Method for separating and purifying FMD inactivated virus antigen by applying simulated flow bed
A technology for simulating a fluidized bed and viral antigens, which is applied in the preparation methods of peptides, chemical instruments and methods, viral peptides, etc. It can solve the problems of long purification process time, high packing requirements, and low packing capacity, and shorten the separation time. The effect of purification time, high degree of continuity, and improved preparation efficiency
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Embodiment 1
[0045] The present embodiment provides a kind of method of applying simulated fluidized bed to separate and purify FMD inactivated virus antigen, comprising the following steps:
[0046] S1, balance the chromatographic column of the simulated fluidized bed with an equilibrium buffer solution;
[0047] S2. Load the FMD inactivated virus antigen sample to the simulated fluidized bed chromatography column. During the sample loading process, the chromatographic column can be cleaned of antigen sample impurities and sample desorption and elution, and the cleaning regeneration and balance of the chromatographic packing Liquid equilibration, the purified sample is collected during continuous desorption and elution.
[0048] The concrete steps of described S2 are:
[0049] A. Load the FMD inactivated virus antigen sample to the simulated fluidized bed chromatographic column, detect the effluent of each chromatographic column during the sample loading process, and detect that FMD is k...
Embodiment 2
[0062] The present embodiment provides a kind of method of applying simulated fluidized bed to separate and purify FMD inactivated virus antigen, comprising the following steps:
[0063] S1, balance the chromatographic column of the simulated fluidized bed with an equilibrium buffer solution;
[0064] S2. Load the FMD inactivated virus antigen sample to the simulated fluidized bed chromatography column. During the sample loading process, the chromatographic column can be cleaned of antigen sample impurities and sample desorption and elution, and the cleaning regeneration and balance of the chromatographic packing Liquid equilibration, the purified sample is collected during continuous desorption and elution.
[0065] The concrete steps of described S2 are:
[0066] A. Load the FMD inactivated virus antigen sample to the simulated fluidized bed chromatographic column, detect the effluent of each chromatographic column during the sample loading process, and detect that FMD is k...
Embodiment 3
[0079] The present embodiment provides a kind of method of applying simulated fluidized bed to separate and purify FMD inactivated virus antigen, comprising the following steps:
[0080] S1, balance the chromatographic column of the simulated fluidized bed with an equilibrium buffer solution;
[0081] S2. Load the FMD inactivated virus antigen sample to the simulated fluidized bed chromatography column. During the sample loading process, the chromatographic column can be cleaned of antigen sample impurities and sample desorption and elution, and the cleaning regeneration and balance of the chromatographic packing Liquid equilibration, the purified sample is collected during continuous desorption and elution.
[0082] The concrete steps of described S2 are:
[0083] A. Load the FMD inactivated virus antigen sample to the simulated fluidized bed chromatographic column, detect the effluent of each chromatographic column during the sample loading process, and detect that FMD is k...
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