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Method of quickly detecting protein concentration in traditional Chinese medicine extract and application of method

A protein concentration and extraction solution technology, applied in Raman scattering, material excitation analysis, etc., can solve the problems of real-time release of unfavorable pharmaceutical intermediates, non-compliance with green manufacturing, slow processing speed, etc., to achieve short measurement time and safety assurance , the effect of easy operation

Inactive Publication Date: 2019-05-24
贵州拜特制药有限公司
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Problems solved by technology

[0005] The traditional protein determination method is complex in operation, slow in processing speed, requires pretreatment and additional reagents and solvents, does not conform to the concept of green manufacturing, and is not conducive to the realization of real-time release of pharmaceutical intermediates

Method used

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  • Method of quickly detecting protein concentration in traditional Chinese medicine extract and application of method
  • Method of quickly detecting protein concentration in traditional Chinese medicine extract and application of method
  • Method of quickly detecting protein concentration in traditional Chinese medicine extract and application of method

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Embodiment Construction

[0073] The present invention will be further described in conjunction with the following examples, but it is not a basis for limiting the present invention.

[0074] Examples. The method for quickly detecting the protein concentration in the Chinese medicine extract is to measure the Raman spectroscopy data of the protein concentration of the Chinese medicine extract to be tested, and calculate the protein concentration by using the constructed calibration model.

[0075] The method is to collect a calibration sample of traditional Chinese medicine extract, determine the Raman spectrum data of the calibration sample, and determine the concentration of the protein in the calibration sample, and establish a calibration model based on the concentration of the protein in the calibration sample and the corresponding Raman spectrum data. Input the Raman spectrum data of the extract of Chinese medicine to be tested into the constructed calibration model, and then the protein concentration...

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Abstract

The invention discloses a method of quickly detecting protein concentration in traditional Chinese medicine extract and application of the method. The method comprises: (a) collecting a correcting setsample; (b) detecting protein concentration in the correcting set sample by means of Coomassie brilliant blue process; (c) measuring the correcting set sample by means of Raman spectrum; (d) establishing a correcting model; (e) detecting a sample under test. The method of quickly detecting protein concentration in traditional Chinese medicine extract based on Raman spectrum provided herein is simple and quick to perform; compared with traditional protein measuring methods (chemical rendering processes), the method herein has no need for preparing a followup standard curve, has a shorter measuring time, has no need for using various chemical reagents and solvents and has good environmental friendliness. Raman characteristic spectrum of Salvia miltiorrhiza extract in an embodiment is well correlated with the protein concentration, and a measurement error is less than 10%. In the method, the mass of a traditional Chinese medicine injection intermediate in mass index control is monitoredin real time, so that safety and validity are guaranteed for a traditional Chinese medicine injection.

Description

Technical field [0001] The invention relates to a method and application for monitoring the quality of the production process, in particular to the rapid detection of protein concentration in a traditional Chinese medicine extract. Background technique [0002] Protein is a common type of impurity in Chinese medicine injection, and the determination of its content is of great significance to control the quality of Chinese medicine injection and ensure the safety of Chinese medicine injection. The protein quantitative analysis methods listed in the "Chinese Pharmacopoeia" include Kjeldahl method, Folin's reagent method, biuret method, Coomassie brilliant blue method, 2,2'-biquinoline-4, dicarboxylic acid ( BCA) method and ultraviolet-visible spectrophotometry. The above methods are applicable to different objects. The ultraviolet-visible spectrophotometry is only suitable for the detection of purified proteins. The Kjeldahl method is less sensitive and has more interferences in c...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
Inventor 瞿海斌潘坚扬赵芳薛捷李白玲李辉
Owner 贵州拜特制药有限公司
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