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Preparation method and application of Elsholtzia rugulosa Hemsl spice for tobaccos

A technology of tobacco spices and wild bazi, which is applied in the fields of application, essential oils/flavors, tobacco, etc., can solve the problems of insufficient variety of flavors and fragrances for tobacco, insufficient preparation methods, etc., and achieve the safety and environmental protection of spices and processing technology, and facilitate industrialization The effect of production, good application value

Active Publication Date: 2019-05-07
CHINA TOBACCO YUNNAN IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The object of the present invention is to provide a method for preparing Yebazi tobacco spices with floral and herbal fragrance and its application in cigarettes, aiming at the problems that the types of flavors and fragrances for tobacco are not rich enough and the preparation method is insufficient. Tobacco yellow soil-derived bacteria X7X fermentation process is used to process wild bazi, and then a kind of wild bazi tobacco flavor is obtained through solvent extraction process, which is applied to cigarette shreds, so that the smoke has a special floral and herbal fragrance, and the fragrance is full and prominent , can reduce the irritation of cigarette smoke, enhance the flavoring effect of cigarettes, improve the smoking quality of cigarettes, and enrich the sources and types of flavors and fragrances for tobacco

Method used

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  • Preparation method and application of Elsholtzia rugulosa Hemsl spice for tobaccos
  • Preparation method and application of Elsholtzia rugulosa Hemsl spice for tobaccos
  • Preparation method and application of Elsholtzia rugulosa Hemsl spice for tobaccos

Examples

Experimental program
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Embodiment 1

[0036]1. Isolation, cultivation and identification of tobacco yellow soil-derived bacteria X7X

[0037] 1.1 Isolation of tobacco yellow soil-derived bacteria X7X

[0038] Soil samples were taken from the tobacco rhizosphere of the tobacco production base in Kunming, Yunnan Province, sealed in plastic bags, and stored at 4°C for later use. Accurately weigh 10g of soil sample, add 100mL of sterile water, shake and incubate at 30°C, 200rpm for 30min; dilute the concentration to 10 with sterile water -3 、10 -4 W / v times, take 0.1mL of the two concentrations of bacteria solution and evenly spread it on the R2A agar plate, set up three parallel experiments for each concentration of the bacteria solution, and cultivate it in a constant temperature incubator at 30°C for 2 weeks. Pick single colonies of different shapes from above, continue to use plate streaking method to isolate and purify the strains, inoculate them on R2A slant medium after numbering, or prepare glycerol suspensi...

Embodiment 2

[0056] 1. Cultivation of Tobacco Yellow Soil Bacteria X7X

[0057] (1) Test tube slant culture

[0058] Slope preservation medium is used, the medium is R2A agar medium, and the formula of the medium is: 0.5g of glucose, 0.5g of yeast extract, 0.5g of peptone, 0.5g of acid hydrolyzed casein, 0.5g of soluble starch, and 0.3g of sodium pyruvate , dipotassium hydrogen phosphate 0.3g, magnesium sulfate 0.05g, agar 15g, distilled water to 1000mL, pH7.2. Sterilize the culture medium at 121°C for 25 minutes, place it on a slant, inoculate tobacco yellow soil-derived bacteria X7X, and cultivate it at 30°C for 2 weeks to obtain test tube strains;

[0059] (2) Seed cultivation

[0060] The seed medium is R2A liquid medium, and the formula is: 0.5g glucose; 0.5g yeast extract; 0.5g peptone; 0.5g acid hydrolyzed casein; 0.5g soluble starch; 0.3g sodium pyruvate; Dipotassium 0.3g; magnesium sulfate 0.05g; distilled water 1000mL, pH7.2. Sterilize the culture medium at 121°C for 25 minut...

Embodiment 3

[0078] 1. The cultivation method of tobacco yellow soil-derived bacterium X7X is the same as embodiment 2.

[0079] 2. Fermentation of wild bazi flour

[0080] Grind the dried Yebazi plant into a powder with a particle size of 30 meshes, weigh 1000g of Yebazi powder, balance the moisture to 10% in advance, spray 200mL of tobacco yellow soil-derived bacteria X7X inoculum, mix well, put the processed Yebazi powder in Enter 22 DEG C, 60% constant temperature and humidity chamber fermentation 48h, obtain fermented wild bazi powder.

[0081] 3. Preparation of Yebazi concentrated extract

[0082] Put the obtained fermented wild seed powder in a sterilizing pot at 121 ° C for 20 minutes, add 95% ethanol with a concentration 15 times the mass of the fermented wild seed powder, soak and extract for 48 hours; filter the extract with a 200-mesh filter, and obtain The clear liquid is concentrated at 45°C and 70kPa until the volume does not change, and the concentrated extract of Yebazi ...

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Abstract

The invention provides a preparation method and application of an Elsholtzia rugulosa Hemsl spice for tobaccos, and the preparation method comprises the following steps: (1) preparing a tobacco yellowsoil source microbial agent X7X; (2) fermenting Elsholtzia rugulosa Hemsl powder by using the tobacco yellow soil source microbial agent X7X to obtain fermented Elsholtzia rugulosa Hemsl powder; (3)soaking the fermented Elsholtzia rugulosa Hemsl powder by using ethanol, and filtering and concentrating to obtain an Elsholtzia rugulosa Hemsl concentrated extracting solution; and (4) finely extracting the Elsholtzia rugulosa Hemsl concentrated extracting solution by macroporous resin to obtain the Elsholtzia rugulosa Hemsl spice for tobaccos. The Elsholtzia rugulosa Hemsl spice for tobaccos isapplied to cigarette tobacco shreds, so that smoke has special flower fragrance and herbal fragrance, the smoke irritation of cigarettes can be reduced, the fragrance-forming effect of the cigarettescan be enhanced, and the smoking quality of the cigarettes can be improved.

Description

technical field [0001] The invention belongs to the technical field of flavors and fragrances for cigarettes, and in particular relates to a preparation method and application of a flavors for Yebazi tobacco. Background technique [0002] Cigarette aroma is the core content of cigarette quality, but with the development of low-coke cigarettes, the reduction of cigarette smoking taste and lack of aroma have become prominent problems in the development of tobacco. Cigarette flavoring and addition is already a routine and necessary technical means to supplement the aroma. By means of flavoring and adding ingredients, it can make up for the deficiency in the formula of the cigarette leaf group, eliminate miscellaneous gases, and make the cigarette aroma richer and more sufficient. Therefore, it has a unique style in absorbing and smelling fragrance, and enhances the characteristics of the product. Natural flavors play an extremely important role in cigarette flavoring, and thei...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B9/00A24B15/30
Inventor 高莉段焰青夏建军李文均焦俊杜刚李先毅
Owner CHINA TOBACCO YUNNAN IND
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