Slow virus co-transfection adherent 293T cell device for large-scale cell therapy

Abstract

The invention discloses a slow virus co-transfection adherent 293T cell device for large-scale cell therapy. The device comprises a culture device body, electric heating plates, an incubator, magneticballs, a culture plate, an adherent layer, electromagnets, a first control box and a second control box. A culture bin is arranged on one side of the lower portion of the culture device body, the electric heating plates are arranged on two inner sides of the culture bin, the magnetic balls are arranged at the inner bottom of the incubator, the culture plate is arranged in a groove, the electromagnets are uniformly arranged at the bottom of a cover plate, a fan is mounted on one inner side of the first control box, a controller REX-C900 is arranged on one side of a temperature sensor, and a controller BF-10T is arranged on one side of a timer. The incubator is used for culture, the adherence area of cells is increased by the culture plate, the culture plate is easily taken out, cell growthconditions are met, large-scale culture is facilitated, the magnetic balls are adsorbed by the electromagnets and accordingly move up and down in the incubator, culture solution is stirred, and the cells cannot be injured.

Description

technical field [0001] The invention relates to the technical field of cell adherence culture, in particular to a device for co-transfecting adherent 293T cells with lentivirus for large-scale cell therapy. Background technique [0002] 293T cell is a cell line derived from 293 cells through genetic technology. It is transfected with adenovirus E1A gene and can express SV40 large T antigen. It contains SV40 replication origin and promoter region. It is a very commonly used expression research tool The cells of the source gene are of great significance for the use of gene therapy for diseases, and 293T cells are usually propagated by cell adherent culture. [0003] However, the current cell adherence culture device has a small amount of culture, and the cultured cells are not easy to take out, which is not conducive to large-scale culture. When culturing the cells, stirring is required, which is easy to cause damage to the cultured cells, which is not conducive to the growth ...

AI Technical Summary

Problems solved by technology

[0003] However, the current cell adherence culture device has a small culture capacity, and the cultured cells are not easy to take out, which is not conducive to large-scale culture. When cultivating cells, they need to be stirred, which is easy to cause damage to the cultured cells, which is not conducive to the growth of cells.

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