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Beauveria brongniartii fungicide and application thereof

A technology of Beauveria bassiana and bacterial agents, applied in the field of agriculture, can solve problems such as inability to achieve control effects, and achieve the effects of ensuring production and protecting the ecological environment

Active Publication Date: 2019-05-17
INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] However, it has been found in field applications that artificially applied Beauveria bassiana has a certain natural attenuation in the field, and its infection of target insects requires a certain probability and time for the pathogenic process. Therefore, the ideal control effect is often not achieved

Method used

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  • Beauveria brongniartii fungicide and application thereof
  • Beauveria brongniartii fungicide and application thereof
  • Beauveria brongniartii fungicide and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] (1) Inoculate the spores of the bacterial seed into a liquid medium containing 3% sucrose, 1% peptone, 0.25% yeast extract powder, 0.01% magnesium sulfate, and the balance of water. Cultivate for 48-60 hours to obtain liquid seeds; The liquid culture is inoculated into a solid medium, which contains 35-60% of grain hulls, 35-60% of wheat bran, and 5-10% of corn flour. After 5-9 days of cultivation, the substrate is directly crushed or separated and extracted after drying. Spore powder

[0027] (2) Prepare the mixed adjuvant of sodium alkyl succinate sulfonate: hemicellulose: sodium alginate: chitosan = 15:5:4:1 according to the weight ratio;

[0028] (3) Mix 5 parts by weight of spore powder, 4 parts by weight of auxiliary agent and 10 parts by weight of peanut shell powder;

[0029] (4) Add 1 part by weight of 10% imidacloprid wettable powder to 20 parts by weight of attapulgite, and mix well;

[0030] (5) Mix the mixture obtained in steps (3) and (4) again to obtain Beauveri...

Embodiment 2

[0032] (1) Inoculate the spores of the bacterial seed into a liquid medium containing 3% sucrose, 1% peptone, 0.25% yeast extract powder, 0.01% magnesium sulfate, and the balance being water. Cultivate for 48-60 hours to obtain liquid seeds; The liquid culture is inoculated into a solid medium, which contains 35-60% of grain hulls, 35-60% of wheat bran, and 5-10% of corn flour. After 5-9 days of cultivation, the substrate is directly crushed or separated and extracted after drying. Spore powder

[0033] (2) Prepare the mixed adjuvant of sodium alkyl succinate sulfonate: hemicellulose: sodium alginate: chitosan = 15: 5: 4: 1 by weight ratio;

[0034] (3) Mixing 20 parts by weight of spore powder, 6 parts by weight of auxiliary agent and 10 parts by weight of corn cob powder;

[0035] (4) Add 2 parts by weight of 25% thiamethoxam wettable powder to 20 parts by weight of diatomaceous earth, and mix well;

[0036] (5) Mix the mixture obtained in steps (3) and (4) again to obtain Beauveri...

Embodiment 3

[0038] (1) Inoculate the spores of the bacterial seed into a liquid medium containing 3% sucrose, 1% peptone, 0.25% yeast extract powder, 0.01% magnesium sulfate, and the balance being water. Cultivate for 48-60 hours to obtain liquid seeds; The liquid culture is inoculated into a solid medium, which contains 35-60% of grain hulls, 35-60% of wheat bran, and 5-10% of corn flour. After 5-9 days of cultivation, the substrate is directly crushed or separated and extracted after drying. Spore powder

[0039] (2) Prepare the mixed adjuvant of sodium alkyl succinate sulfonate: hemicellulose: sodium alginate: chitosan = 15:5:4:1 according to the weight ratio;

[0040] (3) Mix 50 parts by weight of spore powder, 10 parts by weight of auxiliary agent and 70 parts by weight of bagasse powder;

[0041] (4) Add 3 parts by weight of 10% imidacloprid wettable powder to 60 parts by weight of attapulgite, and mix;

[0042] (5) Finally, 6 parts by weight of carboxymethyl cellulose and the mixture of t...

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Abstract

The invention belongs to the technical field of agriculture, and specifically relates to a beauveria brongniartii fungicide and application thereof. The beauveria brongniartii fungicide is prepared from beauveria brongniartii BRNS50206, a mixed adjuvant, a mineral carrier and a grain carrier, wherein the preservation number of the beauveria brongniartii BRNS50206 is CGMCC No. 15993. According to the beauveria brongniartii fungicide disclosed by the invention, beauveria brongniartii BRNS50206 is used as an active component of a fungicide, is specifically used for infecting insects but is safe to vertebrates, has no toxic and side effects, can be naturally blended with environment and is nondestructive and non-pollution, and the beauveria brongniartii fungicide is an environment-friendly andsafe biological pesticide. The beauveria brongniartii fungicide can be applied to preventing and treating injurious insects such as grubs, root weevil and cutworm of crops, forest, lawns and grass.

Description

Technical field [0001] The invention belongs to the field of agricultural technology, and specifically relates to a beauveria bassiana bacterial agent and its application. Background technique [0002] Underground pests include more than 200 species of grubs, root weevil, root leaf beetle, cutworm, mole cricket, root maggots, etc. They live in the soil at least one stage in their life cycle and feed on plants such as crops, forests, flowers, and pastures. Seeds, seedlings, rhizomes and underground fruits cause the lack of seedlings to fall, and the plants wilt or even die due to insufficient nutrient absorption. The damage is hidden and difficult to predict. [0003] For a long time, our country has mainly relied on chemical pesticides to prevent and control pests. Due to the large number of chemical pesticides applied year after year, agricultural products, soil and waters have been polluted by pesticide residues, which has posed a serious threat and damage to human health. The e...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01N51/00A01N43/56A01P7/04
Inventor 农向群王广君王庆雷蔡霓张泽华
Owner INST OF PLANT PROTECTION CHINESE ACAD OF AGRI SCI
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