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Light-field microscope with selective-plane illumination

A light field microscope, flat technology, applied in the field of microscopy

Pending Publication Date: 2019-05-03
MOLECULAR DEVICES AUSTRIA GMBH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, experimental work so far has shown that light field imaging produces unacceptable amounts of image artifacts and background noise

Method used

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  • Light-field microscope with selective-plane illumination
  • Light-field microscope with selective-plane illumination
  • Light-field microscope with selective-plane illumination

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Embodiment Construction

[0023] As used herein, the term "sample" generally refers to any object or material that needs to be imaged and that can be mounted to the sample stage described herein. Samples can be biological (e.g., biological cells, tissues, organoids, spheroids, intracellular components, spores, fungi, molds, bacteria, viruses, and biologically derived particles such as skin cells, detritus) or abiotic (e.g., compounds, particulate matter, etc.). A sample may be a physiological material, a food sample, an environmental sample, etc. Samples may be partially or completely liquid or solid. Samples may be pretreated prior to use, such as preparing plasma from blood, diluting viscous fluids, sectioning samples, staining samples, adding reagents, adding fluorophores or other labels, and the like. As understood by those skilled in the art, pretreatment methods may also include filtration, precipitation, dilution, distillation, concentration, deactivation of interfering components, chromatogra...

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Abstract

A light-field microscope includes a light-sheet focusing device configured for outputting illumination light as a light sheet, and a microlens array between an objective lens and a light detector. A sample is irradiated by the light sheet along a direction non-parallel to the sample plane. Light-field data may be acquired from the sample without needing to scan through the thickness of the sample.The microscope implements light-field acquisition in conjunction with selective plane illumination.

Description

[0001] related application [0002] This application claims priority to US Patent Application No. 15 / 266,310, filed September 15, 2016, which is hereby incorporated by reference in its entirety. technical field [0003] The present invention relates generally to microscopy for imaging samples, including three-dimensional microscopy and fluorescence-based microscopy. In particular, the invention relates to microscopy for selective planar illumination in combination with the acquisition of light field information. Background technique [0004] Microscopy represents a class of analytical techniques involving the imaging of samples such as biological material (eg cells, tissues etc.) and non-biological material (eg particulate matter). Known light (optical) based microscopy techniques include, for example, bright field microscopy, dark field microscopy, confocal microscopy, and selective plan illumination microscopy (SPIM). Light-based microscopy techniques can be enhanced by ...

Claims

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Application Information

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IPC IPC(8): G02B27/00G02B21/00G02B21/36
CPCG02B27/0075G02B21/0032G02B21/008G02B21/367G01N21/6458G01N2201/0612G01N2201/062G01N2201/0636G01N2201/0638G02B21/082G02B21/16G02B21/26G02B21/361G02B21/365
Inventor 约瑟夫·阿茨勒安德烈亚斯·肯达
Owner MOLECULAR DEVICES AUSTRIA GMBH
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