Microbial fermented preparation prepared from procambarus clarkia processing wastes as well as preparation method and application of microbial fermented preparation
A microbial fermentation and waste technology, applied in the field of agriculture and animal husbandry, can solve the problems of harsh microbial fermentation conditions, additives, secondary pollution, etc., achieve the effect of eliminating bitterness and fishy smell, wide application range, and high nutritional value
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Embodiment 1
[0031] A preparation method of crayfish processing waste microbial fermentation preparation, comprising the following steps:
[0032] (1) Place crayfish processing waste (such as shrimp head and shrimp shell) in an oven, dry at a temperature of 70°C until constant weight, and crush to make crayfish processing waste dry powder.
[0033] (2) Preparation of medium:
[0034] Preparation of LB liquid medium: Dissolve 1.0g of peptone, 0.5g of yeast extract and 1.5g of sodium chloride in water, add water to 100mL, and sterilize.
[0035] Preparation of MRS liquid medium: 1.0g peptone, 1.0g beef extract, 0.5g yeast extract, 2.0g glucose, 0.2g dipotassium hydrogen phosphate, 0.025g manganese sulfate, 0.2g diamine citrate, 0.058g sulfuric acid and 0.1 Dissolve mL Tween in water, add water to make up to 100mL, adjust pH value to 6.4, and sterilize.
[0036] Preparation of YPD liquid medium: Dissolve 2.0 g of glucose, 1.0 g of peptone and 0.5 g of yeast extract in water, add water to ma...
Embodiment 2
[0044] A method for preparing a microbial fermentation preparation of crayfish processing waste, which is basically the same as the preparation method in Example 1, except that in step (4) of Example 2, the fermentation temperature is 30°C, and the total amount of inoculation is 24 %, the fermentation time is 48h.
[0045] Take the crayfish processing waste microbial fermentation preparation and crayfish processing waste (i.e. the raw materials used in the step (1) of Example 1) obtained in Example 1 and Example 2, and use a high performance liquid chromatography HPLC instrument (CBM- 10A VP Plus, Japan Shimadzu Co., Ltd.) to detect the content of amino acids in these three samples, that is, the content of amino acids in the microbial fermentation preparation of crayfish processing waste.
[0046] The content of amino acids in the crayfish processing waste microbial fermentation preparation prepared in Example 1 of the present invention is 42.506 μg / g, while the content of ami...
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