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Centrifugal disc type micro-fluidic chip and application method thereof

A centrifuge disk and chip technology, applied in chemical instruments and methods, laboratory containers, and analysis by chemical reaction of materials, etc. The method is simple and convenient, the effect of reducing the interfacial tension and accurate detection

Active Publication Date: 2019-05-07
DONGGUAN HEC MEDICAL INTELLIGENT DEVICE R&D CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] A centrifugal disc microfluidic chip based on immunochemiluminescent reaction provided by the present invention solves the problem of difficult transfer of magnetic beads in the prior art, especially the problem of difficult transfer of micro-volume magnetic beads, and realizes that there is no need to increase the amount of magnetic beads, Magnetic bead transfer without additional magnetic force

Method used

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  • Centrifugal disc type micro-fluidic chip and application method thereof
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  • Centrifugal disc type micro-fluidic chip and application method thereof

Examples

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Embodiment 1

[0036] This embodiment provides a centrifugal disc microfluidic chip based on immunochemiluminescent reaction, including a chip body and a magnetic bead transfer module, and a plurality of centrifugal units are arranged on the chip body, and the centrifugal units include different fluid chambers. The fluid chamber contains reaction reagents, magnetic beads and organic reagents, the magnetic beads can move between different fluid chambers, and the organic reagents are used to reduce the viscosity and interfacial tension of the reaction reagents to facilitate the reaction between the magnetic beads and After the reagents are separated, they move to other fluid chambers, and the magnetic bead transfer module is used to provide magnetic driving force to transfer the magnetic beads to different fluid chambers.

[0037] Specifically, each of the centrifugal units can complete one detection index, thus, according to the configuration and combination of the centrifugal units, the joint...

Embodiment 2

[0069] In order to show that the addition of organic reagents will be beneficial to the transfer of magnetic beads, this example uses a centrifugal disc microfluidic chip based on immunochemiluminescent reaction as shown in Example 1 to carry out the following experiments. In this example, the organic reagent is isopropyl alcohol.

[0070] Mix 70 μL of magnetic bead dispersion (0.2857 mg / mL) of magnetic beads bound to biotinylated antibody, 70 μL of commercial alkaline phosphatase-labeled antibody solution (6 μg / ml) diluted with enzyme label diluent, and carcinoembryonic antigen (CEA ) solution (1000ng / mL) 10 μL was mixed in the centrifuge tube (referred to as the reaction mixture), and immediately added to the sample inlet chamber 33 from the sample inlet 43 on the chip body 1 . Fix the chip body 1 in the rotating shaft of the centrifugal device through the central hole on the chip body 1, and set the corresponding working parameters of the centrifugal device. The centrifuga...

Embodiment 3

[0075] In order to verify whether isopropanol has an adverse effect on the magnetic bead-based sandwich immunochemiluminescence reaction system used, this example will perform the magnetic bead-based sandwich immunochemiluminescence reaction in the centrifugal disk microfluidics after the completion of , take out the reaction solution from the chemiluminescent reaction chamber 38, and then perform signal detection on a microplate reader. The chip body 1 used in this embodiment is as Figure 5 As shown, the difference between its structure and the first embodiment is that the roof layer 4 is slightly different, and the rest are the same.

[0076] The top plate layer 4 of the chip body 1 in this embodiment is as Image 6 shown. The difference between the top plate layer 4 and the first embodiment is that it also includes a sampling hole 47 . The sampling hole 47 is located on the upper right side of the chemiluminescence reaction chamber 38, which is sealed by a film containi...

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Abstract

The invention provides a centrifugal disc type micro-fluidic chip which comprises a chip body and a magnetic bead transferring module, a plurality of centrifugal units are arranged on the chip body, the centrifugal units comprise different fluid chambers, each fluid chamber comprises a reaction reagent, magnetic beads and an organic reagent, the magnetic beads can move among different fluid chambers, the organic reagent is used for reducing the viscosity and interfacial tension of the reaction reagent, so that the magnetic beads can be conveniently separated from the reaction reagent and thenmove to other fluid chambers, the magnetic bead transferring module is used for providing magnetic driving force to enable the magnetic beads to be transferred into different fluid chambers. Accordingto the invention, the viscosity and interface tension of the reaction reagent are reduced by adding the organic reagent, the problem that magnetic beads are difficult to transfer in the prior art issolved, and particularly, the problem of difficulty in transferring the micro magnetic beads is solved, and the e magnetic beads can be transferred without increasing magnetic force and the magnetic bead amount.

Description

technical field [0001] The invention relates to the field of microfluidic chips, in particular to a centrifugal disc microfluidic chip based on immunochemiluminescent reaction and a method for using the same. Background technique [0002] Magnetic bead-based immunoassays are increasingly used on spin-disk microfluidic chips. In the centrifugal disc microfluidic chip, antibody-coated magnetic beads are usually used to capture and enrich the target antigen to be detected in the sample solution. When the target antigen to be tested is fully bound to the surface of the magnetic beads, it is necessary to use magnetic bead separation or magnetic bead transfer technology to separate the magnetic beads from the sample solution for subsequent operations or analysis and testing steps. Magnetic bead separation refers to making the magnetic beads gather at a certain position in the channel / chamber of the microfluidic chip in the flowing fluid, while the fluid flows out of the position ...

Claims

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Application Information

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IPC IPC(8): B01L3/00G01N21/76G01N33/543
Inventor 陈跃东张瑜顾志鹏刘仁源李建霖
Owner DONGGUAN HEC MEDICAL INTELLIGENT DEVICE R&D CO LTD
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