A method for rapid detection of bacterial endotoxin

A bacterial endotoxin, rapid technology, applied in the direction of measuring devices, material stimulation analysis, instruments, etc., to achieve fast analysis speed, good reproducibility, and overcome the effect of coffee ring effect

Active Publication Date: 2021-03-30
FUZHOU UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Nevertheless, the effective extraction or enrichment of endotoxins in complex samples will still be the biggest bottleneck faced by chromatography-mass spectrometry

Method used

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  • A method for rapid detection of bacterial endotoxin
  • A method for rapid detection of bacterial endotoxin
  • A method for rapid detection of bacterial endotoxin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] The preparation of zinc ferrite chitosan composite matrix comprises the following steps:

[0032] Step 1: Preparation of zinc ferrite: Weigh zinc oxide, sodium acetate, FeCl 3 ·6H 2 O and Na 3 Cit·2H 2 O was dissolved in ethylene glycol, and the resulting solution was transferred to a reaction kettle. The reaction kettle was placed in an oven at 200°C and reacted for 12 hours. The resulting magnetic product was washed with water and ethanol four times and then dried at room temperature, wherein zinc oxide, sodium acetate, FeCl 3 ·6H 2 O and Na 3 Cit·2H 2 The mass ratio of O is 1:7:3:0.01;

[0033] Step 2: Preparation of zinc ferrite-chitosan composite material: adding zinc ferrite and chitosan to acetic acid solvent, and stirring at constant temperature at 60° C. for 2 hours. The product was isolated using a magnet, washed with water and ethanol until near neutral. Vacuum drying the washed product at 50° C. to obtain a zinc ferrite chitosan composite material....

Embodiment 2

[0036] The preparation of zinc ferrite chitosan composite matrix comprises the following steps:

[0037] Step 1: Preparation of zinc ferrite: Weigh zinc oxide, sodium acetate, FeCl 3 ·6H 2 O and Na 3 Cit·2H 2 O was dissolved in ethylene glycol, and the resulting solution was transferred to a reaction kettle. The reaction kettle was placed in an oven at 200°C and reacted for 12 hours. The resulting magnetic product was washed with water and ethanol four times and then dried at room temperature, wherein zinc oxide, sodium acetate, FeCl 3 ·6H 2 O and Na 3 Cit·2H 2 The mass ratio of O is 1:8:4:0.03;

[0038] Step 2: Preparation of zinc ferrite-chitosan composite material: adding zinc ferrite and chitosan to acetic acid solvent, and stirring at constant temperature at 60° C. for 2 hours. The product was isolated using a magnet, washed with water and ethanol until near neutral. Vacuum drying the washed product at 50° C. to obtain a zinc ferrite chitosan composite material....

Embodiment 3

[0046] The preparation of zinc ferrite chitosan composite matrix comprises the following steps:

[0047] Step 1: Preparation of zinc ferrite: Weigh zinc oxide, sodium acetate, FeCl 3 ·6H 2 O and Na 3 Cit·2H 2 O was dissolved in ethylene glycol, and the resulting solution was transferred to a reaction kettle. The reaction kettle was placed in an oven at 200°C and reacted for 12 hours. The resulting magnetic product was washed with water and ethanol four times and then dried at room temperature, wherein zinc oxide, sodium acetate, FeCl 3 ·6H 2 O and Na 3 Cit·2H 2 The mass ratio of O is 1:9:5:0.02;

[0048] Step 2: Preparation of zinc ferrite-chitosan composite material: adding zinc ferrite and chitosan to acetic acid solvent, and stirring at constant temperature at 60° C. for 2 hours. The product was isolated using a magnet, washed with water and ethanol until near neutral. Vacuum drying the washed product at 50° C. to obtain a zinc ferrite chitosan composite material....

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Abstract

The invention provides a method for rapidly detecting bacterial endotoxin based on surface-assisted laser desorption ionization mass spectrometry. The method prepares a zinc ferrite chitosan compositeand takes the zinc ferrite chitosan composite as a matrix, and a method for rapidly detecting the bacterial endotoxin based on an organic mass spectrometry technology is developed to overcome a coffee-ring effect of a traditional organic matrix and facilitate the rapid detection of the bacterial endotoxin. The method has the advantages that no complex sample pretreatment is needed, the sample consumption amount is small (about 0.1muL), the sample preparation is simple, the time consumption is less, the salt tolerance is good, and the reproducibility is good and the like, and has a good application prospect in the fields of food and drug safety.

Description

technical field [0001] The invention relates to a method for rapidly detecting bacterial endotoxin based on surface-assisted laser desorption ionization mass spectrometry, in particular to its application in drug and food detection. Background technique [0002] Matrix-assisted laser desorption ionization mass spectrometry (MALDI-MS) is a new type of high-efficiency soft-ionization organic mass spectrometer, which is very simple and efficient both in theory and in design, and has become indispensable for the analysis of biological macromolecules in recent years. one of the tools. The instrument mainly consists of two parts: matrix-assisted laser desorption ionization source (MALDI) and time-of-flight mass analyzer (TOF). The principle of MALDI is to irradiate the co-crystallized film formed by the sample and the matrix with a laser, and the matrix absorbs the energy in the laser and transmits it to the sample molecules, so that the sample to be analyzed is vaporized and ion...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/63G01N23/2251
Inventor 林子俺欧阳丹吴杰谢更奇郑梓扬施涵强陈玉谊陈锋
Owner FUZHOU UNIV
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