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Microbial fermented preparation prepared from procambarus clarkia processing wastes as well as preparation method and application of microbial fermented preparation

A microbial fermentation and waste technology, applied in the field of agriculture and animal husbandry, can solve the problems of harsh microbial fermentation conditions, additives, secondary pollution, etc., achieve the effect of eliminating bitterness and fishy smell, wide application range, and high nutritional value

Inactive Publication Date: 2019-06-04
CHANGSHA UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] To sum up, there are the following defects in the prior art: (1) The prior art mainly extracts macromolecular proteins and astaxanthin from crayfish processing waste, but does not involve the method of extracting small molecular amino acids; (2) ) There are still the following problems in the existing treatment process: exogenous nutrients need to be added; pH value adjustment, sterilization or heat preservation treatment is required before fermentation; the fermentation conditions required by the microorganisms used are harsh, and the existence of the above problems increases the treatment process and treatment cost, there is a risk of secondary pollution, and it is not conducive to industrial production, which greatly limits the resource reuse of crayfish processing waste

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A preparation method of crayfish processing waste microbial fermentation preparation, comprising the following steps:

[0032] (1) Place crayfish processing waste (such as shrimp head and shrimp shell) in an oven, dry at a temperature of 70°C until constant weight, and crush to make crayfish processing waste dry powder.

[0033] (2) Preparation of medium:

[0034] Preparation of LB liquid medium: Dissolve 1.0g of peptone, 0.5g of yeast extract and 1.5g of sodium chloride in water, add water to 100mL, and sterilize.

[0035] Preparation of MRS liquid medium: 1.0g peptone, 1.0g beef extract, 0.5g yeast extract, 2.0g glucose, 0.2g dipotassium hydrogen phosphate, 0.025g manganese sulfate, 0.2g diamine citrate, 0.058g sulfuric acid and 0.1 Dissolve mL Tween in water, add water to make up to 100mL, adjust pH value to 6.4, and sterilize.

[0036] Preparation of YPD liquid medium: Dissolve 2.0 g of glucose, 1.0 g of peptone and 0.5 g of yeast extract in water, add water to ma...

Embodiment 2

[0044] A method for preparing a microbial fermentation preparation of crayfish processing waste, which is basically the same as the preparation method in Example 1, except that in step (4) of Example 2, the fermentation temperature is 30°C, and the total amount of inoculation is 24 %, the fermentation time is 48h.

[0045] Take the crayfish processing waste microbial fermentation preparation and crayfish processing waste (i.e. the raw materials used in the step (1) of Example 1) obtained in Example 1 and Example 2, and use a high performance liquid chromatography HPLC instrument (CBM- 10A VP Plus, Japan Shimadzu Co., Ltd.) to detect the content of amino acids in these three samples, that is, the content of amino acids in the microbial fermentation preparation of crayfish processing waste.

[0046] The content of amino acids in the crayfish processing waste microbial fermentation preparation prepared in Example 1 of the present invention is 42.506 μg / g, while the content of ami...

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PUM

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Abstract

The invention discloses a microbial fermented preparation prepared from procambarus clarkia processing wastes as well as a preparation method and an application of the microbial fermented preparation.The microbial fermented preparation prepared from the procambarus clarkia processing wastes is prepared from raw materials of procambarus clarkia processing wastes by synergistic fermentation of lactobacillus, bacillus subtilis YS-45 and saccharomyces S-78. The microbial fermented preparation prepared from the procambarus clarkia processing wastes contains rich multiple probiotics and amino acids, has the content of amino acids up to 56.274 mu g / g and has the advantages that the nutrient value is higher, the application range is wider and economic benefits are greater. The preparation methodof the microbial fermented preparation has the advantages that the processing technology is simple, convenient to operate and low in processing cost, can be used for mass preparation and facilitates industrial production. The microbial fermented preparation prepared from procambarus clarkia processing wastes can be widely applied to the feed field as a raw material and has better economic values and application values.

Description

technical field [0001] The invention belongs to the technical field of agriculture and animal husbandry, and relates to a microbial fermentation preparation of crayfish processing waste, a preparation method and application thereof. Background technique [0002] Crayfish, also known as Procambarus clarkii ( Procambarus clarkia ), native to northern Mexico and southern United States, was introduced into my country in the late 1930s and has become one of the most important aquatic economic shrimps in my country. As of 2016, the total output of crayfish in the country was 899,100 tons, with an annual output value of 56.41 billion yuan. In recent years, due to the continuous expansion of the scale of crayfish farming and the continuous increase in output, a large amount of waste of shrimp heads and shrimp shells has been produced in the process of crayfish processing. These processing by-products are rich in nutrients, but most of them are discarded and rarely used. This not o...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12N1/16A23K10/18C12R1/225C12R1/125
Inventor 瞿符发刘甜婷高倩婷刘臻唐建洲陈圆易翦涵吴圳达
Owner CHANGSHA UNIVERSITY
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