Method for improving stability of N-acetylneuraminic acid aqueous solution and application of method

Abstract

The invention provides a method for improving the stability of a N-acetylneuraminic acid aqueous solution and application of the method. The method comprises the following steps that the pH of the aqueous solution with N-acetylneuraminic acid is adjusted to 5-9; the N-acetylneuraminic acid is obtained through fermentation. By adopting the method for adjusting the pH value of the N-acetylneuraminicacid aqueous solution prepared through fermentation, the stability of the N-acetylneuraminic acid aqueous solution is improved. It is proved through experiments that by adopting the method, the stability of the N-acetylneuraminic acid aqueous solution can be effectively improved, and the method is easy to operate.

Description

technical field [0001] The invention relates to the field of industrialized production, more specifically, to a method for improving the stability of an aqueous solution of N-acetylneuraminic acid and its application. Background technique [0002] N-acetylneuraminic acid is an acidic amino sugar with 9 carbon atoms and a pyranose structure. It can regulate IgG anti-inflammatory activity, enhance and promote infant immunity and brain development, and affect the integrity of nerve cells. The role of permeability and activity is involved in multiple physiological processes such as cell recognition, signal transduction, tumorigenesis, and fertilization, and can be used in the fields of food, cosmetics, and pharmaceuticals. [0003] In industry, N-acetylneuraminic acid is usually produced by fermentation. After fermentation, a series of post-treatments are carried out on the fermentation broth to obtain N-acetylneuraminic acid. A patented preparation of separation, purification a...

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Problems solved by technology

[0003] In industry, N-acetylneuraminic acid is usually produced by fermentation. After fermentation, a series of post-treatments are carried out on the fermentation broth to obtain N-acetylneuraminic acid. A patented preparation of separation, purification and spray drying from microbial fermentation broth The method of N-acetylneuraminic acid dry powder solves the problem that N-acetylneuraminic acid cannot be widely used in cosmetics due to its high cost, and provides a method for preparing N-acetylneuraminic acid by separating, purifying and spray-drying from microbial fermentation broth. The method of amino acid dry powder reduces the production cost of N-acetylneuraminic acid, and obtains N-acetylneuraminic acid with stable quality, which meets the application requirements in the field of cosmetics

But the N-acetylneuraminic acid that this method obtains contains a certain amount of impurity, and the stability of the aqueous solution of the N-acetylneuraminic acid that obtains is relatively poor, after this N-acetylneuraminic acid is dissolved in water, carry out high-temperature sterilization again A large amount of N-acetylneuraminic acid will be lost during processing, or when the N-acetylneuraminic acid is dissolved in water, it will be sterilized with a 0.22um membrane at room temperature, and then placed at room temperature (25°C) for a long time Significant loss of N-acetylneuraminic acid also occurs

In the prior art, there is a lack of a simple and effective means to improve the stability of this type of N-acetylneuraminic acid

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