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Preparation method of composite sustained-release microcapsule preparation for treating ammonia nitrogen in culture water

A technology of slow-release microcapsules and aquaculture water, which is applied in the field of biotechnology and micro-ecological preparations, can solve problems such as weak viability, short action time, and increased ammonia nitrogen in water, and achieve strong resistance, reduced incidence, and high nutritional value. high effect

Active Publication Date: 2019-05-21
NANJING UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The purpose of the present invention is to provide a method for preparing a compound slow-release microcapsule preparation for treating ammonia nitrogen in aquaculture water, aiming at the problem that the ammonia nitrogen in the water body increases during the aquaculture process and affects the survival of aquatic animals.
The microcapsule preparation obtained by this method can solve the problems of poor viability, short action time and weak effect of live bacteria in harsh water environment

Method used

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  • Preparation method of composite sustained-release microcapsule preparation for treating ammonia nitrogen in culture water
  • Preparation method of composite sustained-release microcapsule preparation for treating ammonia nitrogen in culture water
  • Preparation method of composite sustained-release microcapsule preparation for treating ammonia nitrogen in culture water

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Effect test

Embodiment 1

[0041] (1) Activation culture: mix Bacillus cereus suspension, Bacillus subtilis suspension, and Bacillus licheniformis suspension at a bacterial ratio of 1:1:1 and insert them into the activation medium to control the volume of the culture medium The filling volume is 15%, the activation culture temperature is 32°C, the pH is 7.0, and the rotation speed is 160r / min; after 24 hours of activation culture, the seed liquid is obtained; wherein the activation medium components are (g / L): glucose 5.0, peptone 5.0, Beef Extract 2.0, NaCl 5.0.

[0042] (2) Fermentation culture: inoculate the seed solution of step (1) in the fermentation medium with a volume inoculation amount of 5%, the fermentation temperature is 32° C., the pH is 7.0, and the rotating speed is 160 r / min; after 24 hours of fermentation culture, centrifuge The spores were collected and dried; the components of the fermentation medium were (g / L): glucose 5.0, peptone 5.0, beef extract 5.0, NaCl 3.0, MgSO4 7H2O 1.0, K2...

Embodiment 2

[0046] (1) Activation culture: mix Bacillus cereus suspension, Bacillus subtilis suspension, and Bacillus licheniformis suspension at a bacterial ratio of 1:1:2 and then insert them into the activation medium to control the volume of the culture medium The liquid filling volume is 20%, the activation culture temperature is 34°C, the pH is 7.2, and the rotation speed is 180r / min; after the activation culture is 30h, the seed liquid is obtained; wherein the activation medium composition is (g / L): glucose 8.0, peptone 8.0, Beef extract 4.0, NaCl 7.0.

[0047] (2) Fermentation culture: inoculate the seed solution of step (1) in the fermentation medium with a volume inoculation amount of 6%, the fermentation temperature is 35° C., the pH is 7.3, and the rotating speed is 180 r / min; after 30 hours of fermentation culture, centrifuge The spores were collected and dried; the components of the fermentation medium were (g / L): glucose 8.0, peptone 9.0, beef extract 7.0, NaCl 4.0, MgSO4 7...

Embodiment 3

[0051] (1) Activation culture: mix Bacillus cereus suspension, Bacillus subtilis suspension, and Bacillus licheniformis suspension at a bacterial ratio of 1:2:3 and insert them into the activation medium to control the volume of the culture medium The filling volume is 25%, the activation culture temperature is 36°C, the pH is 7.5, and the rotation speed is 200r / min; after 48 hours of activation culture, the seed liquid is obtained; wherein the activation medium composition is (g / L): glucose 10.0, peptone 10.0, Beef extract 5.0, NaCl 10.0.

[0052] (2) Fermentation culture: inoculate the seed solution of step (1) in the fermentation medium with an inoculum volume of 8%, the temperature of the fermentation culture is 37°C, the pH is 7.5, and the rotating speed is 200r / min; after 48h of fermentation culture, centrifuge The spores were collected and dried; the components of the fermentation medium were (g / L): glucose 10.0, peptone 10.0, beef extract 8.0, NaCl 5.0, MgSO4 7H2O 2.0,...

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Abstract

The invention discloses a preparation method of a composite sustained-release microcapsule preparation for treating ammonia nitrogen in culture water. The method is mainly applied to ammonia nitrogentreatment of the water in aquaculture. Spores of bacillus cereus, bacillus subtilis and bacillus licheniformis are used as core materials, and a whey protein and sodium alginate are used as wall materials to prepare double-coated microcapsules with an embedding rate of 90%. The method is used in the aquaculture, the main function is to purify the ammonia nitrogen in the culture water, antagonize pathogens, improve the immunity of cultured aquatic products and prevent disease occurrence, and a use period is more than 50 days.

Description

technical field [0001] The invention relates to the fields of biotechnology and probiotics. In particular, it relates to a preparation method of a compound slow-release microcapsule preparation for treating ammonia nitrogen in aquaculture water. Background technique [0002] In the process of aquaculture, a large amount of surplus bait and excrement of farmed animals accumulate in the water body, which deteriorates the water body environment, including the increase of ammonia nitrogen and nitrite content, and the decrease of dissolved oxygen, which leads to frequent occurrence of farmed animal diseases and reduced economic benefits. Therefore, the control of aquaculture water environment is the top priority of aquaculture. Microecological preparations are live bacterial preparations processed from beneficial microorganisms, which can regulate water quality, antagonize pathogenic bacteria, and improve immunity in aquaculture. Commonly used probiotics mainly include photosyn...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C02F3/34C12N1/20C12N3/00C12R1/085C12R1/10C12R1/125C02F101/16
CPCY02W10/10
Inventor 胡永红范瑜雯杨文革李旭红陈百尧许贺曹洋周翼
Owner NANJING UNIV OF TECH
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