A kind of preparation method of fungal mycelia fine powder

A mycelium and fungal technology, which is applied in the field of preparation of fungal mycelium fine powder, can solve the problems of fungal fruiting body reduction in activity, increase in vacuum drying time, incomplete sterilization and disinfection, etc., and achieve good economic and social effects, Bright color and low price effect

Active Publication Date: 2021-06-04
NORTHWEST UNIV
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Problems solved by technology

However, if the concentration of sodium hypochlorite is too low, it will cause incomplete sterilization and easy contamination, and if the concentration is too high, the activity of fungal fruit bodies will be reduced or even lose biological activity.
Therefore, although the growth rate of fungi in the conventional liquid culture method is better than that in the solid culture method, the application of the cultivation of some strains in the actual industry is not ideal due to the lack of stability.
[0004] For the separation of mycelia and fermentation broth, the current laboratory methods include the following: centrifugation, filtration, and suction filtration. Through comparative experiments on several methods in the laboratory, the following results have been obtained: centrifugation Although solid-liquid separation can be achieved, the mycelium still contains a large amount of liquid at this time, which will increase the time of vacuum drying; the filtration method uses gauze to separate the mycelial fermentation liquid, which can also achieve the purpose of solid-liquid separation, but due to The fermentation liquid itself is relatively viscous, it will take more time, and it will also increase the drying time; the suction filtration method uses the pressure generated by the vacuum pump to separate the mycelia fermentation liquid, which has the advantages of fast separation speed and low water content of mycelia. characteristics, it is an ideal method

Method used

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  • A kind of preparation method of fungal mycelia fine powder

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Embodiment Construction

[0030] Take a kind of new strain Fibularhizoctonia sp. that the applicant discovers below as an example, introduce the preparation method of this fungus mycelia fine powder in detail:

[0031] (1) Activation of strains and preparation of mycelium mother liquor

[0032] The collected fungal sclerotia was washed several times with 1% (v / v) NaClO solution, soaked for 2-3min each time, after pouring off the waste liquid, washed off its surface residual liquid with sterile water, and then inoculated on Place the prepared slant culture medium at 24-27°C for 3-4 days. After the cultivation is complete, select the strains with better growth and store them at 4°C for later use. Wherein, the slant culture medium can be prepared by conventional methods.

[0033] Use a sterile inoculation needle to inoculate the preserved mycelium species into PDA liquid medium (agar may not be added) in an aseptic operating table, put it into a shaking table at 24-27° C. for 3-4 days, and obtain the myc...

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Abstract

The invention provides a method for preparing fine fungal mycelium powder, which solves the problems of processing and preserving the mycelium obtained from liquid culture. The method is carried out according to the following steps: 1) obtaining mycelium through liquid expansion culture; 2) subjecting the liquid mycelium to suction filtration to obtain a fungus cake; 3) placing the fungus cake in vacuum drying equipment or microwave equipment Drying treatment; 4) After the bacteria cake is dried, it is pulverized, sieved and preserved. The fungal mycelium fine powder prepared by the method has bright color and no peculiar smell, and the operation method is simple and easy, and the production cost is low.

Description

technical field [0001] The invention relates to a preparation method of fungal mycelium fine powder. Background technique [0002] Making fungal mycelium into mycelium fine powder is an effective way to increase its preservation time. Among them, the expanded cultivation of fungi is the basic link, and the separation and drying of mycelia from the culture system are the final output links, and the design of this link is closely related to the culture system. [0003] Conventional liquid culture methods mostly directly inoculate fungal sclerotia in liquid medium, although 1% sodium hypochlorite solution will be used for disinfection and sterilization before inoculation. However, if the concentration of sodium hypochlorite is too low, it will cause incomplete sterilization and easy contamination, and if the concentration is too high, the fungal fruiting bodies will reduce their activity or even lose their biological activity. Therefore, although the growth rate of the fungus...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12N1/04C12R1/64
Inventor 邢连喜张宏贵赵剑
Owner NORTHWEST UNIV
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