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Bacterial nucleic acid sequencing and identification system and method for intelligent DNA barcodes

A bacterial nucleic acid and identification method technology, applied in the field of DNA sequencing and identification, can solve problems that hinder food safety early warning, food poisoning traceability pollution level and exposure risk research, and achieve the effect of ensuring food safety, facilitating technical research, and preventing poisoning incidents

Inactive Publication Date: 2019-05-07
青岛市疾病预防控制中心
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Problems solved by technology

The lack of identification standard database and the limitations of analytical methods have largely hindered food safety early warning, food poisoning traceability, and research on contamination levels and exposure risks

Method used

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  • Bacterial nucleic acid sequencing and identification system and method for intelligent DNA barcodes

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Embodiment Construction

[0023] The following will clearly and completely describe the technical solutions in the embodiments of the present invention with reference to the accompanying drawings in the embodiments of the present invention. Obviously, the described embodiments are only some, not all, embodiments of the present invention. Based on the embodiments of the present invention, all other embodiments obtained by persons of ordinary skill in the art without making creative efforts belong to the protection scope of the present invention.

[0024] like figure 1 As shown, the present invention provides a technical solution: an intelligent DNA barcode bacterial nucleic acid sequencing identification system, including an extraction module, a data generation module and a data detection module, the extraction module includes bacterial nucleic acid extraction and purification and bacterial nucleic acid sequencing operations, data generation The module includes the sequence generation of bacterial nucle...

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Abstract

The invention discloses a bacterial nucleic acid sequencing and identification system and method for intelligent DNA barcodes, and relates to the technical field of DNA sequencing and identification.Through the system and method, technical research on the DNA barcode database and detection and identification traceability thereof of food poisoning sources can be conveniently realized, so that foodsafety can be guaranteed, the meal selection of people can be guided, the occurrence of poisoning events can be prevented, and as the nucleic acid sequencing identification method of common bacteriais established, convenient DNA barcode sequencing and identification of bacteria, cells and viruses in the biological field can be achieved; operation can be performed on a DNA genome in a special wayduring extraction and purification, so that the dissociation of a DNA sequence can be avoided. Through the adoption of a constructed standard nucleic acid sequence, a standard nucleic acid sequence database for bacterial nucleic acid sequencing and identification can be input according to a unified common description and normative codes, so that objective and accurate judgment bases can be provided for the nucleic acid sequencing identification method.

Description

technical field [0001] The invention relates to the technical field of DNA sequencing identification, in particular to an intelligent DNA barcode bacterial nucleic acid sequencing identification system and method. Background technique [0002] Molecular identification of DNA signature sequence (DNA barcode) is a molecular biological classification method for species identification using a recognized and relatively short DNA sequence in the genome. Based on nucleic acid sequencing technology, this method establishes a database and identification platform by screening universal DNA characteristic sequences, uses bioinformatics methods to analyze and compare DNA data, and then identifies species, which is an effective supplement and important to traditional biological identification methods. In recent years, it has been widely concerned by drug quality standard setting agencies at home and abroad, and has gradually become a research hotspot in species identification and classif...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/689C12Q1/686C12Q1/04
Inventor 于维森石学香杜惠琴牛娟娟
Owner 青岛市疾病预防控制中心
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