Extracting method for soybean oligosaccharides
The technology of soybean oligosaccharide and extraction method is applied in the field of extraction of soybean oligosaccharide, which can solve the problems of difficult dissolution of soybean oligosaccharide, inability to separate soybean oligosaccharide, low extraction rate, etc., and achieves easy leaching and operation. The effect of short time and simple extraction process
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Embodiment 1
[0045] Such as figure 1 As shown, the invention discloses a method for extracting soybean oligosaccharides, comprising the following specific steps:
[0046] 1. Pretreatment: After the soybeans are crushed, extruded and extruded to obtain extruded soybeans, the extruded extruded and water are mixed, stirred and mixed evenly at a temperature of 45°C in an ultrasonic environment, and then enzyme hydrolyzate to obtain enzymatic hydrolyzate, and centrifuge the enzymolyzate to obtain free oil, emulsion, hydrolyzate and residue;
[0047] 2. Ultrasonic-microwave synergistic treatment: Add the emulsion, hydrolyzate and residue to absolute ethanol, stir and mix, and perform intermittent ultrasonic-microwave synergistic treatment to obtain a mixed solution;
[0048] 3. Acid precipitation treatment: add acid to the water phase waste liquid to adjust the pH to 4.5, carry out acid precipitation treatment, and then centrifuge to obtain the precipitate and the water phase mixture. The preci...
Embodiment 2
[0055] Such as figure 1 As shown, the invention discloses a method for extracting soybean oligosaccharides, comprising the following specific steps:
[0056] 1. Pretreatment: crush the soybeans and perform extrusion and puffing treatment to obtain extruded soybeans. After mixing the extruded puffed substances with water, stir and mix them evenly at a temperature of 50°C in an ultrasonic environment, and then perform enzyme hydrolyzate to obtain enzymatic hydrolyzate, and centrifuge the enzymolyzate to obtain free oil, emulsion, hydrolyzate and residue;
[0057] 2. Ultrasonic-microwave synergistic treatment: Add the emulsion, hydrolyzate and residue to absolute ethanol, stir and mix, and perform intermittent ultrasonic-microwave synergistic treatment to obtain a mixed solution;
[0058] 3. Acid precipitation treatment: add acid to the water phase waste liquid to adjust the pH to 4.5, carry out acid precipitation treatment, and then centrifuge to obtain the precipitate and the ...
Embodiment 3
[0065] Such as figure 1 As shown, the invention discloses a method for extracting soybean oligosaccharides, comprising the following specific steps:
[0066] 1. Pretreatment: crush the soybeans and perform extrusion processing to obtain extruded soybeans. After mixing the extruded extruded materials with water, stir and mix them evenly at a temperature of 55°C in an ultrasonic environment, and then perform enzyme hydrolyzate to obtain enzymatic hydrolyzate, and centrifuge the enzymolyzate to obtain free oil, emulsion, hydrolyzate and residue;
[0067] 2. Ultrasonic-microwave synergistic treatment: Add the emulsion, hydrolyzate and residue to absolute ethanol, stir and mix, and perform intermittent ultrasonic-microwave synergistic treatment to obtain a mixed solution;
[0068] 3. Acid precipitation treatment: add acid to the water phase waste liquid to adjust the pH to 4.5, carry out acid precipitation treatment, and then centrifuge to obtain the precipitate and the water phas...
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