Method and device for continuously obtaining plant secondary metabolic products through tissue culture

Abstract

The invention discloses a method and device for continuously obtaining plant secondary metabolic products through tissue culture, and particularly relates to the field of plant biology. The device comprises a box shell. A detachable net frame mechanism and a precise dosage mechanism are arranged in the box shell. The precise dosage mechanism is arranged on the top of the detachable net frame mechanism. The detachable net frame mechanism comprises an annular fixing plate. A filter screen is fixedly arranged on the inner side of the annular fixing plate. A plurality of placement holes run through the filter screen. A bearing hopper is arranged inside each placement hole. A culture hopper is arranged inside each bearing hopper. A plurality of through holes run through the bearing hoppers andthe culture hoppers. Placed plant tissue and organs can be in a scattered state; compared with the prior art, the placement difficulty is low, the placement effect is precise, there is no need to makeadjustment multiple times, labor consumption is effectively reduced, meanwhile it can be ensured that the plant tissue and organs in the culture hoppers can be effectively watered, and the good growth of the plant tissue and organs is ensured.

Description

technical field [0001] The invention relates to the field of plant biotechnology, more specifically, the invention relates to a method and a device for continuously obtaining plant secondary metabolites through tissue culture. Background technique [0002] Secondary metabolites are non-essential small-molecule organic compounds produced by secondary metabolism in a class of cell life activities or in the normal operation of plant growth and development, and their production and distribution are usually specific to species, organs, tissues, and growth and development stages , can be divided into seven categories: phenylpropanoids, quinones, flavonoids, tannins, terpenes, steroids and their glycosides, and alkaloids. Secondary metabolites in medicinal plants are the key to their medicinal effects Element. [0003] The invention patent of patent application publication number CN103120124B discloses a method for continuously obtaining plant secondary metabolites through tissue ...

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Problems solved by technology

[0004] However, in actual use, there are still some shortcomings. For example, in actual implementation, plant tissues and organs need to be placed on a multi-layer culture grid. Placement, the actual operation is difficult, and when it is used in industrial production, it needs to consume more manpower. At the same time, when the equipment is used to irrigate plant tissues and organs, it cannot perform more uniform irrigation, which will cause damage to the growth of plant tissues and organs. influences

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