Method for screening functional genes of edible and medicinal fungi by combining ATAC-seq with RNA-seq

Abstract

The invention discloses a method for screening functional genes of edible and medicinal fungi by combining ATAC-seq with RNA-seq. The method comprises the following steps: testing mycelia of the edible and medicinal fungi at a mycelium culture stage before and after induction of environmental factors and an open chromatin area of primordium after primordium differentiation by induction of environmental factors with an ATAC-seq method and analyzing coding genes of the open area, testing mycelia of the edible and medicinal fungi at a mycelium culture stage and an mRNA sequence after primordium differentiation with an RNA-seq method, searching common differential genes of gene sequences obtained by the ATAC-seq method and the RNA-seq method by use of a Venn map, and finally, searching key functional gene sequences influencing primordium differentiation of the edible and medicinal fungi in the common differential genes by biological analysis.

Description

technical field [0001] The invention belongs to the technical field of biology, and in particular relates to a method for screening functional genes of edible and medicinal fungi by combining ATAC-seq and RNA-seq. Background technique [0002] Edible and medicinal fungi are a large class of higher fungi with large fruiting bodies, commonly known as mushrooms or mushrooms. At present, there are more than 1,000 kinds of edible fungi in my country, most of which belong to basidiomycetes, and a small part of them belong to ascomycetes. Edible and medicinal fungi are rich in polysaccharides, proteins, triterpenoids, polypeptides, dietary fiber, taurine, mannitol, unsaturated fatty acids, adenosine, lactone, minerals and vitamins, etc. With the characteristics of low fat, low sugar and high protein, it is recommended by nutritionists as one of the top ten healthy foods, and has huge market prospects and development potential. Among them, hydrangea is a rare edible and medicinal f...

AI Technical Summary

Problems solved by technology

The genetic screening of edible and medicinal fungi is limited to the use of single technologies such as transcriptome sequencing and proteomics, but the method of screening differentially expressed genes in the process of primordium differentiation of edible and medicinal fungi through such a single technology usually fails. Finding a large number of differential genes leads to a heavy workload for subsequent gene function identification and verification, and the specificity of specific genes identified by a single technology is low, and more false positives are prone to occur, making it impossible to efficiently screen out edible and medicinal fungi Key genes in primordia differentiation stages

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