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Preparation method and application of Nardostachys chinensis spice for tobaccos

A technology of pine tobacco and spices, which is applied in the direction of essential oils/fragrances, applications, tobacco, etc. It can solve the problems of insufficient preparation methods and insufficient types of flavors and fragrances for tobacco, and achieve environmental protection and safety of the processing technology, satisfying individualization and diversification demand, low-cost effects

Inactive Publication Date: 2019-05-07
CHINA TOBACCO YUNNAN IND
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a method for preparing and application of pine tobacco spices with woody and light fragrance to solve the problems that the types of flavors and fragrances for tobacco are not rich enough and the preparation methods are insufficient. Using tobacco yellow soil-derived bacteria X7X fermentation process and carbonization process to process pine, and then obtain a safe pine tobacco flavor through solvent extraction process, which can be used in cigarette shreds to make cigarette smoke have Special woody and delicate aroma, with a slightly unique burnt aroma, which can significantly enrich the cigarette aroma, increase the moistness of the smoke, coordinate the smoke, and improve the smoking quality of the cigarette

Method used

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  • Preparation method and application of Nardostachys chinensis spice for tobaccos
  • Preparation method and application of Nardostachys chinensis spice for tobaccos
  • Preparation method and application of Nardostachys chinensis spice for tobaccos

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Experimental program
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Embodiment 1

[0033] 1. Isolation, cultivation and identification of tobacco yellow soil-derived bacteria X7X

[0034] 1.1 Isolation of tobacco yellow soil-derived bacteria X7X

[0035] Soil samples were taken from the tobacco rhizosphere of the tobacco production base in Kunming, Yunnan Province, sealed in plastic bags, and stored at 4°C for later use. Accurately weigh 10g of soil sample, add 100mL of sterile water, shake and incubate at 30°C, 200rpm for 30min; dilute the concentration to 10 with sterile water -3 , 10 -4W / v times, take 0.1mL of the two concentrations of bacteria solution and evenly spread it on the R2A agar plate, set up three parallel experiments for each concentration of the bacteria solution, and cultivate it in a constant temperature incubator at 30°C for 2 weeks. Pick single colonies of different shapes from above, continue to use plate streaking method to isolate and purify the strains, inoculate them on R2A slant medium after numbering, or prepare glycerol suspens...

Embodiment 2

[0053] 1. Cultivation of Tobacco Yellow Soil Bacteria X7X

[0054] (1) Test tube slant culture

[0055] Slope preservation medium is used, the medium is R2A agar medium, and the formula of the medium is: 0.5g of glucose, 0.5g of yeast extract, 0.5g of peptone, 0.5g of acid hydrolyzed casein, 0.5g of soluble starch, and 0.3g of sodium pyruvate , dipotassium hydrogen phosphate 0.3g, magnesium sulfate 0.05g, agar 15g, distilled water to 1000mL, pH7.2. Sterilize the culture medium at 121°C for 25 minutes, place it on a slant, inoculate tobacco yellow soil-derived bacteria X7X, and cultivate it at 30°C for 2 weeks to obtain test tube strains;

[0056] (2) Seed cultivation

[0057] The seed medium is R2A liquid medium, and the formula is: 0.5g glucose; 0.5g yeast extract; 0.5g peptone; 0.5g acid hydrolyzed casein; 0.5g soluble starch; 0.3g sodium pyruvate; Dipotassium 0.3g; magnesium sulfate 0.05g; distilled water 1000mL, pH7.2. Sterilize the culture medium at 121°C for 25 minut...

Embodiment 3

[0076] 1. The cultivation method of tobacco yellow soil-derived bacterium X7X is the same as embodiment 2.

[0077] 2. Fragrant pine fermentation

[0078] Grind 1 kg of dried pine plants into powder with a particle size of 30 meshes, balance the moisture to 12%, spray 20 mL of tobacco yellow soil-derived bacteria X7X bacterial agent in every 100 g of pine powder, mix well, and place the processed pine powder in the Ferment for 48 hours in a 60% constant temperature and humidity box at 22° C. to obtain fermented pine.

[0079] 3. Carbonization of fermented pine

[0080]Put the fermented pine into the carbonization furnace, gradually increase the temperature of the furnace to about 80°C, and keep it for 20 minutes. After the fermented pine is dry, quickly raise the temperature to 120°C, and keep it for 1 hour. After checking the degree of carbonization, turn off the heat source and take it out. Samples of fermented carbonized pine for later use.

[0081] 4. Preparation of spi...

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Abstract

The invention provides a preparation method and application of a Nardostachys chinensis spice for tobaccos, and the preparation method comprises the following steps: (1) preparing a tobacco yellow soil source microbial agent X7X; (2) fermenting Nardostachys chinensis powder by using the tobacco yellow soil source microbial agent X7X to obtain fermented Nardostachys chinensis; (3) performing carbonization treatment on the fermented Nardostachys chinensis to obtain fermented carbonized Nardostachys chinensis; (4) using ethanol for refluxing extraction of the fermented carbonized Nardostachys chinensis, filtering and concentrating to obtain the Nardostachys chinensis spice for tobaccos. The Nardostachys chinensis spice for tobaccos is applied to cigarette tobacco shreds, so that smoke has special woody and clean fragrance, also has unique slight scorching fragrance, the fragrance of cigarettes can be obviously enriched, moistening of the smoke can be increased, the smoke can be harmonized, and the smoking quality of the cigarettes can be improved.

Description

technical field [0001] The invention belongs to the technical field of tobacco essence and fragrance, and in particular relates to a preparation method and application of pine tobacco fragrance. Background technique [0002] Cigarette aroma is the core content of cigarette quality, but with the development of low-coke cigarettes, the reduction of cigarette smoking taste and lack of aroma have become prominent problems in the development of tobacco. Cigarette flavoring and addition is already a routine and necessary technical means to supplement the aroma. By means of flavoring and adding ingredients, it can make up for the deficiency in the formula of the cigarette leaf group, eliminate miscellaneous gases, and make the cigarette aroma richer and more sufficient. Therefore, it has a unique style in absorbing and smelling fragrance, and enhances the characteristics of the product. Natural flavors play an extremely important role in cigarette flavoring, and their application ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B9/00A24B15/30
Inventor 段焰青郭青董立民夏建军黄立斌雷声徐若飞
Owner CHINA TOBACCO YUNNAN IND
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