. This composite antibacterial material can assemble different types and functional substances according to different needs. These characteristics endow composite materials with more and newer funct...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N63/00A01N25/34A01P1/00A01P3/00
CPCA01N25/34A01N63/00
Inventor 杨鹏
Owner SHAANXI NORMAL UNIV
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